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Air-dried amniotic membrane powder and application

An amniotic membrane, air-drying technology, applied in powder delivery, medical raw materials derived from mammals, drug combinations, etc., can solve problems such as disease transmission, inconvenience in use, and difficulty in sterilization

Inactive Publication Date: 2021-08-20
胡竹林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Glycerol preservation of amniotic membrane can fully preserve the histological structure and cytokine content of amniotic membrane, but it is difficult to sterilize, which may lead to disease transmission
[0005] Although freeze-dried amnion can retain growth factor activity better, its activity is still lower than that of fresh amnion, and the mechanical strength of freeze-dried amnion is weak. significant loss
[0006] Amniotic membrane extract is another hot spot in the research and development of new amniotic membrane products. It has a variety of high-level growth factors, but the amniotic membrane extract is easy to flow out from the nasolacrimal duct when it is instilled in the eyes, and its bioavailability is low. It is inconvenient to use at night and has a short shelf life.
[0007] The new composite amniotic membrane combines the amniotic membrane with absorbable biomaterials with excellent properties, which can improve its mechanical strength and plasticity while retaining the excellent properties of the amniotic membrane itself, but the complex chemical process will increase the risk of inflammatory reactions, and most of them are still in the experimental stage. stage

Method used

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  • Air-dried amniotic membrane powder and application
  • Air-dried amniotic membrane powder and application
  • Air-dried amniotic membrane powder and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation method of air-dried amnion powder, comprises the following steps:

[0030] S1. Obtain amniotic membranes. Select cesarean section pregnant women who are negative for HIV, HBV, HCV, syphilis and other infectious diseases. Obtain discarded placenta within 24 hours after delivery, and bluntly separate the amniotic membranes.

[0031] S2, wash the blood clots and mucus on the amniotic membrane with sterile normal saline, and then place it in normal saline containing 50ug / ml penicillin-streptomycin, 100ug / ml neomycin, and 215ug / ml amphotericin B for 45min repeatedly. , and finally rinsed 3 times with sterile saline.

[0032] S3, air-dry the amniotic membrane, flatten the washed amniotic membrane, fix it on a support frame, put it into a 37°C constant temperature circulating air drying oven, and let it stand for 2 hours to fully remove the water to obtain the air-dried amniotic membrane.

[0033] S4, Grind into powder, put the amnion tissue drie...

Embodiment 2

[0042] Example 2: Amniotic membrane-fibrin cement, amniotic membrane-fibrin cement (AM-FS: Amniotic membrane-Fibrin Sealant) containing different concentrations of amniotic membrane powder, the preparation steps are as follows:

[0043] S1, fully mix the amniotic membrane powder prepared in Example 1 with the catalyst solution in pig fibrin glue, and prepare respectively. ml, 4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0mg / ml amnion powder-catalyst mixture.

[0044] S2, sequentially take 50ul of amnion powder-catalyst with the above concentration, respectively add them to 24-well cell culture plates, spread them evenly on the bottom of the culture plates, add an equal volume of main glue solution to each of the above culture wells in turn, oscillate gently, mix evenly, and form According to the mass ratio, the concentration of amniotic membrane powder is: 65mg / ml, 32mg / ml, 16mg / ml, 8mg / ml, 4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0.125mg / ml ml, 0mg / ml AM-FS cement.

[004...

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Abstract

The invention discloses air-dried amniotic membrane powder and application thereof, relates to an amniotic membrane product, and particularly relates to a preparation method and application of amniotic membrane powder. The air-dried amniotic membrane powder is prepared by air-drying a fresh sterile and virus-free amniotic membrane at a constant temperature of 36-37 DEG C and grinding the amniotic membrane into the amniotic membrane powder. The air-dried amniotic membrane powder disclosed by the invention is simple and convenient to prepare, stable in property at room temperature and easy to store, and is a convenient and effective amnion product. The AM-FS daub prepared from amniotic membrane powder is prepared by loading amniotic membrane powder by using fibrin glue as a transfer matrix, contains abundant growth factors from amniotic membrane tissues, combines excellent biological properties of the fibrin glue, has an ocular surface tissue damage repairing effect similar to that of a fresh amniotic membrane, and overcomes the defectsthat the fresh amniotic membrane needs to be sutured, the surgical operation difficulty is high, and the operation fails due to easy falling, and the air-dried amniotic membrane powder is suitable for repairing various types of eye surface tissues and skin tissue injuries.

Description

technical field [0001] The invention relates to an amniotic membrane product, in particular to a preparation method and application of amniotic membrane powder. Background technique [0002] The amniotic membrane is located in the innermost layer of the fetal membrane. It has anti-inflammatory, anti-angiogenesis, immune regulation, anti-bacterial, anti-fibrosis, good tissue compatibility and other properties. It is often used as a substitute for epithelial basement membrane and is widely used in the repair of ocular epithelial tissue , especially the treatment of chemical burns on the ocular surface. The application of amnion in the treatment of eye diseases is limited by factors such as the difficulty of obtaining fresh amnion, difficulty in long-term storage, thin texture, and complicated suturing operations. Finding a new type of amnion product that can retain the excellent biological properties of amnion and is easy to store and use has always been a research hotspot. ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K35/50A61K47/42A61P17/02A61P27/02
CPCA61K35/50A61K9/146A61P27/02A61P17/02
Inventor 张文佳胡竹林李楠钰和丹刘海孔蕾
Owner 胡竹林
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