Preparation method of targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy

A nano-drug carrier and photothermal synergistic technology, applied in nanotechnology, drug combination, pharmaceutical formulation, etc., can solve the problems of short cycle time and inability to fully exert its effect, achieve efficient accumulation, improve biocompatibility, and enrich pores structure effect

Pending Publication Date: 2021-08-24
SHIHEZI UNIVERSITY
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Problems solved by technology

[0005] Mesoporous silica has the characteristics of high porosity, high specific surface area, thermal stability, large drug loading capacity, stable structure, good biocompatibility, and no toxic and side effects, but its circulation time in the body is short and cannot Fully functioning, and a single mesoporous silica loaded into the human body, after the immune system cleared, only a very small amount of nanoparticles accumulated in the tumor tissue

Method used

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  • Preparation method of targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy
  • Preparation method of targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy
  • Preparation method of targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy

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preparation example Construction

[0042] A method for preparing a targeted long-circulation nano drug carrier for photothermal synergistic chemotherapy, comprising the following steps:

[0043] (1) preparing mesoporous silica;

[0044] (2) Mesoporous silica coated with polydopamine;

[0045] (3) Loading anticancer drugs: Add the coated mesoporous silica and anticancer drugs obtained in the step (2) into water, and stir for 12 hours to obtain the mesoporous silica loaded with anticancer drugs. silicon;

[0046] (4) Modification of erythrocyte membrane: distearoylphosphatidylethanolamine-polyethylene glycol-biotin and broken erythrocyte membrane were stirred at 4°C for 12 hours, and then extruded continuously through a 200nm filter membrane with a micro extruder. Obtain biotin-modified erythrocyte membrane;

[0047] (5) Add the biotin-modified erythrocyte membrane and the mesoporous silica loaded with anticancer drugs into the PBS solution, and after fully dispersing, filter with a micro extruder to obtain bi...

Embodiment 1

[0063] Concrete operation steps are as follows: (taken material amount " part " is weight part)

[0064] (1) Preparation of mesoporous silica:

[0065] Add 2 parts of n-amyl alcohol and 4 parts of TEOS (tetraethyl orthosilicate) into 30 parts of cyclohexane and stir evenly, add 2.44 parts of CTAB (cetyltrimethylammonium bromide) and 30 parts of deionized water , transferred to a polytetrafluoroethylene reactor after stirring. Place it in an oven at 120°C for 2 hours, wash it with deionized water three times, and dry it in the air at 70°C for one day to obtain a white powder, and then sinter it in a muffle furnace at 550°C for 6 hours to obtain a white powder of mesoporous bismuth Silicon oxide (MSN).

[0066] (2) Mesoporous silica coated with polydopamine:

[0067] After fully mixing and dissolving 5 parts of the prepared alkaline Tris HCl solution and 2 parts of dopamine powder, add 1 part of the prepared mesoporous silica nanoparticles, react under high-speed stirring for...

Embodiment 2

[0079] Using the method of step (1) in Example 1, on the basis of adjusting the time required for dopamine polymerization and the ratio of reactants, mesoporous silica nanoparticles coated with polydopamine were prepared.

[0080] The drug-loading performance of the prepared mesoporous silica nanoparticles coated with polydopamine was tested, and the results are as follows: figure 1 As shown, the highest drug loading can reach about 11%. Due to the coating, the specific surface area and pore volume of mesoporous silicon with large pore size are reduced. Compared with uncoated mesoporous silicon, the drug loading capacity is reduced, but it still has a higher drug loading capacity. Regulate different concentrations of anticancer drug DOX, and finally get the maximum drug loading. The concentration of DOX is 350 μg / mL, and the drug loading effect is good, which provides the possibility for future loading of drugs or other macromolecules and proteins.

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Abstract

The invention relates to a preparation method of a targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy. The invention relates to a preparation method of a targeted long-circulation nano-drug carrier for photo-thermal synergistic chemotherapy. The preparation method comprises the following steps: (1) preparing mesoporous silica; (2) coating the mesoporous silica with polydopamine; (3) loading an anti-cancer drug; (4) modifying the erythrocyte membrane by adopting biotin; (5) wrapping the mesoporous silica loaded with the anti-cancer drug by using the biotin-modified erythrocyte membrane. According to the invention, local photo-thermal / chemotherapy synergistic treatment of cancer tissues is realized through the PDA coating, so the treatment efficiency is improved; through wrapping of the erythrocyte membrane, the synthesized nanoparticles can effectively avoid a biological barrier, blood circulation of the whole body is carried out, and efficient accumulation of the nanoparticles in tumors is realized; the active targeting is carried out by modifying biotin, so the aggregation of the drug carrier at a tumor part is further improved, the biocompatibility of the drug carrier is greatly improved, and the circulation time of the drug carrier in a human body is greatly prolonged.

Description

technical field [0001] The invention belongs to the field of nano-medicine carrier materials, in particular to a method for preparing targeted long-circulation nano-drug carriers for photothermal synergistic chemotherapy. Background technique [0002] The "2018 Global Cancer Report" released by the World Health Organization shows that the number of new cases in my country is 3.804 million and the number of deaths is 2.296 million, ranking first in the world. Cancer prevention and treatment has become a national strategy and has a major demand . Finding more effective cancer treatment methods has become a major issue that needs to be tackled. At present, chemotherapy and radiotherapy are the two mainstream treatment methods at this stage, but their further development is limited by their low efficiency and severe side effects. [0003] The research on nano-drug carriers is expected to solve the problems of low efficiency and serious side effects. It has designable physical, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69
CPCA61K41/0052A61K31/704A61K47/6949A61K47/6897A61K47/545A61K47/60A61K47/34A61P35/00C01B33/18B82Y40/00A61K2300/00
Inventor 杨盛超崔林张一凡刘志勇吴建宁孟桂花李文娟林富丽乔智强
Owner SHIHEZI UNIVERSITY
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