Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for extracting proanthocyanidins from Aronia adenocarpa

A technology of proanthocyanidins and rowan fruit, applied in the field of plant active ingredient extraction, can solve the problems of unable to release proanthocyanidins and low extraction rate, achieve large-scale and industrial production, increase extraction rate, and extend the industrial chain.

Active Publication Date: 2022-06-03
秦皇岛惠恩生物技术有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method can only extract 89.71mg proanthocyanidins from 5g Aronia fruit, the extraction rate is 1.8%, the extraction rate is low, and it cannot release the proanthocyanidins in Aronia fruit to the greatest extent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting proanthocyanidins from Aronia adenocarpa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] see figure 1 , the present embodiment extracts the method for proanthocyanidins from Aronia nigra fruit, the steps are as follows:

[0050] 1) Raw material crushing and pulping

[0051] The fruit of Rowan nigra is mechanically mashed into pulp and passed through a 100-mesh sieve to obtain juice and pomace.

[0052] 2) Microbial fermentation of pomace

[0053] Add deionized water to the fermenter, prepare liquid culture medium according to 0.2g / L peptone, 2g / L sodium chloride, 10g / L magnesium sulfate and 5g / L dipotassium hydrogen phosphate, and then make the above-mentioned pomace according to 200g / L The dosage is put into the liquid medium.

[0054] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid medium with pomace, the inoculum amounts are 0.5%, 0.2% and 0.2% of the pomace amount, respectively; the fermentation temperature is 30°C, and the aeration rate is 1:1.0 , under the conditions of a pressure of 0.05 MPa and a rotati...

Embodiment 2

[0061] The present embodiment extracts the method for proanthocyanidins from Rowan nigra fruit, the steps are as follows:

[0062] 1) Raw material crushing and pulping

[0063] The fruit of Aronia nigra is mechanically mashed into pulp and passed through a 200-mesh sieve to obtain juice and pomace.

[0064] 2) Microbial fermentation of pomace

[0065] Add deionized water to the fermenter, prepare liquid medium according to 10g / L peptone, 0.2g / L sodium chloride, 0.2g / L magnesium sulfate and 10g / L dipotassium hydrogen phosphate, and then add the pomace of step 1). According to the dosage of 500g / L, it was put into the above-mentioned liquid culture medium.

[0066] Inoculate Aspergillus niger, Trichoderma viride and Bacillus licheniformis into the liquid medium with pomace, the inoculum amounts are 0.1%, 0.1% and 1% of the pomace amount, respectively; the fermentation temperature is 28°C, and the ventilation rate is 1:1.2 , under the conditions of a pressure of 0.04 MPa and a...

Embodiment 3

[0074] The present embodiment extracts the method for proanthocyanidins from Rowan nigra fruit, the steps are as follows:

[0075] 1) Raw material crushing and pulping

[0076] After mechanically mashing the fruit of Aronia nigra into pulp and passing through a 120-mesh sieve, juice and pomace are obtained.

[0077] 2) Microbial fermentation of pomace

[0078] Add deionized water to the fermenter, prepare liquid medium according to 2g / L peptone, 10g / L sodium chloride, 5g / L magnesium sulfate and 0.2g / L dipotassium hydrogen phosphate, and then make the pomace in step 1). The dosage of 300g / L was put into the above-mentioned liquid culture medium.

[0079] Aspergillus niger, Trichoderma viride and Bacillus licheniformis were inoculated into the liquid medium with pomace added, and the inoculation amounts were 0.3%, 0.5% and 0.5% of the pomace amount, respectively; the fermentation temperature was 32°C, and the ventilation rate was 1:1.1 , under the conditions of a pressure of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for extracting proanthocyanidins from Aronia adenocarpa berries. The method comprises: A) mashing and filtering the fruit of Aronia nigra, to obtain fruit juice and pomace; B) fermenting the pomace by using Aspergillus niger, Trichoderma viride and Bacillus licheniformis to obtain a pomace fermentation liquid; C) mixing fruit juice and pomace fermentation liquid to obtain a mixed liquid, using pectinase and papain to enzymolyze the mixed liquid to obtain an enzymatic hydrolyzate; D) separating and purifying the enzymatically hydrolyzed liquid to obtain a proanthocyanidin extract. The above method can degrade the pomace cell wall to facilitate the dissolution of proanthocyanidins, thereby extracting the proanthocyanidins from the pomace to the greatest extent, the whole process conditions are mild, the required equipment is simple, and large-scale and industrial production can be realized; at the same time, the method can The waste utilization of Sorbus arvensis pomace has promoted the development of the deep processing industry of Sorbus arvensis and enhanced the added value of Sorbus arvensis.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient extraction, in particular to a method for extracting proanthocyanidins from Rowan nigra fruit. Background technique [0002] Proanthocyanidins (OPC) are a kind of polyphenolic compounds widely found in nature. Studies have shown that proanthocyanidins have powerful antioxidant effects and can scavenge free radicals. In recent years, they have been found to have various effects such as prevention and treatment of cardiovascular diseases, anti-radiation, and immune regulation. At present, procyanidins are widely used in the fields of food, medicine and cosmetics as nutritional fortifiers, natural antioxidants, natural preservatives, etc. [0003] Aronia nigra, also known as aronia berry and raspberry, is native to North America and is now cultivated in Northeast my country, Inner Mongolia, Gansu and other provinces and cities. It is a Rosaceae with edible, medicinal, garden and ecol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C07G99/00
CPCC07D311/62C07G99/00Y02P20/54
Inventor 高小佳额尔敦巴雅尔朱孔山张洪敏符琳琳刘洪钧葛振宇徐志文
Owner 秦皇岛惠恩生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com