Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vibrio fluvialis bacteriophage YZU.V.F.P-21612C and application thereof

A technology of YZU.V.F.P-21612C and Vibrio riverina, which is applied in the field of bacteriostasis and enterobacter phage, can solve the problems of insufficient research and insufficient research on bacteriostatic agents, so as to reduce pollution, reduce transmission and cause Effect of Foodborne Illness Risk

Active Publication Date: 2021-09-03
YANGZHOU UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not enough researches on Vibrio riverina phage at home and abroad, and the research on efficient and specific antibacterial agent of Vibrio riverina phage is still insufficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vibrio fluvialis bacteriophage YZU.V.F.P-21612C and application thereof
  • Vibrio fluvialis bacteriophage YZU.V.F.P-21612C and application thereof
  • Vibrio fluvialis bacteriophage YZU.V.F.P-21612C and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Phage isolation and purification preparation

[0037] Phage isolation

[0038] Sewage samples were collected from the farmer's market in Yangzhou City, Jiangsu Province. Take 30mL sewage sample into a 50mL centrifuge tube, centrifuge at 5000×g for 10min, take 5mL supernatant and add it to 5mL LBS liquid medium, and add 100μL of Vibrio fluviobacter strain CICC21612 in the logarithmic growth phase at the same time, 37°C, 100rpm shaker Cultivate overnight; transfer the culture in the test tube to a sterile centrifuge tube, centrifuge at 5000×g for 10 min at 4°C, and pass the supernatant through a 0.22 μm filter membrane to obtain the phage stock solution.

[0039] Use SM buffer (1L: NaCl 5.8g, MgSO4.7H2O 2.0g, 1M Tris-HCl (pH7.4) 50mL) to carry out 10 gradient dilutions of the phage stock solution according to the volume ratio, and take 100 μL of the diluted phage suspension and 100 μL logarithmic Mix the vibrio in the growth phase for 10 minutes at room temperature, the...

Embodiment 2

[0049] Bacteriostatic effect of phage YZU.V.F.P-21612C in culture medium

[0050] Vibrio riverine (strain CICC21612) was pressed by 10 5 CFU / mL is inoculated into 30mL LBS liquid culture medium, adds phage YZU.V.F.P-21612C and VmYZU10474 (pathogenic Vibrio phage VmYZU10474 in the patent CN 112063593A) suspension to 10 in the phage treatment group respectively. 9 PFU / mL, an equal volume of SM buffer (0PFU / mL) was added to the negative control group, and the treated inoculum was placed in a 37°C incubator for constant temperature cultivation, and 1 mL was sampled every 2 hours, and the total number of colonies was measured by the coating method , 3 parallels were set for each group, and the average value was used for analysis.

[0051] The implementation results are as follows Figure 4 As shown, as the culture time prolongs, the total number of bacterial colonies in the negative control group increases rapidly, reaching 10 in 12 hours. 8 CFU / mL, while the total number of col...

Embodiment 3

[0053] Inhibitory Effect of Bacteriophage YZU.V.F.P-21612C on Vibrio riverine Biofilm

[0054] Take the Vibrio riverina culture (Vibrio riverina CICC21612 bacterium liquid) in the logarithmic growth phase, dilute it with LBS medium, and adjust the final concentration to be 1×10 7 CFU / mL. The test was divided into YZU.V.F.P-21612C treatment group, negative control group and blank group. YZU.V.F.P-21612C treatment group: Add 150 μL to each well of a 96-well plate with a concentration of 10 7 CFU / mL Vibrio riverina (CICC21612) bacterial liquid and 150 μL phage YZU.V.F.P-21612C suspension (A treatment was phage 105 PFU / mL, B treatment is 10 6 PFU / mL, C treatment is 10 7 PFU / mL, D treatment is 10 8 PFU / mL, E treatment is 10 9 PFU / mL); Negative control group: Add bacterial solution (10 7 CFU / mL) and 150 μL each of SM buffer; blank group: add 300 μL LBS medium to each well; incubate at 37°C for 24 hours, take out the 96-well plate, suck out the suspended bacteria, wash 3 times ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a vibrio fluvialis bacteriophage YZU.V.F.P-21612C and application thereof, the bacteriophage is identified to have unique genome structural characteristics, belongs to a novel bacteriophage and is preserved in the China Center for Type Culture Collection, the preservation time is December 7, 2020, and the preservation number is CCTCC NO: M2020867. The vibrio fluvialis bacteriophage YZU.V.F.P-21612C obtained by the invention has efficient lysis activity on vibrio fluvialis, can reduce or inhibit the formation of a biofilm of the vibrio fluvialis, can be used as a bacteriostatic agent to control the reproduction and metabolism of the vibrio fluvialis in the environments such as aquatic product temporary culture water or processing environment, appliances and the like, reduces and removes the biofilm formed in the environment or on the appliances, the pollution and transmission of vibrio fluvialis are effectively reduced, so that the culture and food safety risks are reduced.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to an enterobacterium hallii bacteriophage YZU.P.A-5 and its antibacterial application as a biological antibacterial agent in food production and storage environments. Background technique [0002] V.fluvialis is a kind of halophilic Vibrio, which widely exists in the water of rivers or estuaries, and can cause diseases in various aquatic animals such as fish, shrimp and shellfish. Huge economic loss. Vibrio riverine is also a food-borne pathogen. The human body is infected by ingesting contaminated water and food. It mainly causes acute gastrointestinal inflammation in humans. Typical symptoms are watery stools, abdominal pain, mild to moderate dehydration, and often accompanied by With fever, large-scale outbreaks have been caused in Bangladesh and India, and occasionally reported in the United States. At present, the treatment and prevention of bacterial diseases in aq...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A01N63/40A01P1/00C12R1/92
CPCC12N7/00A01N63/40C12N2795/10021C12N2795/10031
Inventor 陈静王莲莲吴钇萱郝贵杰高璐
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com