Conductive and degradable multifunctional tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold and dopamine technology, applied in the field of biomedical materials, can solve problems such as non-degradability of polypyrrole, and achieve the effects of good biological safety, improved safety and good dispersibility

Active Publication Date: 2022-07-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another example, Chinese patent CN201610166842 discloses a method for preparing a nano-polypyrrole chitin nerve guide, although it also discloses a preparation method for a conductive nerve guide, but there is still the problem that polypyrrole is not degradable

Method used

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  • Conductive and degradable multifunctional tissue engineering scaffold and preparation method thereof
  • Conductive and degradable multifunctional tissue engineering scaffold and preparation method thereof
  • Conductive and degradable multifunctional tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of conductive and degradable multifunctional tissue engineering scaffolds

[0056] 1. Preparation of two-dimensional phosphorus-based nanosheets (BP)

[0057] Weigh 10 mg of bulk crystal BP (when the bulk raw material is too large, conventional treatments such as grinding can be used to control the quality), clamp it on the electrode clip as the cathode, the platinum sheet is the anode, and dissolve 0.01 mol / L of propylene carbonate in 15 mL. tetra-n-butylammonium hydrogen sulfate (TBA HSO 4 ) as an electrolyte solution, the phosphor-based bulk was stripped off by applying a voltage of -5V through an electrochemical workstation for 30 min, followed by ultrasonic treatment in a water bath for 1 h, and then centrifuged at 1500 rpm for 20 min and the supernatant was taken to obtain a suspension of two-dimensional BP nanosheets; The suspension of BP nanosheets was centrifuged at 5000rpm for 20min and the precipitate was taken to obtain two-dimensional...

Embodiment 2

[0063] Example 2 Preparation of conductive and degradable multifunctional tissue engineering scaffolds

[0064] 1. Preparation of two-dimensional phosphorus-based nanosheets (BP)

[0065] Weigh 10 mg of bulk crystal BP, clamp it on the electrode clip as the cathode, the platinum sheet as the anode, and dissolve 0.01 mol / L TBA·HSO in 15 mL of propylene carbonate. 4 As the electrolyte solution, the phosphorous-based bulk was peeled off by applying a voltage of -5V through an electrochemical workstation for 30 min, followed by sonication in an ice-water bath for 1.5 h, then centrifuged at 2000 rpm for 15 min and the supernatant was taken to obtain a suspension of two-dimensional BP nanosheets; The suspension of dimensional BP nanosheets was centrifuged at 7000rpm for 15min and the precipitate was taken to obtain 2D BP nanosheets;

[0066] The two-dimensional BP nanosheets were ultrasonically dispersed in a small amount of anhydrous ethanol, and the added amount of anhydrous etha...

Embodiment 3

[0071] Example 3 Preparation of conductive and degradable multifunctional tissue engineering scaffolds

[0072] 1. Preparation of two-dimensional phosphorus-based nanosheets (BP)

[0073] Weigh 10 mg of bulk crystal BP, clamp it on the electrode clip as the cathode, the platinum sheet as the anode, and dissolve 0.01 mol / L TBA·HSO in 15 mL of propylene carbonate. 4 As the electrolyte solution, the phosphor-based bulk was exfoliated by applying a voltage of -5V through an electrochemical workstation for 30 min, followed by sonication in an ice-water bath for 2 h, and then centrifuged at 3000 rpm for 10 min and the supernatant was taken to obtain a suspension of two-dimensional BP nanosheets; The suspension of BP nanosheets was centrifuged at 8000rpm for 10min and the precipitate was taken to obtain two-dimensional BP nanosheets;

[0074] The two-dimensional BP nanosheets were ultrasonically dispersed in a small amount of anhydrous ethanol, and the added amount of anhydrous etha...

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Abstract

The invention belongs to the technical field of biomedical materials, and discloses a conductive and degradable multifunctional tissue engineering scaffold and a preparation method thereof, wherein a precursor for forming a conductive and degradable tissue engineering scaffold comprises two-dimensional phosphorus-based nanomaterials, Biopolymer materials and cross-linking agents, wherein bio-polymer materials and cross-linking agents are used to form the framework of hydrogel scaffolds, and two-dimensional phosphorus-based nanomaterials are used to be loaded on the interior or surface of hydrogel scaffolds to improve the scaffolding conductivity. The present invention improves the key components of the scaffold and its precursor, introduces two-dimensional phosphorus-based nanomaterials, cooperates with biopolymer materials (such as natural polymer materials naturally occurring in nature), and participates in the formation of the conductive polymer in the present invention. By degrading the multifunctional tissue engineering scaffold and its precursor, the obtained tissue engineering scaffold has the functions of electrical conductivity and degradability, and can guide nerve regeneration, and greatly promote the functional recovery of nerve injury areas.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and more particularly relates to a conductive and degradable multifunctional tissue engineering scaffold and a preparation method thereof. Background technique [0002] With the development of the economic level of all countries in the world, the incidence of spinal cord injury is increasing year by year. Spinal cord injury is the most serious complication of spinal cord injury, often resulting in severe functional impairment of the limbs below the injured level. Spinal cord injury will not only bring serious physical and psychological harm to the patient himself, but also cause a huge economic burden to the whole society. Due to the socioeconomic loss caused by spinal cord injury, prevention, treatment and rehabilitation of spinal cord injury has become a major topic in the medical field today. [0003] Implantable scaffolds are an effective strategy to provide cellular support. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/12A61L27/50A61L27/52A61L27/58C08B37/08C01B25/02C01B25/08C01B33/00
CPCA61L27/20A61L27/222A61L27/12A61L27/52A61L27/50A61L27/58C08B37/0072C01B25/02C01B25/08C01B33/00A61L2430/32A61L2400/12C08L5/08C08L5/04C08L89/00
Inventor 罗志强徐超
Owner HUAZHONG UNIV OF SCI & TECH
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