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Novel phomopsis fungus strain, preparation method and application

A technology of Phomopsis genus and new strains, applied in the field of microorganisms, can solve problems such as no research reports on endophytic fungi in Yunnan shizi, achieve broad-spectrum antibacterial activity and antioxidant activity, realize effective utilization, and strong scavenging ability Effect

Active Publication Date: 2021-10-08
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yunnan Shizi has high medicinal and economic value, but the current research on this plant mainly focuses on the pharmacological activity of its extract and the isolation and identification of chemical components, and there has been no research report on the endophytic fungi of Yunnan Shizi

Method used

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  • Novel phomopsis fungus strain, preparation method and application
  • Novel phomopsis fungus strain, preparation method and application
  • Novel phomopsis fungus strain, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and cultivation of new strains of Phomopsis fungus, including:

[0045] (1) Rinse the leaves of the freshly collected, asymptomatic Yunnan stone catalpa with tap water, and remove the soil and dust attached to the surface. Put the dried plant material into an ultra-clean workbench sterilized by ultraviolet rays, and select 75% alcohol and 2% sodium hypochlorite as surface disinfectants. First soak the plant material with 75% alcohol for 30-50s, rinse with sterile water for 2-3 times; then treat it with 2% sodium hypochlorite for 1-2min, and rinse with sterile water for 3 times. Blot dry with sterile filter paper.

[0046] (2) Cut the sterilized leaves of Catalpa yunnanensis into small pieces of about 1×1 cm, inoculate the plant leaf tissue on PDA medium, and culture at a constant temperature of 28±2°C. The PDA medium includes 200 g of peeled potatoes, 20 g of glucose, 15 g of agar, 1000 mL of distilled water, and a natural pH.

[0047] (3) Observe the growt...

Embodiment 2

[0056] The preparation method of Phomopsis sp.YE3350 fermentation crude extract comprises the following steps:

[0057] (1) Activation of the bacterial strain: Take out the test tube stored in the bacterial strain Phomopsis sp.YE3350, pick a small amount of hyphae with a bamboo stick under aseptic conditions, inoculate it into a sterilized PDA medium test tube, and put it in the incubator. Cultivate at 28±2°C for 7 days to obtain activated strains.

[0058] (2) Preparation of PDB medium: Weigh 200g of peeled potatoes, cut into small pieces, add 1000mL of distilled water to boil, filter with gauze to obtain potato filtrate, add 20g of glucose, keep the pH natural, and sterilize at 121°C for 30min to prepare PDB medium Standby, wherein the concentration of potato in the PDB medium is 0.2g / mL, and the concentration of glucose is 0.02g / mL.

[0059] (3) Preparation of seed liquid: under aseptic conditions, pick a small amount of mycelium of the activated strain with a bamboo stick...

Embodiment 3

[0063] Determination of antipathogenic activity and in vitro antioxidant capacity of the fermented crude extract of strain Phomopsis sp.YE3350:

[0064] (1) Determination of antibacterial activity:

[0065] The 8 pathogenic indicator bacteria used included 3 human pathogenic bacteria: Staphylococcus aureus, Salmonella typhimurium, methicillin-resistant Staphylococcus aureus (MRSA); 1 human pathogenic fungus : Candida albicans and 4 plant pathogenic fungi: Fusarium solani, Glomerella cingulata, Gibberella saubinetii and Botrytis cinerea. The fungal medium is PDA medium; the bacterial medium is LB solid medium.

[0066] The growth inhibitory activity of the fermented crude extract of strain Phomopsis sp.YE3350 on 8 kinds of pathogenic microorganisms was determined by the filter paper diffusion method, and the diameter of the filter paper was 7mm. Under sterile conditions, draw 0.2mL of the suspension of the indicator bacteria to be tested, add the corresponding solid medium to...

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Abstract

The invention provides a novel phomopsis fungus strain and belongs to the technical field of microorganisms. The novel phomopsis fungus strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO. M 2020394. The novel phomopsis strain has broad-spectrum antibacterial activity and antioxidation activity and can be applied to preparation of agricultural or medical microbial antibacterial agents and antioxidants. The invention further provides a preparation method and application of the novel phomopsis fungus strain.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new Phomopsis fungus strain, a preparation method and an application. Background technique [0002] Endophytes are fungi or bacteria that live in the tissues and organs of healthy plants in certain stages or all stages. Ubiquitously present in higher plants, endophytic bacteria are found in woody, herbaceous, monocotyledonous and dicotyledonous plants. At present, plant endophytes have become potential microbial pesticides, production-increasing bacteria or potential bio-control carrier bacteria in biological control. [0003] Gmelina arborea, a national medicinal plant, is a semi-deciduous tree of the Verbenaceae Gmelina genus. It is a medicinal and edible plant of the Dai nationality. It is also a precious fast-growing timber tree species. higher. The wood of Yunnan Shizi has excellent antiseptic properties, and it is also an important medicinal plant in Dai and other...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00A61K36/06A61P31/04A61P31/10A61P39/06C12R1/645
CPCC12N1/14A01N63/30A61K36/06A61P31/04A61P31/10A61P39/06Y02A50/30
Inventor 吴少华鲁祎晗刘思思
Owner YUNNAN UNIV
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