Rapid blood fat detector and detection method

A detection method and technology of detectors, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of long color reaction time, errors, and many influencing factors, and achieve the effect of high-sensitivity and rapid detection

Pending Publication Date: 2021-11-05
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of blood lipid meter generally has the following disadvantages: 1. The color reaction time is long, and there are many influencing factors, which easily lead to large errors; 2. The measurement of reflected light intensity is easily affected by the color uniformity of the test strip, base reflection and light scattering. 3. It must include light source, light intensity detector and optical path instrument, and it is difficult to miniaturize the instrument
[0004] This inherent deficiency in design is caused by the use of colorimetric reflectance photometry, and it is difficult to make up for it through technological improvements.

Method used

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  • Rapid blood fat detector and detection method

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Experimental program
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Effect test

Embodiment 1

[0040] A rapid blood lipid detector provided in this embodiment includes a test strip 1, an electrode plate 4, and an electrochemical workstation 5, the test strip is provided with a siphon port 2 for absorbing the solution to be tested, and the siphon port The volume is preferably 0.0012cm 3 , the test strip is connected to the electrode plate through the three-electrode system 3, and the electrode plate 4 is connected to the electrochemical workstation 5 for measurement;

[0041] There are three kinds of test strips 1, which are test strips immobilized with cholesterol oxidase for the determination of total cholesterol, test strips immobilized with cholesterol esterase for the determination of high-density lipoprotein cholesterol, and immobilized with lipoprotein fatty acid and glycerol kinase. Test strips for the determination of triglycerides.

[0042] Described three-electrode system 3 comprises the working electrode that is arranged on test strip successively, reference...

Embodiment 2

[0044] This embodiment provides a method for rapid detection of blood lipids, using the rapid detection instrument for blood lipids as described in Example 1, which specifically includes the following steps:

[0045] Step S1: Determination of total cholesterol (TC): place the test strip for the determination of total cholesterol in the solution to be tested, suck the solution to be tested through the siphon effect of the siphon port, and then place the working electrode of the test strip for the determination of total cholesterol, The reference electrode and the counter electrode are respectively inserted into the three interfaces of the electrode plate, and connected to the chemical workstation for measurement;

[0046] Determination of triglyceride (TG): Place the test paper strip for triglyceride determination in the solution to be tested, and after inhaling the solution to be tested through the siphon effect of the siphon port, put the working electrode of the test paper stri...

Embodiment 3

[0060] Study on Initial Stability of Test Strips

[0061] Stability and reproducibility experiments were carried out on the test strips in the initial state without any enzyme immobilized. 5.0mmol / L potassium ferricyanide was used as the measuring probe, differential pulse voltammetry was used as the measuring method, and the scanning potential was -0.2 ~0.6V, the scan rate is 100mV, the oxidation peak is as figure 2 It can be seen from the figure that. The screen-printed electrodes showed good stability and reproducibility. 20 sets of test strips were further studied and their peak current data were analyzed. The results are shown in Table 1:

[0062] Table 1 Stability test data of printed electrodes

[0063]

[0064] The relative deviation of oxidation peak current value measured by 20 groups of test strips is 0.20%, which shows that the customized test strips have good stability.

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Abstract

The invention discloses a rapid blood fat detector and a detection method, the rapid blood fat detector comprises a test strip, an electrode plate and an electrochemical workstation, the test strip is provided with a siphon port for absorbing a solution to be detected, the test strip is connected with the electrode plate through a three-electrode system, and the electrode plate is connected with the electrochemical workstation for determination; the test strip comprises three types, namely a test strip for determining total cholesterol fixed with cholesterol oxidase, a test strip for determining high-density lipoprotein cholesterol fixed with cholesterol esterase and a test strip for determining triglyceride fixed with lipoprotein fatty acid and glycerol kinase, and by adopting a screen printing test strip, utilizing an electrochemical method and combining the catalytic action of enzyme on a substrate, high-sensitivity rapid detection of TG, TC and HDL-C values can be realized, then the LDL-C value is obtained through calculation, and rapid detection of four items of blood fat is realized.

Description

technical field [0001] The invention relates to the technical field of blood lipid inspection, in particular to a rapid blood lipid detector and a detection method. Background technique [0002] Blood lipid testing includes total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and other items. The number of adults with dyslipidemia in my country has reached 180 million. Hyperlipidemia can lead to stroke, coronary heart disease, myocardial infarction and sudden death. Therefore, regular blood lipid testing is of great significance for the prevention and treatment of cardiovascular and cerebrovascular diseases. [0003] At present, the principle of the rapid blood lipid detector on the market is to use the dry chemical enzyme method, the collected blood reacts with the chemical reagents and enzymes in the test strip for biochemical color development, and the blood lipid meter detects the reflected light intensity of the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/53
CPCG01N27/3272G01N33/53
Inventor 张连明李旦李建平潘宏程罗奎高静霞孔苏苏
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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