Rapid blood fat detector and detection method
A detection method and technology of detectors, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of long color reaction time, errors, and many influencing factors, and achieve the effect of high-sensitivity and rapid detection
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Embodiment 1
[0040] A rapid blood lipid detector provided in this embodiment includes a test strip 1, an electrode plate 4, and an electrochemical workstation 5, the test strip is provided with a siphon port 2 for absorbing the solution to be tested, and the siphon port The volume is preferably 0.0012cm 3 , the test strip is connected to the electrode plate through the three-electrode system 3, and the electrode plate 4 is connected to the electrochemical workstation 5 for measurement;
[0041] There are three kinds of test strips 1, which are test strips immobilized with cholesterol oxidase for the determination of total cholesterol, test strips immobilized with cholesterol esterase for the determination of high-density lipoprotein cholesterol, and immobilized with lipoprotein fatty acid and glycerol kinase. Test strips for the determination of triglycerides.
[0042] Described three-electrode system 3 comprises the working electrode that is arranged on test strip successively, reference...
Embodiment 2
[0044] This embodiment provides a method for rapid detection of blood lipids, using the rapid detection instrument for blood lipids as described in Example 1, which specifically includes the following steps:
[0045] Step S1: Determination of total cholesterol (TC): place the test strip for the determination of total cholesterol in the solution to be tested, suck the solution to be tested through the siphon effect of the siphon port, and then place the working electrode of the test strip for the determination of total cholesterol, The reference electrode and the counter electrode are respectively inserted into the three interfaces of the electrode plate, and connected to the chemical workstation for measurement;
[0046] Determination of triglyceride (TG): Place the test paper strip for triglyceride determination in the solution to be tested, and after inhaling the solution to be tested through the siphon effect of the siphon port, put the working electrode of the test paper stri...
Embodiment 3
[0060] Study on Initial Stability of Test Strips
[0061] Stability and reproducibility experiments were carried out on the test strips in the initial state without any enzyme immobilized. 5.0mmol / L potassium ferricyanide was used as the measuring probe, differential pulse voltammetry was used as the measuring method, and the scanning potential was -0.2 ~0.6V, the scan rate is 100mV, the oxidation peak is as figure 2 It can be seen from the figure that. The screen-printed electrodes showed good stability and reproducibility. 20 sets of test strips were further studied and their peak current data were analyzed. The results are shown in Table 1:
[0062] Table 1 Stability test data of printed electrodes
[0063]
[0064] The relative deviation of oxidation peak current value measured by 20 groups of test strips is 0.20%, which shows that the customized test strips have good stability.
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