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Application of insoluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis

A technology of soluble dietary fiber and ulcerative colitis, applied in the application field of medicine and functional food, can solve the problems such as the inability of the kelp industry to develop and grow, environmental pollution, waste of resources, etc., to alleviate the histopathological condition, and to have rich sources. , enhance the effect of hydrophilic

Active Publication Date: 2021-12-03
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The waste residue of kelp contains a large amount of dietary fiber, which not only causes waste of resources, but also causes environmental pollution, making the kelp industry unable to develop and grow

Method used

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  • Application of insoluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis
  • Application of insoluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis
  • Application of insoluble dietary fiber in kelp in preparation of medicine and functional food for improving ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Extraction of insoluble dietary fiber

[0048] The extraction method of insoluble dietary fiber among the present invention comprises the following steps:

[0049] (1) Add 5% NaCl solution to the kelp residue according to the ratio of solid to liquid ratio of 1:30, and heat at 90°C for 7 h for decalcification;

[0050] (2) Centrifuge the sample in step (1), collect the precipitate, add an appropriate amount of pure water to wash, and collect the precipitate again;

[0051] (3) Add 5 mol / L NaOH solution to the precipitate in step (2) according to the ratio of solid to liquid ratio of 1:30, adjust the pH to alkaline, and heat at 90°C for 6 h;

[0052](4) Centrifuge the sample in step (3), and collect the precipitate as insoluble dietary fiber (IDF);

[0053] (5) Add NaClO with a volume fraction of 9% to the precipitate in step (4) according to the liquid-to-solid ratio of 12:1, and decolorize the IDF for 67 minutes; the whiteness of IDF after bleaching is ...

Embodiment 2

[0058] Example 2: Identification of the basic structure of kelp insoluble dietary fiber (IDF)

[0059] 1. Determination of the monosaccharide composition of IDF by high performance liquid chromatography

[0060] (1) Accurately weigh 2 mg of IDF prepared in Example 1, add 400 μL of 2 mol / L trifluoroacetic acid (TFA) solution into the ampoule, and degrade under sealed conditions at 105°C for 6 h. After the degradation is completed, add methanol repeatedly to remove excess trifluoroacetic acid, and store the degradation product in a dry environment.

[0061] (2) 100 μL of distilled water fully dissolved IDF degradation products and monosaccharide standard, added 100 μL of 0.3 mol / L NaOH and 120 μL of 0.5 mol / L PMP methanol solution, and bathed in 70°C water bath for 60 min. After cooling to room temperature, 100 μL of 0.3 mol / L HCl solution was added for neutralization reaction, extracted three times with dichloromethane to remove unreacted PMP, and the supernatant was filtered ...

Embodiment 3

[0078] Select C57BL / 6 mice (male), 7-8 weeks old, body weight 25-28g, rearing temperature 20-24°C, humidity 40%-60%, free to drink water and eat during the period. The experimental grouping was as follows: 72 male C57BL / 6 mice were adaptively fed for 7 days and randomly divided into 6 groups according to body weight, 12 in each group, which were normal group (NC), model group (MC), positive drug group (PC), Low, medium and high dose IDF groups (L-IDF, M-IDF, H-IDF). At the end of adaptive feeding, the test substance was gavaged. The mice in each group were gavaged with the test substance every day at 9:00 am for 7 days. On the 8th day, dextran sodium sulfate (DSS) was used to establish the ulcerative colitis model. After the last meal, on the 14th day, the mice were fasted without food and water overnight. The next day, the mice were sacrificed by dislodging the cervical spine after taking blood from the eye socket. Intervention plan See Table 2. During the intervening mode...

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Abstract

The invention discloses an application of insoluble dietary fibers in kelp in preparation of a medicine and functional food for improving ulcerative colitis. According to the invention, insoluble dietary fiber is extracted from kelp residues, an ulcerative colitis mouse model is constructed, and it is verified for the first time that the insoluble dietary fibers can inhibit mouse weight loss caused by ulcerative colitis, inhibit colon lesion and shortening degree, improve an oxidative stress level of colon, balance a level of inflammatory factors in vivo, and up-regulate expression of intestinal tight junction protein. Therefore, the insoluble dietary fibers can be used as a prebiotic component for targeted regulation of intestinal flora and metabolites thereof, and can improve ulcerative colitis symptoms by mediating a plurality of intestinal metabolism signals.

Description

technical field [0001] The invention belongs to the field of food health care, and in particular relates to the application of insoluble dietary fiber in kelp in preparing medicine and functional food for improving ulcerative colitis. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) includes two subtypes of Crohn's disease (Crohn's disease, CD) and ulcerative colitis (Ulcerative Colitis, UC). Chronic relapsing disease with pathological characteristics has a high incidence in western countries and rising incidence in developing countries, and IBD patients are often accompanied by anxiety, depression and other mental diseases. [0003] As primary producers in the ocean, seaweed contains a variety of active substances. Among them, kelp dietary fiber has gradually become a research hotspot in the fields of medicine and food because of its wide range of biological activities. As a large seaweed, kelp contains almost no lignin, which mak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/03A61P1/04A61P1/00A61P29/00A23L33/21
CPCA61K36/03A61P1/04A61P1/00A61P29/00A23L33/21A23V2002/00A23V2200/3202A23V2250/5116Y02A50/30
Inventor 缪锦来曹峻菡曲长凤何英英郑洲李江张丽萍王凯王西西秦玲
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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