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New anti-saccharification application of litsea coreana and anti-saccharification product and preparation method of anti-saccharification product

An eagle tea, anti-glycation technology, applied to medical preparations containing active ingredients, dressing preparations, cosmetic preparations, etc.

Active Publication Date: 2021-12-21
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The reported effects of Eagle Tea involve lowering blood sugar, antioxidant activity, lowering blood lipids, quenching thirst and heat, eliminating food and swelling, detoxifying and reducing swelling, refreshing and improving intelligence, improving eyesight and stomach, dissipating blood stasis and relieving pain, stopping diarrhea, and stopping hiccups, etc. However, there are no reports in the art about the use of eagle tea in anti-glycation (inhibition of fructosamine, carbonylation, AGEs, protein cross-linking)

Method used

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  • New anti-saccharification application of litsea coreana and anti-saccharification product and preparation method of anti-saccharification product
  • New anti-saccharification application of litsea coreana and anti-saccharification product and preparation method of anti-saccharification product
  • New anti-saccharification application of litsea coreana and anti-saccharification product and preparation method of anti-saccharification product

Examples

Experimental program
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Effect test

preparation example Construction

[0077] The embodiment of this group provides a preparation method of an anti-glycation product. All the examples in this group have the following common features: the extract of eagle tea is used as the anti-glycation active ingredient of anti-glycation products.

[0078] In a specific embodiment, the eagle tea extract refers to the product obtained by extracting eagle tea in water at high temperature;

[0079] In some embodiments, preferably, the extraction temperature of the high-temperature extraction is 50-100°C, preferably 80°C. The advantage of using this extraction temperature is to extract as much as possible without destroying the effective substances in the tea leaves. Active ingredients in tea leaves, and make the active ingredients extracted closer to the active ingredients that can be obtained by daily brewing tea.

[0080] The extraction time is 1h-24h, preferably 2h; the advantage of using this extraction time is to extract the active ingredients in the tea as ...

experiment example 1

[0084] Experimental Example 1, Extraction of Eagle Tea Active Substances

[0085] 1.1 Extraction of active substances in eagle tea

[0086] Since eagle tea is often used as tea drink, this experiment is mainly based on the method of drinking tea, improving and extracting the effective substances of eagle tea.

[0087] 1.1.1 Reagents and instruments

[0088] Electronic balance (TB-2002, Beijing Sartorius Instrument System Co., Ltd.); pulverizer (JP-400B-8); ultra-low temperature preservation box (DW-86L388A, Haier Group); freeze dryer (VIRTIS); rotary evaporator; Vacuum filter

[0089] Reagent: deionized water

[0090] 1.1.2 Experimental method

[0091] Pulverize the tea leaves with a pulverizer, pass through a 24-mesh sieve, and set aside. According to the ratio of material to liquid 1:50 (g / ml), weigh 4.0g of raw materials and 200ml of solvent (water), extract with distilled water (80°C, 2h), filter, take the filtrate, and use a rotary evaporator to dilute the filtrate (...

experiment example 2

[0120] Experimental example 2. In vitro non-enzymatic glycosylation inhibition experiment of eagle tea

[0121] 2.1 Main instruments and reagents

[0122] Multifunctional microplate reader (M200PRO, TECAN); analytical balance; pipette gun (200μL, 1mL, 5mL);

[0123] Bovine serum albumin (BSA); aminoguanidine sulfate hydrate (A0309, Tokyo Chemical Industry Co., Ltd.); sodium azide; fructose; Na 2 HPO 4 12H 2 O(AR, Sinopharm Chemical Reagent Co., Ltd.); NaH 2 PO 4 2H 2 O(AR, Sinopharm Chemical Reagent Co., Ltd.);

[0124] Prepare PBS with pH=7.3-7.4, and add 0.02% sodium azide to the PBS solution

[0125] 20mg / mL bovine serum albumin solution (BSA solution): Weigh 0.5g BSA, add PBS to 25mL, filter with 0.45μm water filter membrane:

[0126] 0.5mol / L fructose solution: weigh 2.25g fructose, and dilute to 25mL with PBS;

[0127] BSA-fructose reaction solution: Mix 20 mL of BSA solution (20 mg / mL) and 20 mL of fructose solution (0.5 mol / L).

[0128] 2.2 In vitro non-enzym...

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Abstract

The invention relates to a new anti-saccharification application of litsea coreana, an anti-saccharification product and a preparation method of the anti-saccharification product, and belongs to the technical field of plant extraction. On one hand, the invention provides the new anti-saccharification application of the litsea coreana. On the other hand, the invention provides the anti-saccharification product which is characterized by comprising an anti-saccharification active component; the action target of the anti-saccharification active component is selected from a group consisting of fructosamine, a protein carbonylation reaction pathway, AGEs, and a reaction pathway for crosslinking of a non-enzymatic glycosylation reaction intermediate product and protein; and the anti-saccharification active component comprises a litsea coreana extract. The third aspect of the invention provides a preparation method of the anti-saccharification product, which is characterized in that the litsea coreana extract is used as the anti-saccharification active component of the anti-saccharification product. Experiments prove that the litsea coreana extract has more excellent effects on inhibition of fructosamine, protein carbonylation and protein crosslinking than positive control aminoguanidine.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a new anti-glycation application of eagle tea, an anti-glycation product and a preparation method thereof. Background technique [0002] Eagle tea LitseacoreanaLevlvar lanuginose (Migo) Yang et P.H.Huang is the tea obtained after drying the twigs and leaves of Leopard camphor. It is a specialty of Shimian County, Sichuan Province, and a national geographical indication product of China. The locals call it eagle tea, also known as old tea. The leaves of eagle tea are oval, with green face and white back, so it is also called white tea. Eagle tea contains many ingredients, such as amino acids, mineral elements, vitamin C, vitamin B1, vitamin B2, flavonoids and polyphenols. The high iron, high selenium, high zinc, and high chromium features are beneficial to improve the functions of the hematopoietic system and endocrine system; other trace elements such as rub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61K36/54A61P17/18A23L33/10
CPCA61K8/9789A61Q19/08A61K36/54A61P17/18A23L33/10A61K2236/331A61K2236/51A61K2800/84A23V2002/00A23V2200/30
Inventor 易帆李丽董银卯何一凡陈春宇
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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