Nanofiber membrane for skin wound and preparation method thereof

A nanofibrous membrane and nanozyme technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve problems such as inability to effectively inhibit scars, and achieve the effect of promoting rapid healing, inhibiting wound inflammation, and inhibiting tissue inflammation.

Active Publication Date: 2022-01-04
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a nanofibrous membrane for skin wounds and its preparation method, which overcomes the defect that the prior art cannot effectively suppress scars

Method used

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  • Nanofiber membrane for skin wound and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) The preparation by solvothermal reaction is as follows: respectively configure ferric chloride solution and zinc acetate solution with a concentration of 5mM, and then mix 1mL with 2mM polyvinylpyrrolidone of 28mg / mL, and take 2mL of 5mM polyvinylpyrrolidone K 4 [Fe(CN) 6 ] solution was slowly added to the above mixed solution, and reacted for 10 minutes at a temperature of 50° C. to obtain Prussian blue analogues (PBAs). Wash by centrifugation at a rotational speed of 20,000 r / min, and then freeze-dry to obtain the PBAs powder product.

[0030] (2) Dissolve 10mL of 5nM PBAs and 10mL of 5uM AOND in 25°C and stir for 8 hours, then centrifuge and wash with deionized water at a speed of 20000r / min, freeze-dry, and obtain the PBAs / AOND nano Enzyme composites. (3) Gelatin is dissolved in hexafluoroisopropanol to obtain a gelatin solution with a mass fraction of 8% as a shell solution; polycaprolactone is dissolved in hexafluoroisopropanol to obtain a mass fraction of ...

Embodiment 2

[0033] (1) Preparation by solvothermal reaction is specifically: ferric chloride and zinc acetate at a molar ratio of 2:1, both at a concentration of 8mM, taking 2mL and 1mL respectively, and mixing them with 3mL of 28mg / mL polyvinylpyrrolidone evenly, taking 3 mL of 5 mM K 4 [Fe(CN) 6 ] solution was slowly added to the above mixed solution, and reacted for 30 minutes at a temperature of 70° C. to obtain Prussian blue analogues (PBAs). Wash by centrifugation at a rotational speed of 15000r / min, and then freeze-dry to obtain the PBAs powder product.

[0034] (2) Dissolve 10mL of 5nM PBAs and 10mL of 5uM AOND obtained in step a and stir at 25°C for 10 hours, then centrifuge and wash with deionized water at a speed of 15000r / min, and freeze-dry to obtain the PBAs / AOND nanozyme composite material.

[0035] (3) Gelatin is dissolved in hexafluoroisopropanol to obtain a gelatin solution with a mass fraction of 12% as a shell solution; polycaprolactone is dissolved in hexafluoroi...

Embodiment 3

[0038](1) Preparation by solvothermal reaction is specifically: ferric chloride, zinc acetate, the concentration is 5mM, each take 1mL, and mix with 28mg / mL polyvinylpyrrolidone 3mL, take 2mL of 5mM concentration of K 4 [Fe(CN) 6 ] solution was slowly added to the above mixed solution, and reacted for 30 minutes at a temperature of 70° C. to obtain Prussian blue analogues (PBAs). Wash by centrifugation at a rotational speed of 15000r / min, and then freeze-dry to obtain the PBAs powder product.

[0039] (2) Gelatin is dissolved in hexafluoroisopropanol to obtain a gelatin solution with a mass fraction of 8% as a shell solution; polycaprolactone is dissolved in hexafluoroisopropanol to obtain a mass fraction of 10% polycaprolactone solution, and then 0.1 mg of PBANPs was added to obtain a nuclear solution; under the conditions of a temperature of 20° C. and a humidity of 40%, the nanofibrous membrane was prepared under the regulated spinning process. Co-spun to obtain PCL / AOND-...

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Abstract

The invention relates to a nanofiber membrane for a skin wound and a preparation method thereof. The nanofiber membrane is of a core-shell structure, wherein a shell layer material contains gelatin; a core layer material contains a polycaprolactone and nano-enzyme composite material. According to the invention, wound inflammation is inhibited by using the activity of PBAs nano enzyme, hydrogen peroxide and superoxide enzyme, and antisense oligonucleotide AOND selectively inhibits expression of TGF-beta1, thus inhibiting formation of a scar and promoting healing of the wound.

Description

technical field [0001] The invention belongs to the field of functional nanofiber membrane and its preparation, in particular to a nanofiber membrane for skin wounds and a preparation method thereof. Background technique [0002] Signaling pathways are activated during wound healing that work together to restore the tissue microenvironment consisting of cells and extracellular matrix (ECM), including enzymes, cytokines, and growth factors. The process of wound healing includes four phases: coagulation phase, inflammation phase, repair phase and remodeling phase. Reactive oxygen species (ROS), such as hydrogen peroxide, hydroxyl radicals, and superoxide radicals, are excessively produced at the damaged site. Although ROS have important biological functions in the body, excessive reactive oxygen species affect cell proliferation and lead to apoptosis through oxidative damage to cell membranes, DNA, and proteins, while unresolved inflammation is the main cause of impaired woun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/18A61L15/20A61L15/26A61L15/44B82Y5/00
CPCA61L15/32A61L15/26A61L15/18A61L15/20A61L15/44B82Y5/00A61L2300/102A61L2300/412A61L2400/12C08L89/00C08L67/04
Inventor 张迎章一新陈云生张铮
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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