Metal-containing efficient cationic antibacterial polymer as well as preparation method and application thereof
A technology of metal cations and polymers, applied in the field of antibacterial materials, can solve the problems that the sterilization efficiency needs to be enhanced and the selectivity of sterilization needs to be improved, and achieve the effect of broad-spectrum antibacterial properties and simple synthesis process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] Embodiment 1: the synthesis of metal cationic antibacterial polymer
[0045] The chemical reaction process of synthesizing metal cationic antibacterial polymer is as follows: figure 1 shown. Specifically, trimethylolpropane allyl ether (3.75g, 0.02mol, Sinopharm), anhydrous pyridine (3.45g, 0.44mol, Sinopharm) and anhydrous dichloromethane (30mL, Sinopharm) were added to nitrogen protection In the there-necked flask, the adipoyl chloride (Aladdin) of 3.94g is joined in the flask through the constant pressure dropping funnel, and reacted at room temperature for 48 hours. Pour the reaction solution into a large amount of methanol solution to precipitate and dry to obtain a high molecular weight polyester with double bond functional groups.
[0046] Take 2.0 g of the above polyester and 2.0 g of m-chloroperoxybenzoic acid (Aladdin), add them into 20 mL of anhydrous DMF, and react at room temperature for 12 hours under nitrogen protection. Precipitate and dry the reactio...
Embodiment 2
[0049] Embodiment 2: the bactericidal performance of metal cationic antibacterial polymer
[0050] The bactericidal properties of metal cationic antimicrobial polymers were measured by plate bactericidal experiments. Use Tris buffer (10mM Tris, 142mM NaCl, pH 7.4) as the buffer solution, use Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Bacillus subtilis (ATCC6051), Staphylococcus aureus (ATCC25923) as the experimental bacteria kind. Cultivate the bacteria to be used to the logarithmic growth phase by the second inoculation method, then centrifuge the bacteria solution to remove the supernatant, wash it twice with buffer solution, and then dilute the bacteria to 1.5×10 6 CFU / mL concentration is ready for use. Different concentrations of metal cationic antibacterial polymers (Formula II) were dissolved in PBS. Take 50 microliters of antibacterial polymer solution and 100 microliters of bacteria solution and shake at room temperature for 3 hours. Take 20 ...
Embodiment 3
[0051] Embodiment 3: the bacteriostatic performance of metal cationic antibacterial polymer
[0052] The antimicrobial properties of metal-cationic antimicrobial polymers were determined by the trace broth method. At first according to the method similar to Example 1 (the difference is that zinc nitrate is replaced by nickel nitrate and cobalt nitrate) to prepare the metal cationic antibacterial polymer containing cobalt and nickel. A series of metal cationic polymer solutions with different concentrations were prepared by dilution method with MH medium for use. Before the experiment, the bacteria to be tested were cultured in the medium to the logarithmic growth phase by the method of secondary inoculation, then the bacterial liquid was centrifuged to remove the supernatant, washed twice with Tris buffer solution, and then diluted with MH medium to 1.5×10 6 CFU / mL concentration is ready for use. Take 50 microliters of polymer solution and 100 microliters of bacteria soluti...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More