Cell-derived fluorescent carbon nanosheet as well as preparation method and application thereof

A carbon nanosheet and fluorescence technology, applied in nanocarbon, nanotechnology, nanotechnology and other directions, can solve the problems affecting the application of two-dimensional fluorescent carbon nanomaterials, and achieve good photothermal conversion ability, good chemical composition, and fast growth speed. Effect

Active Publication Date: 2022-02-01
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the two-dimensional fluorescent carbon nanomaterials prepared by these methods will introduce exogenous carbon nanomaterials in biological applications, and their toxicity and long-term effects on organisms still affect the further application of these two-dimensional fluorescent carbon nanomaterials.

Method used

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  • Cell-derived fluorescent carbon nanosheet as well as preparation method and application thereof
  • Cell-derived fluorescent carbon nanosheet as well as preparation method and application thereof
  • Cell-derived fluorescent carbon nanosheet as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0055] (1) Absorb the culture medium from the adherent HeLa tumor cells grown in logarithmic phase in DMEM medium, wash with PBS (pH7.2) three times, add trypsin-EDTA and let stand for 5min, observe the cells slowly under the microscope After becoming round to remove the adherent state, add DMEM medium equal to trypsin-EDTA and mix thoroughly. After mixing, centrifuge at 1000rpm for 5 minutes. Remove the supernatant and mix the precipitate with deionized water at a mass ratio of 1:5.

[0056] (2) After the above solution is transferred to the reactor, put it into a high-temperature oven, react at 180° C. for 22 hours, stop the reaction, wait for the reactor to be slowly cooled to room temperature, take out the solution from the reactor and perform suction filtration, and remove the filter residue. An aqueous solution of fluorescent carbon nanosheets is obtained.

[0057] (3) freeze-drying the aqueous solution of fluorescent carbon nanosheets obtained in step (2) to obtain soli...

Embodiment 2

[0059] (1) The adherent MCF-7 tumor cells grown in logarithmic phase cultured in the medium were sucked off the medium, washed three times with PBS (pH7.2), added trypsin-EDTA and left for 5 minutes, and observed under the microscope that the cells slowed down. After slowly becoming round to remove the adherent state, add DMEM medium equal to the amount of trypsin-EDTA and mix well. After mixing, centrifuge at 1000rpm for 5 minutes. After removing the supernatant, the precipitate and deionized water are uniform at a mass ratio of 1:10.

[0060] (2) After the above solution is transferred to the reactor, put it into a high-temperature oven, react at 160° C. for 24 hours, stop the reaction, wait for the reactor to be slowly cooled to room temperature, take out the solution from the reactor and perform suction filtration, and remove the filter residue. An aqueous solution of fluorescent carbon nanosheets is obtained.

[0061] (3) freeze-drying the aqueous solution of fluorescent ...

Embodiment 3

[0063] (1) The adherent HepG2 tumor cells grown in logarithmic phase cultured in the medium were sucked off the medium, washed three times with PBS (pH 7.2), added trypsin-EDTA and left for 5 minutes, and the cells slowly changed under a microscope. After removing the adherent state, add DMEM medium equal to trypsin-EDTA and mix well. After mixing, centrifuge at 1000rpm for 5 minutes. After removing the supernatant, the precipitate and deionized water are evenly mixed at a mass ratio of 1:10.

[0064] (2) After the above solution is transferred to the reaction kettle, put it into a high-temperature oven, react at 200°C for 20h, stop the reaction, wait for the reaction kettle to be slowly cooled to room temperature, take out the solution from the reaction kettle and carry out suction filtration, and remove the filter residue, An aqueous solution of fluorescent carbon nanosheets is obtained.

[0065] (3) freeze-drying the aqueous solution of fluorescent carbon nanosheets obtaine...

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Abstract

The invention discloses a cell-derived fluorescent carbon nanosheet as well as a preparation method and an application thereof. The cell-derived fluorescent carbon nanosheet is formed by carrying out hydrothermal reaction on tumor cells and deionized water. The synthesized cell-derived fluorescent carbon nanosheet has the advantages of high fluorescence and quantum yield, uniform morphology, good fluorescence stability, good biocompatibility and good photo-thermal performance; meanwhile, the preparation method is simple and convenient, wide in raw material source, low in cost and suitable for industrial production and application. By increasing the excitation wavelength, the cell-derived fluorescent carbon nanosheet prepared by the invention has the advantages of obvious red shift phenomenon, good excitation wavelength dependent fluorescence spectrum property and the like. Based on various excellent characteristics, the product can be prepared to serve as a fluorescent marker to be applied to cell imaging, and due to the good photo-thermal conversion efficiency, the product can be applied to photo-thermal therapy of organisms or cells.

Description

technical field [0001] The invention belongs to the field of chemical nanomaterials, and in particular relates to a cell-derived fluorescent carbon nanosheet, a preparation method thereof, and an application in cell imaging and tumor treatment. Background technique [0002] In recent years, non-metallic carbon-based nanomaterials such as graphene, carbon nanotubes, graphene quantum dots, carbon dots, etc. have been widely used in the field of cancer diagnosis and treatment due to their advantages such as low toxicity, good water solubility, low chemical activity and stable fluorescence properties. It shows a broad application prospect. Among them, the two-dimensional structure of carbon nanomaterials such as graphene is a single layer of carbon atoms with sp 2 The hexagonal honeycomb planar film composed of hybrid orbitals, whose valence band and conduction band intersect at the Fermi level, is a semiconductor with zero energy gap. However, as massless Dirac particles, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15A61K41/00A61K47/04A61K49/00A61P35/00B82Y20/00B82Y40/00
CPCC09K11/65C01B32/15A61K41/0052A61K47/02A61P35/00A61K49/0067B82Y20/00B82Y40/00Y02A50/30
Inventor 郑芬芬蒋迪飞潘一璠蒋恺刘文静谢居豪史竞成
Owner JIANGSU UNIV OF SCI & TECH
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