Bacillus patriniae and application thereof in prawn culture and medicine preparation
A technology of Bacillus and Bacillus, which is applied to the tomb painting Bacillus oceanic and its application in shrimp farming and preparation of medicines, can solve the problems of reduced resistance, easy disease and even death, unable to fully promote the growth of shrimp and other problems , to achieve the effect of inhibiting toxicity, good probiotics, and improving the activity of immune enzymes
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Embodiment 1
[0030] The preparation of each experimental group feed of embodiment 1
[0031] Experimental group 1 (group B5): Inoculate Bacillus oceanicus CGMCC No.19846 isolated and screened from the intestinal tract of Litopenaeus vannamei in 2216E liquid medium, culture in a constant temperature shaker at 28°C for 24 hours, and centrifuge at 4000rpm for 15 minutes to collect the bacteria Body, use sterile saline to resuspend the bacteria into a bacterial suspension. The bacterial suspension was evenly mixed with the extruded feed of Litopenaeus vannamei, so that the concentration of Bacillus oceanicus CGMCC No.19846 in the feed was 2×10 8 cfu / g, then heated and dissolved sodium alginate in distilled water (5.0-7.0g / kg) and mixed with fish oil (7.0-9.0mL / kg) as a protective agent, wrapped on the surface of the feed. After drying in the shade, store at 4°C for later use.
[0032]Experimental group 2 (BS group): Bacillus subtilis CCTCC AB 2010353 (from China Center for Type Culture Colle...
Embodiment 2
[0038] Example 2 Isolation and Identification of Oceanic Bacillus in Tomb Painting
[0039] 1. Isolation and purification of Bacillus oceanicus in the tomb painting: The samples were collected in Jindian Aquaculture Farm, Zhuanghe City, Dalian City. The characteristics of sample collection are as follows. Intestinal tract of Litopenaeus vannamei: body weight 12.37±0.18g, body length 11.06±2.19cm Vannamei healthy Litopenaeus vannamei, healthy Litopenaeus vannamei were dissected under aseptic conditions, and 5-10 prawns were used as a group to extract the whole intestines of the prawns in a sterile centrifuge tube and stored at 4°C for later use; Add 1mL sterile normal saline to a sterile mortar and thoroughly grind the homogenate, then transfer the homogenate to a sterile centrifuge tube. Put the above samples in a water bath at 80°C for 20±5min, inoculate them into 2216E liquid medium for enrichment culture at 28°C for 12-18h, and then use sterile water to carry out 10 -1 -10...
Embodiment 3
[0041] Example 3 Verification of Vibrio parahaemolyticus injection challenge test of Litopenaeus vannamei
[0042] Vibrio parahaemolyticus challenge test method was used to detect the disease resistance of Litopenaeus vannamei. In the challenge test, Vibrio parahaemolyticus (V.parahaemolyticus, sourced from China General Microorganism Culture Collection and Management Center, CGMCC 1.1997) was inoculated in 2216E liquid medium, cultured on a constant temperature shaker at 28°C for 24h, centrifuged at 4°C at 4000rpm for 10min, and collected Bacteria were diluted with sterile saline to a concentration of 5.6×10 5 The bacterial suspension of cfu / mL is ready for use. Forty-eight prawns of uniform size were randomly selected from each group of B5, BS, OP and CK and placed in a 60L white culture bucket, with 3 replicates in each group, and starved for 24 hours. Vibrio parahaemolyticus was injected into the second abdominal muscle of the prawns, and each tail was injected with 25 μ...
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