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Preparation method of lignin-based carbon nano enzyme with enzyme-like catalytic activity

A lignin-based, catalytically active technology, applied in the direction of catalyst activation/preparation, chemical instruments and methods, physical/chemical process catalysts, etc., can solve the problems of high raw material costs, hidden dangers of experimental safety, cumbersome synthesis steps, etc., and achieve synthetic methods Simple, wide source of raw materials, strong water solubility

Active Publication Date: 2022-02-25
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of high raw material cost, cumbersome synthesis steps, and potential safety hazards in the preparation process of nanozymes, and proposes a green, environmentally friendly, simple synthesis, easy to operate and large-scale synthesis, which combines low-value Preparation method of converting lignin into high-value carbon nanozyme with enzyme-like catalytic activity

Method used

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  • Preparation method of lignin-based carbon nano enzyme with enzyme-like catalytic activity
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  • Preparation method of lignin-based carbon nano enzyme with enzyme-like catalytic activity

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Embodiment 1

[0017] This embodiment is a preparation method of a lignin-based carbon nanozyme with enzyme-like catalytic activity, which is specifically completed according to the following steps:

[0018] 1. The preparation process of lignin-based carbon nanozyme: After adding 0.1g sodium lignosulfonate and 0.1g potassium chloride to 20mL ultrapure water, put the solution on a rapid mixer and mix evenly, then transfer the mixture into a stainless steel autoclave lined with polytetrafluoroethylene. Put the reaction kettle into an electric constant temperature blast drying oven, set the reaction time to 12h, and set the temperature to 180°C; after the reaction, take the reaction kettle out of the oven and cool it to room temperature;

[0019] 2. The purification process of lignin-based carbon nanozyme: put the mixture in the cooled reaction kettle in a centrifuge, set the rotation speed to 6000rpm, and the time is 10min. After centrifugation, pass the obtained supernatant through a 0.22μm m...

Embodiment 2

[0021] This embodiment is a detection process of enzyme-like catalytic activity of lignin-based carbon nanozyme, which is specifically completed according to the following steps:

[0022]Set up a system of 50 μL 3 mg / mL lignin-based carbon nanozyme aqueous solution, 50 μL 5 mmol / L hydrogen peroxide solution, and 50 μL 10 mmol / L 3`3`5`5`-tetramethylbenzidine solution and use pH=4 acetic acid -Sodium acetate buffer (0.1mol / L) to dilute the system by 2mL; set up the following two control groups at the same time: 50μL of 5mmol / L hydrogen peroxide was mixed with acetic acid-sodium acetate buffer (0.1mol / L) of pH=4 Solution, 50μL 10mmol / L 3`3`5`5`-tetramethylbenzidine solution diluted to 2mL system and 50μL 3mg / mL lignin-based carbon nanozyme aqueous solution, 50μL 10mmol / L 3`3`5`5`-tetramethylbenzidine solution diluted to 2mL system. After incubating the above three groups of solutions in the dark at room temperature for 10 minutes, the absorbance spectra of the solutions were rec...

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Abstract

The invention discloses a preparation method of lignin-based carbon nano-enzyme with enzyme-like catalytic activity, and relates to a preparation process for preparing nano-enzyme with peroxidase-like activity by taking lignin as a raw material. The invention aims to solve the problems of high preparation cost, complex synthesis method and the like in the conventional nano-enzyme preparation method. The preparation method comprises the following steps: taking sodium lignin sulfonate as a carbon source and potassium chloride as a doping agent, and synthesizing the lignin-based carbon nano-enzyme through a hydrothermal method. The peroxidase-like enzyme of the lignin-based carbon nano enzyme prepared by the method has high affinity to hydrogen peroxide, is low in cost and environment-friendly, the preparation method is simple, and high-value utilization of lignin is realized.

Description

technical field [0001] The invention relates to a method for preparing carbon nanozyme with peroxidase-like activity by using lignin as raw material. Background technique [0002] Lignin is the second largest resource of biomass material after cellulose in the plant world. The lignin produced by pulping, papermaking and biorefining in the world reaches more than 100 million tons every year. It has a wide range of sources and a large output, of which only about 10% is obtained. use efficiently. As a sustainable aromatic resource in nature, lignin is of great significance to the development of industrial agriculture. In industry, most of the recovered lignin is used for energy recovery, and only a small part is used for chemical production, reinforcing agent and mineral powder binder; in agriculture, lignin can be used to synthesize fertilizers through modification and processing. , soil loosening agent, pesticide slow-release agent, etc. At present, most lignin is directly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J21/18B01J35/00B01J37/00B01J37/10G01N21/31G01N21/78G01N31/10
CPCB01J21/18B01J37/10B01J37/009G01N31/10G01N21/78G01N21/31B01J35/23
Inventor 牛娜徐小雨陈立钢
Owner NORTHEAST FORESTRY UNIVERSITY
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