Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Viscous biological sample liquefaction release combination product, kit, liquefaction release method and nucleic acid extraction, amplification and detection method

A technology of biological samples and combined products, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of complex operation, insufficient, sample mixing and liquefaction, etc., and achieve good release effect and good compatibility Effect

Active Publication Date: 2022-03-01
SANSURE BIOTECH
View PDF43 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods generally have problems such as complex components, high cost, complicated operation, sample mixing and insufficient liquefaction, and how to efficiently release the nucleic acid in the liquefied sample is also a problem that needs to be solved in this field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Viscous biological sample liquefaction release combination product, kit, liquefaction release method and nucleic acid extraction, amplification and detection method
  • Viscous biological sample liquefaction release combination product, kit, liquefaction release method and nucleic acid extraction, amplification and detection method
  • Viscous biological sample liquefaction release combination product, kit, liquefaction release method and nucleic acid extraction, amplification and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0262] Embodiment 1. Liquefaction effect test

[0263] In this embodiment, the liquefaction reagent of the experimental group was prepared by using guaifenesin, sodium hydroxide and zirconia beads. The final concentration was 100mM guaifenesin, 10mM sodium hydroxide, and 1g / mL zirconia beads (diameter: 1mm). The solvent used to prepare the reagent was purified water, which was recorded as the experimental group.

[0264] Collected 4 cases of visually viscous sputum samples (I, II, III, IV), each of these sputum samples was fully mixed separately, and 6 2mL samples were taken from each case, numbered 1~6. spare.

[0265] Add 6 mL of experimental group reagent to sample No. 1;

[0266] Add 6 mL of dithiothreitol solution ((2% (w / v) DTT) to sample No. 2;

[0267] Add 6 mL of sodium hydroxide solution (0.1M) to sample No. 3;

[0268] Add 6mL of reagents prepared with a final concentration of 100mM acetylcysteine, 10mM sodium hydroxide, and 1g / mL zirconia beads (diameter: 1mm) ...

Embodiment 2

[0278] Example 2. Effect test of different concentrations of liquefied components combined with zirconia beads

[0279] In this embodiment, the liquefaction reagent of the experimental group consists of one or more of the following components: guaifenesin, sodium hydroxide, and zirconia beads.

[0280] Experimental group 1: The final concentration was 100mM guaifenesin, 10mM sodium hydroxide, 1g / mL zirconia beads (diameter 1mm), and the solvent used to prepare the reagent was purified water.

[0281] Experimental group 2: The final concentration was 1mM guaifenesin, 1000mM sodium hydroxide, 1g / mL zirconia beads (diameter 1mm), and the solvent used to prepare the reagent was purified water.

[0282] Experimental group 3: The final concentration was 1mM guaifenesin, 1g / mL zirconia beads (diameter: 1mm), and 0.1mM sodium hydroxide. The solvent used for preparing the reagent was purified water.

[0283] The collected 4 cases of viscous sputum samples (I, II, III, IV) were collect...

Embodiment 3

[0292] Example 3. Comparison with other methods for rapid detection of sputum samples after liquefaction

[0293] In this embodiment, the liquefaction reagent of the experimental group was prepared by using guaifenesin, sodium hydroxide and zirconia beads. The final concentration was 100mM guaifenesin, 10mM sodium hydroxide, and 1g / mL zirconia beads (1mm in diameter). The solvent used to prepare the reagent was purified water, which was used as the experimental group.

[0294] Collect 4 cases of visually viscous sputum samples (I, II, III, IV), mix each of these sputum samples fully separately, and take 5 2mL samples from each case, numbered 1~5. spare.

[0295] Add 6 mL of experimental group reagent to No. 1 sample; add 2.5 g of guanidine hydrochloride + 0.5 g of acetylcysteine ​​+ 2 g of polypropylene particles to No. 2 sample (the method disclosed in Patent Document No. CN108949748A); Add 6mL of dithiothreitol solution (2%DTT); add 6mL trypsin solution (buffer system cont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The way toaaaaaaaaaa
Login to View More

Abstract

The invention relates to a viscous biological sample liquefying and releasing combined product which comprises a liquefying component and a nucleic acid releasing component, wherein the liquefying component comprises guaifenesin and strong base; the nucleic acid release component comprises a component i) and / or a component ii), the component i) comprises 0.1%-2% of Tween 20, 0.1%-3% of Triton X-100, 0.1%-3% of ethyl phenyl polyethylene glycol, 20 mM to 1 M of Na < + > and / or K < + >, 50 mM to 1.25 M of strong base, an adsorbent and an aqueous solvent; and the component ii) comprises 0.01-0.5 mM of a surfactant, 0.01%-2% of dodecylbenzene sulfonate, 50-1.2 M of Na < + > and / or K < + >, 0.05%-1% of ethanol and a third strong base. The nucleic acid release component is matched with the liquefaction component for use, and has a better release effect on RNA and DNA.

Description

technical field [0001] The invention relates to the technical field of biological sample processing, in particular to a combination product for liquefaction and release of viscous biological samples. Background technique [0002] With the rapid development of technologies such as fluorescent quantitative PCR and multiplex PCR in the field of pathogen detection, there is an increasing demand for detecting whether the corresponding pathogen species is infected by the type of sputum sample. However, sputum samples are characterized by high viscosity, many proteins, and complex components, including mucin and other proteins (such as immune proteins), various enzymes, exfoliated cells, microorganisms, and other inhaled impurities, which are not convenient for direct detection. Clinically, the detection of sputum samples requires liquefaction of sputum. [0003] Common sputum liquefaction methods are sodium hydroxide method, DTT (dithiothreitol) method and protease method. The s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6806C12Q1/686
CPCC12Q1/6806C12Q1/686C12Q2527/125C12Q2523/308C12Q2563/107
Inventor 邓中平陈诗谣邓勇刘佳戴立忠
Owner SANSURE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com