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Method for detecting chlordimeform in aquatic products

A technology for dimeform and aquatic products, which is applied in the field of aquatic product safety testing, can solve problems such as inability to distinguish aromatic amine compounds, and achieve the effects of high accuracy of detection results, less time-consuming, and strong specificity

Active Publication Date: 2022-03-18
广东江门中医药职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The second method only needs to provide nitrite for diazotization to detect the target substance composition. Neither the conventional spectrophotometric method nor this detection method can distinguish aromatic amine compounds such as aniline, nitrobenzene, and methylamine in the sample. Interference from phosphorus etc.

Method used

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  • Method for detecting chlordimeform in aquatic products

Examples

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Embodiment 1

[0039] In this example, ethyl acetate was selected from analytically pure ethyl acetate supplied by Sinopharm Group Chemical Reagent Co., Ltd., anhydrous methanol was selected from analytically pure anhydrous methanol supplied by Sinopharm Group Chemical Reagent Co., Ltd., and sodium chloride was selected from Sinopharm Group Chemical Reagent Co., Ltd. For analytical pure sodium chloride and ammonium sulfate, choose analytical pure sodium sulfate supplied by Sinopharm Chemical Reagent Co., Ltd., and for calcium hydroxide, choose analytical pure calcium hydroxide supplied by Sinopharm Chemical Reagent Co., Ltd. For sodium hydroxide, choose Sinopharm Chemical Reagent Analytical pure sodium hydroxide supplied by Sinopharm Group Co., Ltd., sodium nitrite chooses analytically pure sodium nitrite supplied by Sinopharm Chemical Reagent Co., Ltd., and naphthaleneethylenediamine hydrochloride chooses analytically pure naphthaleneethylenediamine hydrochloride supplied by Sinopharm Chemica...

Embodiment 2

[0048] A method for detecting dimeform in aquatic products of the present invention comprises the following:

[0049] Step a: Weigh 3g±0.01g of the thawed homogeneous fish sample to be tested into the first 50ml centrifuge tube, add 4ml of ethyl acetate and 2ml of saturated sodium chloride solution (prepared at 20°C), shake for 3min , and then placed in a centrifuge at 10,000 rpm for 2 min to obtain a sample solution to be tested that is layered up and down;

[0050] Step b, draw 3ml ± 0.1ml of the supernatant of the sample solution to be tested into a second 5ml centrifuge tube, dry it with nitrogen, add 2ml of anhydrous methanol, fully shake for 1min, and redissolve to obtain the first sample solution;

[0051] Step c, transfer the first sample solution to a round-bottomed flask, add 20ml of sodium hydrosulfide solution with a mass fraction of 15%, and 5ml of 1mol / L ammonium sulfate solution, and heat at 40r / min under the condition of 180°C oil bath heating Stir at a consta...

Embodiment 3

[0058] A method for detecting dimeform in aquatic products of the present invention comprises the following:

[0059] Step a: Weigh 3g±0.01g of thawed homogeneous sample of shrimp meat to be tested in the first 50ml centrifuge tube, add 6ml of ethyl acetate and 4ml of saturated sodium chloride solution (prepared at 20°C), shake 3min, and then put it into a centrifuge at 10,000rpm for 2min to obtain a sample solution to be tested that is layered up and down;

[0060] Step b, draw 5ml ± 0.1ml of the supernatant of the sample solution to be tested into a second 10ml centrifuge tube, dry it with air, add 4ml of anhydrous methanol, fully shake for 1min, and redissolve to obtain the first sample solution;

[0061] Step c, transfer the first sample solution to a round-bottomed flask, add 30ml of sodium hydrosulfide solution with a mass fraction of 15%, 10ml of 1mol / L ammonium sulfate solution, and heat at 180°C in an oil bath at a rate of 50r / min Stir at a constant speed at a rotati...

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Abstract

The invention discloses a method for detecting chlordimeform in aquatic products. The method comprises the following steps: step a, extracting and preparing a to-be-detected sample solution; b, preparing a first sample solution; c, adding a sodium hydrosulfide solution and an ammonium sulfate solution for reaction to prepare a second sample solution; step d, adding calcium hydroxide, chlorine and air to prepare a third sample solution; step e, adding a sodium hydroxide solution to prepare a fourth sample solution; step f, adding a sodium nitrite solution and a hydrochloric acid solution to prepare a fifth sample solution; step g, adding a naphthyl ethylenediamine hydrochloride solution to prepare a sixth sample solution, standing, and observing the color change of the sixth sample solution; and step h, judging a result. The method disclosed by the invention is simple and easy to understand in principle, strong in specificity, high in sensitivity, visual in detection result, high in accuracy of the detection result, free of a large-scale analysis instrument, relatively less in time consumption and extremely low in average analysis cost, is specially suitable for on-site rapid detection of the chlordimeform content in the aquatic product, and has very strong practicability.

Description

technical field [0001] The invention relates to the field of safety detection of aquatic products, in particular to a method for detecting dimeform in aquatic products. Background technique [0002] The pure product of chlordimeform is a white ammonia-like odor crystal, and the industrial product is a light yellow crystal, which is mainly used as a pesticide and insecticide. On October 27, 2017, the International Agency for Research on Cancer of the World Health Organization released a list of carcinogens, and dimeform was included in the list of three types of carcinogens. Dimeform can cause potential harm to the human body through the food chain, and its intermediate metabolites are carcinogenic to the human body. On December 27, 2019, it was listed as a highly toxic drug by the Ministry of Agriculture and the Ministry of Health, and its use is prohibited. [0003] At present, there are mainly two methods for detecting dimeform, one is gas chromatography-mass spectrometry...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/18G01N1/34G01N1/44
CPCG01N21/78G01N33/18G01N1/34G01N1/44
Inventor 熊文明温宝莹李叶青姚鸿州汤胤旻周敏瑜
Owner 广东江门中医药职业学院
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