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Paracoccus with denitrification and phosphorus removal functions and application thereof

A paracoccus and denitrification technology, applied in the field of environmental microorganisms, to achieve the effect of reducing the total phosphorus content

Active Publication Date: 2022-04-08
青岛蔚蓝赛德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] By isolating highly efficient denitrification denitrification and phosphorus removal bacteria, in-depth study of the growth characteristics, denitrification characteristics and application of the bacteria in sewage denitrification and phosphorus removal is of great importance to improve the efficiency and economy of sewage denitrification and phosphorus removal treatment. Theoretical value and practical significance, but among the existing strains that have been screened out so far, there are very few that can simultaneously perform aerobic denitrification and phosphorus removal, and the demand for such strains in practical applications needs to be solved urgently

Method used

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  • Paracoccus with denitrification and phosphorus removal functions and application thereof
  • Paracoccus with denitrification and phosphorus removal functions and application thereof
  • Paracoccus with denitrification and phosphorus removal functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Isolation and Screening of Bacterial Strains

[0027] 1. Enrichment

[0028] Collect the mud-water mixture of the anoxic section of a biological factory sewage treatment station in Gaomi City, Weifang City, Shandong Province, and inoculate it into the enrichment medium (potassium nitrate 2.0g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.2 g, potassium sodium tartrate 20.0g, water 1000mL, pH=7.2±0.1), and cultured in a constant temperature incubator at 30°C for 120 hours to obtain a first-level enrichment solution; the first-level enrichment solution was inoculated at an inoculum size of 10vol%. Into a fresh enrichment medium, culture in a constant temperature incubator at 30°C for 120 hours to obtain a secondary enrichment solution; inoculate the secondary enrichment solution into a fresh enrichment medium at a 10vol% inoculum size, and keep the temperature at 30°C The incubator was left to culture for 120 hours to obtain three leve...

Embodiment 2

[0048] Example 2. Identification of bacterial strain DB133C

[0049] The strain DB133C was sent to the Institute of Microbiology, Chinese Academy of Sciences for strain identification. According to the cell morphology, physiological and biochemical characteristics of the strain, 16SrRNA gene sequence, tauY gene sequence, etc., referring to "Bergey's Handbook of Systematic Bacteriology" and related research papers, DB133C was identified For Paracoccus. The 16S rDNA sequence of the strain is shown in SEQ ID No: 1, the tauY DNA sequence is shown in SEQ ID No: 2, and the cell morphology and physicochemical experiment results of the strain are shown in Table 3.

[0050] Table 3. DB133C cell morphology and physicochemical experiment results

[0051]

[0052]

Embodiment 3

[0053] Example 3. Evaluation experiment of aerobic denitrification ability of Paracoccus strain DB133C

[0054] 3.1 Preparation of bacteria solution

[0055] Activate the Paracoccus strain DB133C, use a sterile inoculation loop to pick up a lawn on the slant of the test tube and inoculate it into 100mL liquid nutrient broth medium (peptone 10g, beef powder 3g, sodium chloride 5g, water 1000mL, pH natural ) at 30°C and 220rpm on a shaker for 24 hours to obtain an activated bacterial liquid, which can be inserted into the evaluation medium.

[0056] 3.2 Effect evaluation experiment of aerobic denitrification capacity

[0057] The activated bacterial solution was inoculated into 100mL denitrification evaluation medium (KNO 3 2.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, potassium sodium tartrate 20.0g, tap water 1000mL, pH = 7.2 ± 0.1), after inoculation, place in 30 ℃, 220rpm shaking table for aerobic culture, detect the total nitrogen content in the medium every 24 hours, th...

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Abstract

The invention relates to a Paracoccus sp. With denitrification and phosphorus removal functions, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.19475. The screened Paracoccus sp. Has anaerobic denitrification and aerobic denitrification effects, and can be used for preparing a phosphorus removal agent. According to the present invention, the strain can efficiently remove the total nitrogen in the water body under the aerobic condition, can reduce the total phosphorus content in the wastewater, and can achieve the total phosphorus removal rate of 63.4% within 72 h when the bacterial liquid inoculation amount is 0.2 vol% and the initial phosphorus content is 13.4 mg / L.

Description

technical field [0001] The invention relates to a paracoccus strain and a microbial bacterial agent containing it, in particular to a paracoccus strain capable of degrading nitrogenous substances and total phosphorus in water and an application thereof, belonging to the technical field of environmental microorganisms. Background technique [0002] With the development of national industrialization and the improvement of people's living standards, the increasing water consumption and the discharge of pollutants have made the problem of water pollution more and more serious. Environmental incidents, public safety incidents and even major social incidents caused by water pollution have seriously affected people's health and social harmony and stability, directly threatening the living space of human beings. [0003] The tail water and aquaculture sewage of urban sewage treatment plants contain different types of nutrients such as nitrogen and phosphorus, and the content is rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 朱文卉刘圣鹏张大飞朱威刘君钟悦高素芹
Owner 青岛蔚蓝赛德生物科技有限公司
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