Composite sensing material based on flexible material and biosensing chip and application thereof

Abstract

The invention discloses a composite sensing material based on a flexible material and a biosensing chip and application thereof, and the composite sensing material is prepared by mixing a microbial solution and a salt solution of transition metal, so that transition metal ions with positive charges are combined with microorganisms with negative charges through electrostatic adsorption; the preparation method comprises the following steps: adding a transition metal organic framework with an organic ligand growing on the surface of a microorganism, drying, and carbonizing under the protection of inert gas, thereby obtaining the composite material, which has a porous structure and a large specific surface area, can well maintain the morphology of the microorganism and expose reaction active sites to the greatest extent. According to the present invention, the ROS released by the cell can be detected with high sensitivity, the biocompatibility is good, the ROS can be directly used for cell culture and growth after the ROS is combined with the conductive ink and printed to prepare the biosensing chip, the in-situ rapid capture and monitoring of the ROS released by the cell are achieved, the important significance is provided for the accurate evaluation of the growth state and the function of the cell, and the application prospect is broad. And huge application potential is shown clinically.

Description

technical field [0001] The invention relates to a biosensing chip, in particular to a composite sensing material based on a flexible material, and to a biosensing chip prepared by using the composite material and its application. Background technique [0002] Reactive oxygen species (ROS) are important signaling molecules in cell activities, mainly including superoxide anion, hydrogen peroxide, singlet oxygen and hydroxyl radicals and other active oxygen-containing compounds in organisms. Abnormal ROS will lead to cell lesions, so real-time monitoring of ROS release from cells is of great significance to the development of life sciences. Among the many ROS detection methods that have been developed, the electrochemical method has the advantages of high sensitivity and fast response, and has become an effective means to detect the release of ROS from cells. At present, the sensing system mainly based on glassy carbon electrodes is used to test the cells growing in the cultur...

AI Technical Summary

Problems solved by technology

This type of sensing system can only be tested on cells grown in a petri dish. Since there is a certain distance between the glassy carbon electrode and the cells in the petri dish, the glassy carbon electrode system cannot quickly capture the ROS released by the cells in situ. In situ real-time monitoring of ROS released from cells cannot be achieved

Therefore, the ROS results measured by such sensors have certain limitations for inferring the true growth state of cells.

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