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Semen protection liquid as well as preparation method and application thereof

A protective liquid and semen technology, applied in the field of animal genetics and breeding, can solve the problems of reducing the activity of enzymes in cells, the decline of sperm fertilization ability, complex animal source components, etc., and achieve no environmental pollution, avoid losses, and low cost Effect

Pending Publication Date: 2022-04-12
BEIJING DABEINONG ANIMAL HEALTH TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A series of damage caused by the solution effect caused by ice crystallization is chemical damage: the redox balance in sperm cells is broken, and the reactive oxygen species (ROS) produced by normal physiological metabolism accumulate in large quantities, which cannot be neutralized by reaction, and are easily combined with the sperm cell membrane. Oxidation of phospholipids can also reduce the activity of intracellular enzymes, reduce motility, and even damage the DNA in the nucleus, resulting in a decline in the fertilization ability of sperm
[0003] Most of the semen protection solutions are aimed at reducing ice crystals and anti-oxidation. In recent years, the existing technology has conducted detailed research on the external conditions that affect the quality of frozen sperm. During the freezing process, the external conditions can be controlled within an appropriate range and the external The impact caused by conditions is minimized. Regarding the research on internal damage, the products currently on the market are mainly based on the addition of penetrating protective agents such as egg yolk, but there are concerns about complex animal-derived ingredients and low safety.

Method used

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  • Semen protection liquid as well as preparation method and application thereof
  • Semen protection liquid as well as preparation method and application thereof
  • Semen protection liquid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The semen storage solution is made of the following components by weight: 16 parts of glucose, 20 parts of citric acid, 35 parts of tris (Tris), 1 × 10 parts of penicillin and streptomycin 6 IU, 1000 parts of double distilled water, the pH value of the prepared semen storage solution is 7.3, and the osmotic pressure is 310mOsm / L;

[0027] The semen protection solution is made of the following components by weight: 16 parts of glucose, 20 parts of citric acid, 35 parts of tris (Tris), 1×10 parts of penicillin and streptomycin 6 IU, 1.0 parts of rhodiola polysaccharide, 0.6 part of astragalus polysaccharide, 1000 parts of double distilled water.

Embodiment 2

[0029] The semen storage solution is made of the following components by weight: 15 parts of glucose, 19 parts of citric acid, 34 parts of tris (Tris), 1 × 10 parts of penicillin and streptomycin 6 IU, 1000 parts of double distilled water, the pH value of the prepared semen storage solution is 7.4, and the osmotic pressure is 300mOsm / L;

[0030] The semen protection solution is made of the following components by weight: 15 parts of glucose, 19 parts of citric acid, 34 parts of tris (Tris), 1×10 parts of penicillin and streptomycin 6 IU, 0.9 parts of rhodiola polysaccharide, 0.5 part of astragalus polysaccharide, 1000 parts of double distilled water.

Embodiment 3

[0032] The semen storage solution is made of the following components in parts by weight: 17 parts of glucose, 21 parts of citric acid, 36 parts of Tris (Tris), 1×10 parts of penicillin and streptomycin 6 IU, 1000 parts of double distilled water, the pH value of the prepared semen storage solution is 7.2, and the osmotic pressure is 320mOsm / L;

[0033] The semen protection solution is made of the following components by weight: 17 parts of glucose, 21 parts of citric acid, 36 parts of Tris (Tris), 1×10 parts of penicillin and streptomycin 6 IU, 1.1 parts of rhodiola polysaccharide, 0.7 part of astragalus polysaccharide, 1000 parts of double distilled water.

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Abstract

The invention discloses a semen protection solution for improving the quality of frozen semen as well as a preparation method and application of the semen protection solution. The semen protection liquid contains active ingredients, and the active ingredients are prepared from rhodiola rosea polysaccharide and astragalus polysaccharide. The semen protection liquid has the effects of resisting oxidation, improving the survival rate of frozen sperms and the like, can be effectively used for semen freezing protection, can effectively improve the plasma membrane integrity rate, the sperm motility, the DNA integrity rate, the acrosome integrity rate and the like of unfrozen sperms, and improves the quality of the frozen sperms. The method has the advantages of wide raw material source, safety, environmental protection, simple preparation process, low production cost and the like.

Description

technical field [0001] The invention belongs to the field of animal genetics and breeding, in particular to a semen protection solution for improving the quality of semen after freezing, a preparation method and application thereof. Background technique [0002] With the development of artificial insemination technology, semen cryopreservation technology is one of the important biotechnological means of mammalian breeding, which can effectively reduce the cost of breeding, make the use of semen not limited by time, place and object of use, and significantly increase the semen of excellent breeding stock The use time and utilization rate, including sampling, dilution, cooling, freezing and a series of complex processes. However, due to the difficulties in the production of frozen semen, the low survival rate and conception rate after freezing and thawing, the semen freezing technology is still not fully mature. During the freezing-thawing process, semen will be damaged to so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 单丽倩刘澜卢贵梅
Owner BEIJING DABEINONG ANIMAL HEALTH TECH
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