Semen protection liquid as well as preparation method and application thereof

Abstract

The invention discloses a semen protection solution for improving the quality of frozen semen as well as a preparation method and application of the semen protection solution. The semen protection liquid contains active ingredients, and the active ingredients are prepared from rhodiola rosea polysaccharide and astragalus polysaccharide. The semen protection liquid has the effects of resisting oxidation, improving the survival rate of frozen sperms and the like, can be effectively used for semen freezing protection, can effectively improve the plasma membrane integrity rate, the sperm motility, the DNA integrity rate, the acrosome integrity rate and the like of unfrozen sperms, and improves the quality of the frozen sperms. The method has the advantages of wide raw material source, safety, environmental protection, simple preparation process, low production cost and the like.

Description

technical field

[0001] The invention belongs to the field of animal genetics and breeding, in particular to a semen protection solution for improving the quality of semen after freezing, a preparation method and application thereof. Background technique

[0002] With the development of artificial insemination technology, semen cryopreservation technology is one of the important biotechnological means of mammalian breeding, which can effectively reduce the cost of breeding, make the use of semen not limited by time, place and object of use, and significantly increase the semen of excellent breeding stock The use time and utilization rate, including sampling, dilution, cooling, freezing and a series of complex processes. However, due to the difficulties in the production of frozen semen, the low survival rate and conception rate after freezing and thawing, the semen freezing technology is still not fully mature. During the freezing-thawing process, semen will be damaged to so...

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