Preparation method of electrostatic spinning membrane for tissue defect repair

Abstract

The invention discloses a preparation method of an electrostatic spinning membrane for tissue defect repair, which comprises the following steps: (i) continuously conveying a solution of a high polymer material through a first liquid supply pipe, and continuously conveying a cross-linking agent solution through a second liquid supply pipe; (ii) mixing the high polymer material solution conveyed from the downstream end of the first liquid supply pipe and the cross-linking agent solution conveyed from the downstream end of the second liquid supply pipe in real time in a main liquid supply pipe positioned at the downstream of the first liquid supply pipe and the second liquid supply pipe to form a spinning solution, and then the spinning solution is conveyed to a spinning electrode of electrostatic spinning equipment through the main liquid supply pipe for spinning, and the viscosity of the spinning solution is controlled according to a preset rule to form an electrostatic spinning film with the fiber diameter corresponding to the preset rule. According to the embodiment of the invention, the viscosity of the spinning solution gets rid of the dependence on the crosslinking reaction time and can be stably controlled.

Description

technical field [0001] The invention relates to the field of medical biomaterials, in particular to a preparation method of an electrospinning film for tissue defect repair. Background technique [0002] Trauma, inflammation, immune diseases, and surgical resection of cancer will all cause soft tissue defects. Soft tissue defects will face multiple problems such as refractory healing, microcirculation disturbance, and susceptibility to infection. In clinical practice, autologous transplantation is often used to deal with larger areas of tissue. However, autologous tissue transplantation is limited, and autologous transplantation will bring secondary trauma to the patient and cause additional pain. Due to the lack of extracellular matrix suitable for cell growth in soft tissue defects, it is difficult for cells to recruit to the damaged part to grow and proliferate to achieve tissue repair, and with the destruction of the microenvironment at the injury site, the tissue often ...

AI Technical Summary

Problems solved by technology

[0005] 1) The gelatin solution that has not been cross-linked or only thermally cross-linked without using a cross-linking agent has a low degree of cross-linking and a fast degradation rate, which cannot meet the needs of the retention time in the body in the repair of tissue defects;

[0006] 2) Using glutaraldehyde as a cross-linking agent, the method of cross-linking the gelatin solution through fumigation often destroys the independent fiber structure, and the adhesion between the fibers makes the material lose its elasticity, making it difficult for cells to adhere and grow in. Moreover, the pollution is large, and the degree of cross-linking is difficult to control stably

In other words, in the spinning process of in-situ cross-linking, it is necessary to match the cross-linking rate of the cross-linking process (making the viscosity of the spinning solution change with time) with the spinning parameters and spinning speed, and small temperature changes, pH Changes in the amino acid content of raw materials, etc. will bring complex changes in the cross-linking process, which will have a great impact on the rate of the cross-linking reaction, which makes it difficult to control the rate of the cross-linking reaction stably, the matching of equipment with the same formula is poor, and the production stability is difficult to control. Not conducive to mass production

Moreover, the adjustable range of the degree of crosslinking is narrow, and it is difficult to adapt to the mechanical performance requirements of different materials for different soft tissue repairs.

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