Near-infrared II region controlled-release nitric oxide nano carrier material as well as preparation method and application thereof

A technology of nitric oxide and nano-carriers, which is applied in the field of biomedical materials, can solve problems such as damage, and achieve high modification flexibility and high-efficiency loading effects

Pending Publication Date: 2022-06-24
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the NO controlled-release nanomaterials based on near-infrared light have achieved certain results in the study of tumor therapy, there are still some shortcomings in the existing NO anti-tumor system: (1) The intelligent NO controlled release of near-infrared light In nanosystems, the commonly used near-infrared light sources are mostly in the near-infrared I region (600nm-900nm), and the near-infrared I region laser will inevitably cause a certain degree of damage to normal tissues
However, most of the existing nano-heterostructures with photothermal effect are in the near-infrared I region, and there are few nano-heterostructure materials for anti-tumor in the near-infrared II region.

Method used

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  • Near-infrared II region controlled-release nitric oxide nano carrier material as well as preparation method and application thereof
  • Near-infrared II region controlled-release nitric oxide nano carrier material as well as preparation method and application thereof
  • Near-infrared II region controlled-release nitric oxide nano carrier material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Preparation of near-infrared II region controlled-release nitric oxide nanocarrier material

[0069] A preparation method of a near-infrared II zone controlled-release nitric oxide nano-carrier material, comprising the following steps:

[0070] (1) heating and preheating the oil bath; Weigh 0.05g cetyl trimethyl ammonium bromide in the flask filled with 10mL water, dissolve ultrasonically, slowly drip 1.29mL NH under the stirring of magnetic stirrer 400rpm 3 ·H 2 O, place the flask in an oil bath at 80 °C, add 0.32 mL of ethyl orthosilicate (TEOS), and stir for 5 h. The mixed solution of absolute ethanol and concentrated hydrochloric acid (volume ratio of absolute ethanol and concentrated hydrochloric acid (mL:mL) =10:1) extraction, centrifugation at 10,000 rpm for 20 min, and washing with absolute ethanol 3 times to obtain a silicon rod;

[0071] (2) Dissolve 2mL silicon rod in 10mL absolute ethanol, add 100μL 0.1mol / L sodium hydroxide solution, sonicate a...

Embodiment 2

[0076] Example 2: Silicon rods prepared in Example 1 - Venus performance test

[0077] The silicon rods, gold nanoparticle-modified silicon rods, silicon rods-Venus (SiNR-AuNSs) and near-infrared II region controlled-release nitric oxide nanocarrier materials (SiNR-AuNSs / BNN6) obtained in Example 1 were observed by transmission electron microscopy.

[0078] The result is as figure 1 shown, where figure 1 a is the TEM image of the silicon rod, figure 1 b is the TEM image of the silicon rod decorated with gold nanoparticles, figure 1 c is the TEM image of silicon rod-Venus, figure 1 d is the TEM image of the controlled-release nitric oxide nanocarrier material (SiNR-AuNSs / BNN6) in the near-infrared region II.

[0079] The particle size distribution of the silicon rods, gold nanoparticle-modified silicon rods, and silicon rod-Venus (SiNR-AuNSs) obtained in Example 1 was observed.

[0080] The result is as figure 2 shown. The particle size test results show that the silico...

Embodiment 3

[0082] Ultraviolet spectroscopy was performed on the silicon rod-Venus (SiNR-AuNSs) obtained in Example 1. The main absorption peak of SiNR-AuNSs is in the range of 1000-1100 nm, which is the near-infrared spectral region, that is, SiNR-AuNSs can be used as a drug carrier material in the near-infrared region II, so a 1064 nm near-infrared laser was selected as the light source.

[0083] Using a 1064nm near-infrared laser as a light source, the photothermal properties of the silicon rod-Venus (SiNR-AuNSs) prepared in Example 1 were measured, and the temperature-time curves were drawn at different powers of 0.5W, 0.75W, and 1.0W lasers for 5 minutes.

[0084] like image 3 and 4 shown. from Figure 4 It can be seen that the silicon rod-Venus under 0.5W laser irradiation was heated from 24.3°C to 60.2°C, and the temperature changed to 35.9°C; the silicon rod-Venus (SiNR-AuNSs) under 0.75W laser irradiation was heated from 24.6°C to 70.1°C , the temperature change is 45.5 °C; ...

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Abstract

The invention discloses a near-infrared II-region controlled-release nitric oxide nano-carrier material as well as a preparation method and application thereof, and belongs to the field of biomedical materials. According to the preparation method, tetraethyl orthosilicate is taken as a silicon source, hexadecyl trimethyl ammonium bromide is taken as a template, after magnetic stirring, the template agent is removed in an extraction mode, and the silicon rod is obtained. The preparation method comprises the following steps: carrying out sulfhydrylation modification on the surface of a silicon rod by using a coupling agent mercaptopropyltrimethoxysilane, connecting the silicon rod with gold nanoparticles through a gold-sulfur bond effect, finally obtaining a silicon rod-gold star by a seed growth method, adding a nitric oxide donor material, and reacting to obtain the near-infrared II region controlled-release nitric oxide nano carrier material. The near-infrared II-region controlled-release nitric oxide nano-carrier material can realize coupling of gas therapy and photo-thermal therapy, and can be used for killing cancer cells.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a near-infrared II zone controlled-release nitric oxide nano-carrier material and a preparation method and application thereof. Background technique [0002] The application of nitric oxide (NO) in gas therapy is affected by its low water solubility, easy diffusion, short half-life, and easy interference by various substances (such as superoxide, hemoglobin, glutathione (GSH), etc.) restrictions. In order to solve these problems, in recent years, researchers have developed a nanocarrier-based NO gas treatment method. This platform uses stimuli-responsive NO nanomedicine to control the release of NO, so that the gas concentration is maintained at the level where it exerts its therapeutic effect, reducing the side effect. From the perspective of stimulus source, stimulus-responsive drugs are mainly divided into endogenous stimulatory drugs and exogenous stimulatory drugs. Exo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/00A61K47/24A61K47/02A61P35/00
CPCA61K41/0052A61K33/00A61K47/24A61K47/02A61P35/00
Inventor 俞思明宋悉玲周小雁赵建夫薛巍
Owner JINAN UNIVERSITY
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