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HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and detection method of Yaohuahuangboo prescription

A technology of Yazuo Niu Ha Zhanbo and fingerprints, applied in the field of chemical detection, can solve the problems of slow development, lack of modern controllable quality control indicators for Yazuo Niu Ha Zhanbo formula, and the quality standard needs to be improved and perfected. Achieve high application value and good reproducibility

Active Publication Date: 2022-06-24
西双版纳傣族自治州民族医药研究所(西双版纳傣族自治州傣医医院)
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slow development of Dai medicine at present, Yalong Niuha Zhanbo prescription lacks modern and controllable quality control indicators, and the quality standard needs to be improved and perfected

Method used

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  • HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and detection method of Yaohuahuangboo prescription
  • HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and detection method of Yaohuahuangboo prescription
  • HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and detection method of Yaohuahuangboo prescription

Examples

Experimental program
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Effect test

preparation example Construction

[0032] 1) Preparation of the test solution: after dissolving the Yajun Niuha Zhanbo granule powder with an organic solvent, ultrasonic extraction, and filtration, the test solution A is obtained;

[0033] 2) Preparation of reference solution:

[0034] Taking the six main active ingredients of Yajun Niuha Zhanbo Formula, namely Danshensu, protocatechuic acid, protocatechuic aldehyde, p-coumaric acid, rosmarinic acid and salvianolic acid B as the reference substance, use 40-70 % methanol is used as solvent to prepare mixed reference solution of different mass concentrations;

[0035] 3) Construction of fingerprint map:

[0036] Draw the test solution A and the mixed reference solution and inject it into a liquid chromatograph for high performance liquid chromatography analysis, that is, get the HPLC fingerprint of Yajunniuhazhanbo formula; establish the concentration of each substance in the mixed reference solution Or the linear relationship between the content and the peak a...

Embodiment 1

[0048] Example 1 Preparation of HPLC fingerprint of Yajunniuhazhanbo granules

[0049] 1. Experimental materials

[0050] Instruments: LC-20A high performance liquid chromatograph (DAD detector, Shimadzu); KQ-300B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); XS105DU analytical balance (Switzerland METTLER TOLEDO).

[0051] Reagents: methanol (batch number: 10044118, Sinopharm Chemical Reagent Co., Ltd.), acetonitrile (batch number: 193502, Thermo Fisher Scientific (China) Co., Ltd.), glacial acetic acid (batch number: 20190530, Tianjin Guangfu Technology Development Co., Ltd.) , pure water (batch number: 3212TJ, Wahaha Pure Water Co., Ltd.).

[0052] Samples to be tested: 11 batches of Yajun Niuha Zhanbo Granules (S1-S11, batch numbers are 200514, 200610, 200612, 200613, 200614, 200710, 200711, 200712, 200713, 200714, 200810, 10g / bag) and dumplings Shield-winged vine Aspidopterys obcordata cane), kidney tea (Lamiaceae plant kidney tea Clerodendranthus sp...

Embodiment 2

[0081] Example 2 Determination of the content of six active ingredients in Yajunniuhazhanbo granules

[0082] 1. Methodological investigation

[0083] 1. Examination of linear relationship

[0084] Prepare mixed reference solution according to item "2.2", inject 2 μL, 4 μL, 8 μL, 10 μL, 12 μL, 16 μL, 20 μL respectively, take the peak area as the ordinate and the injection volume of the reference substance as the horizontal coordinates, perform linear regression, draw standard curves, and fit linear equations, calculate linear range and R 2 value. The results of the linear relationship investigation are shown in Table 1. It can be seen that danshensu, protocatechuic acid, protocatechuic aldehyde, p-coumaric acid, rosmarinic acid, and salvianolic acid B are in the range of 8.0-80 μg·mL, respectively. -1 , 3.2~32 μg·mL -1 , 0.6~6.0 μg·mL -1 , 1.8~18 μg·mL -1 , 7.4~74 μg·mL -1 , 5.6~56 μg·mL -1 There is a good linear relationship with the peak area in the range, R 2 All ...

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Abstract

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and a detection method of a Yaohuahuangboo prescription. According to the present invention, the HPLC fingerprint spectrum and the multi-index content determination are combined for the first time, and the qualitative identification and the content determination are performed on the multi-batch Yaohuahuangboo particles so as to provide the good monitoring and evaluation of the quality of the Yaohuahuangboo particles and the guidance of the standardized production, such that the high application value is provided; a research clue is provided for establishing a quality standard of the Yaohuahuangboo particles with multiple components and multiple indexes, and a solid foundation is laid for deep development of the Yaohuahuangboo particles. The fingerprint spectrum prepared by the method disclosed by the invention not only can be used for qualitatively and quantitatively detecting the Yaohuahuangboo granules at the same time, but also can be used for qualitatively and quantitatively detecting dosage forms such as Yaohuahuahuangboo pills and extractum.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a method for constructing an HPLC fingerprint of Yajun Niu Ha Zhanbo formula and a detection method. Background technique [0002] Yazong Niu Ha Zhanbo is a traditional prescription of Dai medicine. It is contained in the ancient Dai medical classics "Dang Ha Ya Zhao Shu Wan Na". It has a history of more than 1300 years. , Coix root, licorice, has the effect of clearing heat and diuretic fossils, used for the treatment of acute and chronic nephritis, pyelitis, urinary tract infection, kidney, ureter, bladder stones. Xishuangbanna Dai Medical Hospital developed a medical institution preparation "Wulin Fossil Capsule" (Dian Yao Zhi Zi (Z) 20082254K) on the basis of this traditional prescription for clinical application [0003] For 20 years, it has proven efficacy and high safety in the treatment of urinary tract infections and stones. At the same time, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686G01N30/8634Y02A50/30
Inventor 赵应红张村王肖飞玉腊波王云朱晓娟岩罕单王孝蓉周云张兰刘承清陈绿珍刀俊文婉馨王勉
Owner 西双版纳傣族自治州民族医药研究所(西双版纳傣族自治州傣医医院)
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