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Nitrosobacteria agent and preparation method thereof

A technology for nitrosating bacteria agent and nitrous acid bacteria, which is applied in the field of environmental microorganisms, can solve problems such as being unsuitable for waste water, and achieve the effects of reducing adverse effects, being less prone to loss, and facilitating a rapid repair process.

Pending Publication Date: 2022-07-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it is not suitable for treating wastewater containing ammonia nitrogen pollutants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of nitrosative inoculum is as follows:

[0040] (1) Preparation of adsorption carrier: The specific preparation process of cross-linked chitosan is as follows: first, dissolve 2% (W / V) chitosan in 1% (V / V) acetic acid solution for a total of 500 mL, add CaCO 3 Nanoparticles 10g, add 5 times the volume of edible oil, add 10mL Span-80 and stir vigorously. Genipin was added to a final concentration of 20 mM in the aqueous phase, stirring was continued for 24 hours, the precipitate was centrifuged, and washed several times with acetone, hot water, and cold water to remove the oil phase and impurities remaining on the surface of the microspheres. Finally, dehydrate with acetone twice, and air-dry the obtained product at room temperature to obtain a slightly yellow powder, which is a calcium carbonate-embedded chitosan microcarrier. The above-mentioned chitosan carrier was added to the culture system of Candida tropicalis using xylose for adsorption gr...

Embodiment 2

[0044] The preparation method of nitrosifying bacterial agent is the same as Example 1, the difference is that in the preparation process of nitrosifying bacterial agent, according to the mass content of nitrosobacterial microorganisms is 50%, the mass content of adsorption carrier is 50% ratio, The prepared carrier was added to the above-mentioned bacterial cells, mixed evenly, centrifuged and dehydrated to a moisture content of 45%, and vacuum-dried under the conditions of a pressure of 2 kPa and a temperature of 42° C. to prepare a solid bacterial agent B.

Embodiment 3

[0046] The preparation method of nitrosifying bacterial agent is the same as Example 1, the difference is that in the preparation process of nitrosifying bacterial agent, according to the mass content of nitrosobacterial microorganisms, the mass content of 70% and the mass content of adsorption carrier are the proportions of 30%, The prepared carrier was added to the above-mentioned bacterial cells and mixed evenly, centrifugally dehydrated to a moisture content of 35%, and vacuum-dried under the conditions of a pressure of 2 kPa and a temperature of 45° C. to prepare a solid bacterial agent C.

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PUM

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Abstract

The invention discloses a nitrosobacteria agent and a preparation method thereof. The nitrosobacteria agent provided by the invention comprises nitrite bacteria microorganisms and an adsorption carrier, and the adsorption carrier comprises crosslinked chitosan embedded with calcium carbonate and heterotrophic bacteria. The invention also provides a preparation method of the nitrosobacteria agent. The preparation method comprises the following steps: (1) preparing the adsorption carrier; (2) culturing nitrite bacteria type microorganisms; and (3) mixing the microorganisms and the adsorption carrier to prepare the nitrosobacteria agent. The nitrosobacteria agent provided by the invention has the characteristics of high reproduction speed and high biological concentration, and is not easy to lose in the application process.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a nitrosating bacterial agent and a preparation method thereof. Background technique [0002] The main functional microorganisms in the nitrification process are nitrifying bacteria, which are chemotrophic microorganisms. Biological cells can only use the energy stored in the form of ATP. In aerobic metabolism, ATP is mainly synthesized by oxidative phosphorylation of the respiratory chain. The efficiency of ammonia oxidative phosphorylation is very low, and the ATP that can be produced is very limited. These energy are mainly used for electron transitions to higher energy levels, which makes the growth of nitrifying bacteria very slow. Nitrifying bacteria are further divided into ammonia oxidizing bacteria (nitrite bacteria) and nitrite oxidizing bacteria (nitrite bacteria). The nitrification reaction is terminated when it reaches the nitrous a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N1/36C02F3/34C02F3/02C02F101/16
CPCC12N11/14C12N11/10C12N1/36C02F3/34C02F3/341C02F3/347C02F3/02C02F2101/16Y02W10/10
Inventor 高会杰王刚孙丹凤陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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