Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for purification pretreatment of mycotoxins in grains and/or grain and oil and use method of kit

A technology of mycotoxins and kits, which is applied in the field of pretreatment of mycotoxins purification in grains and/or grains and oils, can solve the problems of time-consuming and labor-intensive automatic pre-treatment technology, time-consuming 30 minutes, organic waste liquid pollution, etc. Large-scale production application, avoiding organic waste liquid, and the effect of less manual operation

Pending Publication Date: 2022-07-01
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pretreatment process based on immunomagnetic beads still needs to use 10-20ml of acetonitrile, methanol and other organic solvents to extract the toxin in advance. The extraction process is generally vortexed with an oscillator for 20-30min, and then centrifuged for 5-7min. This process step produces at least 10-20ml organic waste liquid and takes at least 30min
This time-consuming and labor-intensive extraction process not only increases the difficulty of realizing fully automatic pretreatment technology, but also produces organic waste liquid and causes pollution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for purification pretreatment of mycotoxins in grains and/or grain and oil and use method of kit
  • Kit for purification pretreatment of mycotoxins in grains and/or grain and oil and use method of kit
  • Kit for purification pretreatment of mycotoxins in grains and/or grain and oil and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of Mycotoxin Immunomagnetic Beads

[0048] (1) Dissolve recombinant protein G with pH=6.0, 100 mM MES buffer to obtain a recombinant protein G solution with a concentration of 10 mg / mL.

[0049] (2) Take 4 mL of NHS magnetic beads (20% (v:v), 10-30 μm), and wash the magnetic beads twice with absolute ethanol. According to the coupling ratio of 6 mg of recombinant protein G to 1 mL of NHS magnetic beads, the recombinant protein G solution was added to the NHS magnetic beads, and then a certain amount of MES buffer was supplemented, so that the whole system was carried out in the MES buffer, at room temperature, slowly Mix and incubate for 2 hours.

[0050] (3) After the incubation, the magnetic beads were washed with 0.1% PBST and PBS solution twice, and the volume was adjusted with PBS solution to prepare recombinant protein G immunomagnetic beads.

[0051] (4) Dilute the fumonisin antibody with MES buffer to prepare a fumonisin antibody dilution. Accordi...

Embodiment 2-6

[0055] The same as Example 1, the difference is only that the fumonisin antibody in step (4) is replaced by aflatoxin antibody, zearalenone antibody, vomitoxin antibody, and fumonisin antibody, respectively, to obtain aflatoxin immunity. Magnetic beads, zearalenone immunomagnetic beads, vomitoxin immunomagnetic beads, fumonisin immunomagnetic beads.

Embodiment 7

[0057] 1) Provide a kit for aflatoxin purification pretreatment and a method of using the same

[0058] The kit includes sample well 1 , reaction well 2 , cleaning wells (first cleaning wells 3 and 4 and second cleaning wells 5 ), elution wells 6 and magnetic bead storage wells 7 .

[0059] Take 50 μL of the uniformly mixed aflatoxin immunomagnetic bead solution prepared in Example 2, 0.25 mL of peanut oil quality control sample, and 0.45 mL of 0.5% PBST buffer (containing 0.5% Tween-20) and place them in reaction well 2 of the kit; Take 1 mL of 0.5% PBST buffer (containing 0.5% Tween-20) in the first washing well 3 and the first washing well 4, take 1 mL of PBS solution in the second washing well 5; take 0.5 mL of methanol in the elution well 6 middle. Take 0.5 mL of deionized water into the magnetic bead storage well 7.

[0060] Place the kit in an automatic mycotoxin purifier. Install the magnetic rod cover on the automatic mycotoxin purifier. A fully automatic purifica...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a kit for purification pretreatment of fungaltoxin in grain and / or grain and oil and a use method of the kit, the kit comprises a deep-well plate reagent strip, the deep-well plate reagent strip comprises a plurality of fungaltoxin immunomagnetic bead reagent strips arranged side by side, the mycotoxin immunomagnetic bead reagent strip comprises a reaction hole, a cleaning hole, an elution hole and a magnetic bead preservation hole which are arranged in sequence; and the reaction holes contain reaction liquid and mycotoxin immunomagnetic beads. According to the method, the mycotoxin in the grain oil or grain powder can be rapidly purified and enriched by utilizing the action of the reaction liquid and by virtue of auxiliary conditions such as oscillation / ultrasound of a full-automatic purification instrument, and compared with a traditional treatment process, the steps such as extraction, centrifugation and filtration are not needed, so that the pretreatment time is shortened, the pretreatment process is simplified, and the cost is reduced. And moreover, organic waste liquid is avoided, so that compared with the traditional process, the method disclosed by the invention is more environment-friendly and has higher economic value and social value.

Description

technical field [0001] The invention relates to the technical field of mycotoxins purification pretreatment. More specifically, it relates to a kit for pre-purification of mycotoxins in grains and / or grains and oils and a method of using the same. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi during the growth and reproduction process. They are widely present in crops and their products, and have great harm to human health. Grain and oil are prone to produce harmful mycotoxins due to fungal contamination caused by factors such as climate, environment and transportation conditions during the growth, harvesting, and oil refining and storage of oil crops. At present, the detection methods of mycotoxins in grains and grains mainly include thin layer chromatography, enzyme-linked immunosorbent assay, colloidal gold immunochromatography, high performance liquid chromatography, high performance liquid chromatography tandem mass spectrometry a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/543
CPCG01N1/28G01N33/54326
Inventor 王松雪倪保霞叶金陈金男
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com