Yarrowia lipolytica, application thereof and method for producing erythritol through fermentation
A technology of Yarrowia lipolytica and erythritol, which is applied in the field of fermentation to achieve the effects of less fermentation by-products, favorable crystallization and separation, and high yield
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Embodiment 1
[0049] This example is used to illustrate the isolation, mutagenesis and fermentation effect verification of Yarrowia lipolytica CGMCC No. 22632.
[0050] (1) Strain isolation
[0051] From the naturally fermented wine grape mash in the Shacheng production area of Huaizhuo Basin, my country, samples were taken at different stages of fermentation, and the samples were diluted with gradients and then spread on a WL solid medium plate. The strains with better growth were selected, inoculated on the screening medium, cultured at 30°C for 3-4 days, and single colonies with better growth and red color were selected, inoculated into the activation medium, cultivated at 30°C and 200rpm for 24h, press 10% (v / v) inoculum was transferred to the fermentation medium (the composition ratio of the fermentation medium was: 220g / L glucose, 5g / L yeast extract, 1g / L potassium dihydrogen phosphate, magnesium sulfate 0.2g / L, ammonium sulfate 5g / L, pH 6), cultured at 32°C at 250rpm for 120h, and...
Embodiment 2
[0061] The composition ratio of the fermentation medium is: glucose 325g / L, yeast extract powder 5g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, ammonium citrate 5g / L, pH 5.8.
[0062] The preserved CoY1-M1 glycerol bacteria were inoculated into 300 mL of activated medium at 3‰ (v / v), and cultured at 30 °C and 200 rpm for 20 h (OD). 600 =10), inoculated into a 5L fermentor containing fermentation medium at a 10% (v / v) inoculum. After the inoculation, the liquid filling amount of the fermenter is 60% by volume, the fermentation temperature is controlled at 32°C, the rotating speed is 200-1000rpm, the ventilation volume is 0.5-1vvm (L / min·L), and the dissolved oxygen is controlled at 25-32vol %. In the early stage of fermentation, samples were taken every 12 hours to detect the glucose concentration, the fermentation rate was estimated according to the glucose concentration, and the fermentation end point (exhausted glucose) was determined according to the gl...
Embodiment 3
[0066] The composition ratio of the fermentation medium is: glucose 220g / L, yeast extract powder 1g / L, corn steep liquor powder 5g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.2g / L, ammonium sulfate 5g / L, spit Temperature 80 0.25g / L, pH6.
[0067] The preserved CoY1-M1 glycerol bacteria were inoculated into 100 mL of activated medium at 3‰ (v / v), cultured at 30°C and 200 rpm overnight (16 h), and then transferred to 600 mL of fermentation medium at 10% (v / v) inoculum. , 30 ℃ 240rpm cultured for 16h to obtain the seed liquid (OD 600 = 25).
[0068] The seed liquid is all connected to the 10L fermenter, the liquid filling amount of the fermenter after the inoculation is 60% by volume, the fermentation temperature is controlled at 32 ° C, the rotating speed is 200-900rpm, and the ventilation rate is 0.5-1vvm (L / min·L), The dissolved oxygen content is controlled at 20-25vol%. In the early stage of fermentation, samples were taken every 12 hours to detect the gluc...
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