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Novel polypeptide and application thereof in preparation of medicine for treating skin wound or mucosal lesion

A technology for skin wounds and new peptides, applied in skin care preparations, drug combinations, skin diseases, etc., can solve problems such as difficult treatment, lingering disease, slow onset, etc., and achieve the effect of promoting repair, small molecular weight, and high purity

Pending Publication Date: 2022-07-22
SICHUAN GOODDOCTOR PANXI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chronic gastritis often occurs in middle-aged and elderly people. The onset is related to age but has nothing to do with gender. The onset is slow and lingering, and the treatment is difficult.

Method used

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  • Novel polypeptide and application thereof in preparation of medicine for treating skin wound or mucosal lesion
  • Novel polypeptide and application thereof in preparation of medicine for treating skin wound or mucosal lesion
  • Novel polypeptide and application thereof in preparation of medicine for treating skin wound or mucosal lesion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening of new polypeptide molecules

[0040] (1) The new peptide is from Sichuan Good Doctor Panxi Pharmaceutical Co., Ltd.

[0041] (2) EGFR protein target affinity screening

[0042] EGFR affinity screening: After dissolving the freeze-dried polypeptides of the present invention into powders with protein buffers, a polypeptide compound sample solution was prepared, and an appropriate concentration of EGFR solution was incubated with the polypeptide compound sample solution at room temperature for 50 minutes, and then affinity screening was performed. The centrifugation speed was 90000rpm and the time was 70min. After centrifugation, the samples were taken out and divided into upper, middle and lower layers. Appropriate volumes of acetonitrile and water were added to each layer to precipitate proteins, and the supernatant was centrifuged to collect the supernatant for mass spectrometry analysis.

[0043] EGF polypeptide was used as a positive compound con...

Embodiment 2

[0048] Embodiment 2 The chemical synthesis method of new polypeptide

[0049] Using the conventional solid-phase synthesis method of an automatic peptide synthesizer, after resin swelling, deprotection, washing, amino acid dissolution, amino acid activation, and condensation, the chemical synthesis of new peptides was carried out, and 10 peptides were obtained. After mass spectrometry analysis, the structure was confirmed. Figures 1 to 10 is the mass spectrum of the new peptide.

Embodiment 3

[0050] Example 3 The effect of the new polypeptide PA2 on the proliferation of Hacat cells

[0051] Adjust the concentration of human immortalized keratinocytes (HaCaT cells) to 1.0 × 10 5 ~5.0×10 5 / mL for subculture at 37°C, 5% CO 2 Incubate for 24-36 hours under conditions for biological activity detection. Digest the cells with 0.25% trypsin for 5 minutes, add 1640 whole blood medium with more than 1 times the volume of trypsin to terminate the digestion, collect the cell suspension, centrifuge at 1000 RPM for 3 minutes, discard the supernatant, and add 2 mL of 1640 whole blood medium to resuspend the cells , take 20uL of cell suspension, stain with AOPI, and then use a cell counter to detect the concentration of cells in the suspension, and use 10% serum concentration of 1640 medium to make a concentration of 5 × 10 4 Inoculated in 96-well cell culture plate, 100 μL per well, i.e. 5000 cells / well, at 37°C, 5% CO 2 Incubate overnight under conditions. After 24 hours, ...

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PUM

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Abstract

The invention belongs to the field of medicines and daily chemicals, and particularly relates to a group of novel polypeptides and application thereof in preparation of medicines for treating / or preventing skin wounds or mucous membrane injury. The novel polypeptide provided by the invention can be combined with an epidermal growth factor receptor EGFR to play a biological effect, and can be used for preparing medicines or daily chemical products for treating / or preventing skin or mucous membrane diseases, acute and chronic gastrointestinal diseases, tumors and other related diseases.

Description

technical field [0001] The invention belongs to the field of medicines and daily chemicals, and particularly relates to a group of novel polypeptides and their application in the preparation of medicines for treating and / or preventing skin wounds or mucosal damage. Background technique [0002] Epidermal growth factor receptor (EGFR) is a multifunctional glycoprotein widely distributed on the cell membrane of various tissues of the human body, and is an oncogene of avian erythroblastic leukemia virus (v-erb-b). Homologue is one of the four members of the HER / ErbB family, so it is also known as HER1 or ErbB-1. EGFR and its ligands are part of the cell signaling system. Studies have shown that EGFR gene copy number increase or overexpression can promote the transformation of normal cells and the metastasis of malignant tumors. The signaling network of EGFR plays an important role in the formation and development of tumors. Since the EGFR gene was first cloned in 1984, studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K5/10A61K38/07A61K38/08A61K8/64A61P17/02A61P35/00A61P1/04A61P1/02A61Q19/00
CPCC07K7/06C07K5/10A61K38/07A61K38/08A61K8/64A61P17/02A61P35/00A61P1/04A61P1/02A61Q19/00A61K2800/10
Inventor 耿福能
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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