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Preparation method and application of guava leaf extract

A guava leaf and extract technology, which is applied in the field of preparation of guava leaf extract, can solve the problems of unreported anti-inflammatory activity, high quality requirements of medicinal materials, and many impurities in effective parts, and achieve broad market prospects and development value , solution penetration rate is fast, the effect of low cost

Pending Publication Date: 2022-07-29
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese patent CN 201110100466.5 introduces "Guava leaf extract with hypoglycemic effect and its preparation method and application". The preparation method of the acid part mainly uses the purification process of alcohol extraction and water precipitation. This process has high requirements on the quality of medicinal materials, and the obtained effective parts have more impurities and some active ingredients are lost.
However, using silica gel column chromatography to prepare a large number of samples is time-consuming, labor-intensive, costly and long-term
And the anti-inflammatory activity of the total triterpenoids in guava leaves has not been reported

Method used

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  • Preparation method and application of guava leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] After 3 kg of fresh guava leaves were dried in the sun, they were extracted twice with 10 times the amount of 70% ethanol, respectively, for 1 hour each time, and the 2 extracts were combined, filtered, and concentrated under reduced pressure to alcohol-free to obtain 3 L of concentrated solution; Extracted with petroleum ether for 1 time, then extracted with 0.5 times the amount of ethyl acetate for 3 times, combined the ethyl acetate fractions, and concentrated under reduced pressure to obtain 50 g of ethyl acetate fraction extract.

[0030] Take the ethyl acetate part extract prepared by the above method, mix it with silica gel neutral alumina according to the mass ratio of 1:3, vacuum dry and grind at 50 °C, and dry the sample for neutral alumina column chromatography. , eluted with 100% dichloromethane, dichloromethane:methanol 10:1, dichloromethane:methanol 10:2, dichloromethane:methanol 2:1, dichloromethane:methanol 1:1 , the amount of eluent for each gradient co...

Embodiment 2

[0047] 1) Cell lines and reagents: RAW264.7 (mouse macrophages). Fetal bovine serum, DMEM medium, and RPMI-1640 medium were purchased from Shanghai Haoran Biotechnology Co., Ltd. EDTA trypsin and PBS buffer were purchased from Shanghai Biyuntian Reagent Company, China. MTT reagent was purchased from Shanghai Enyi Biotechnology Co., Ltd. LPS (lipopolysaccharide) was purchased from Beijing Soleibo Technology Co., Ltd.

[0048] 2) Test drugs: the guava leaf extract prepared in Example 1 of the present invention; the guava leaf extract prepared in Comparative Examples 1-5. The positive control drug was hydrocortisone.

[0049] 3) Cell culture and drug effects: mouse macrophages RAW264.7 are adherent cells. After the cells are revived, use DMEM medium (containing 10% fetal bovine serum) in an incubator containing 5% carbon dioxide at 37°C. Culture the cells, take the cells in the logarithmic growth phase, digest with trypsin and adjust the suspension density to 5×10 5 cells / mL...

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Abstract

The invention belongs to the field of natural medicines, and particularly relates to a preparation method and application of a guava leaf extract. The preparation method of the psidium guajava leaf extract comprises the following steps: (a) taking fresh psidium guajava leaves as a raw material, carrying out ethanol reflux extraction, and then extracting with petroleum ether and ethyl acetate; and (b) loading the ethyl acetate part extract obtained in the step (a) on a neutral alumina column, carrying out gradient elution by using a dichloromethane-methanol system, detecting by using a thin-layer chromatography, collecting and combining similar parts, and carrying out vacuum concentration to obtain a sample. The guava leaf triterpenoid extract is obtained from guava leaves by adopting a neutral alumina chromatographic column method. Pharmacological experiments prove that the guava leaf extract prepared by the preparation method disclosed by the invention has excellent anti-inflammatory activity.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to a preparation method and application of a guava leaf extract. Background technique [0002] Inflammation is a defense response of living tissue with a vascular system to stimulation. Inflammation occurs only in vertebrates and humans, and is characterized by redness, swelling, pain, etc., as local and systemic defense responses centered on the vascular system. Vascular reaction leads to local vasodilation and slow blood flow, and blood components such as plasma and neutrophils in the blood vessels leak into the tissue: confine and kill the damage factor, clear and absorb the necrotic tissue. Vascular responses are a major feature of the inflammatory process and a central link in defense. [0003] Usually, inflammation is beneficial and is the body's own defense mechanism, but sometimes inflammation is also harmful. At present, malignant tumors are still the main di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P29/00A61K127/00
CPCA61K36/61A61P29/00A61K2236/333A61K2236/35A61K2236/39A61K2236/55A61K2236/51
Inventor 孙敬勇李敏茹牟晓东刘蕾赵晓东
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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