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Surface plasmon resonance detection with high angular resolution and fast response time

A surface plasmon and sensor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of low response time, reduced response time, reliability depends on the accuracy of data points, etc.

Inactive Publication Date: 2005-08-24
佛罗里达国际大学董事会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] As mentioned above, these methods suffer from two main drawbacks: low response time and limited angular resolution
The second method has no mechanical movement, but simultaneously sensing multiple channels (for example, 1024 in a typical linear diode array) reduces response time
To achieve higher angular resolution, both methods require a large distance between the sample and the detector, which makes the setup more susceptible to mechanical noise and thermal drift
But large distances degrade the quality of the detected beam, making SPR bulky
For a given distance between the sample and the detector, the solution of the first method is limited by the accuracy of measuring the angular position of the prism
The solution of the second method is limited by the number of channels (pixels) of the photodetector array and the noise level of the intensity measured in each channel
An improved solution can be obtained by using a software program to fit the data obtained by the first or second method, but this fitting process requires additional time and its reliability depends on the accuracy of each data point measured sex
Another problem with the second approach is that the beam intensity spreads across multiple channels, reducing the signal-to-noise ratio and thus limiting the solution

Method used

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  • Surface plasmon resonance detection with high angular resolution and fast response time
  • Surface plasmon resonance detection with high angular resolution and fast response time
  • Surface plasmon resonance detection with high angular resolution and fast response time

Examples

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Effect test

example 1

[0046] SPR device 1

[0047] In an SPR setup, a BK7 plano-cylindrical lens (Melles Griot) was used as the prism. A prism is closed, but not a perfect half cylinder. On the prisms, a 50-nm-thick gold film deposited on a BK7 glass slide in ultra-high vacuum has a coefficient matching that of the liquid. To reduce surface contamination, the gold films were heat-treated in a hydrogen flame before each experiment. A 5mW diode laser (l=635nm, Hitachi brand) is driven by a domestic laser controller. After calibration, a 14mm partial length (partial length) lens is used to focus on the gold film through a prism. Light reflected by the gold film was detected using a two-element photodiode detector (Hamamatsu Corporation, Model S2721-02) mounted on a precision translation stage. The photocurrents from the two cells (A and B) were converted into voltages using domestic circuits. The circuit also calculates the difference signal A-B and the sum signal A+B, and then sends these two sig...

example 2

[0050] SPR device 2

[0051] Another SPR setup used a BK7 plano-cylindrical lens (Melles Griot) as the prism. The BK7 glass sheet on the prism is coated with a 45nm thick silver or gold film using a vacuum coating machine, and its coefficient matches that of the liquid. White light from a 150W xenon lamp (oriel window) is sent to a monochromator. The monochromatic light with a bandwidth of 0.5nm emitted by the monochromator is calibrated and focused onto the silver film by a 14mm partial length lens through a prism. Light reflected by the silver film was detected using a two-element photodiode detector (Hamamatsu Corporation, Model S2721-02) mounted on a precision translation stage. The prism was rotated in each measurement such that a black line corresponding to the SPR inclination was positioned at the center of the laser beam. The reflected light falling on the two cells of the photodetector is then balanced by adjusting the position of the photodetector to A-B equal to ...

example 3

[0054] Effect of electron density on SPR

[0055] Figure 6 Shown is the SPR tilt position of a gold film (electrode) covered with a single layer of organic matter (thiopropionic acid or MPA) in 50mM phosphate buffer as the electrode voltage varies linearly from -0.2V to 0.3V (control Ag / AgCl). . The tilt position shifts by about 0.0008 degrees per 100mV, which is too small to be easily detected using conventional SPR devices. It is known that the electrode voltage can change the SPR dip position by changing the electron density in the metal film. The shift measured here is much smaller than for a bare gold electrode because the presence of MPA reduces the surface capacitance, and thus the electron density changes for a given change in voltage.

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Abstract

A device and method of detecting surface plasmon resonance for sensing molecules or conformational changes in molecules with high resolution and fast response time is disclosed. Light from a light source (14) is focused through a prism onto a metal thin film (15) on which sample molecules to be detected are adsorbed. The total internal reflection of the laser / incident light is collected with a differential position or intensity sensitive photo-detecting device instead of a single cell or an array of photo-detectors (12) that are widely used in previous works. The ratio of the differential signal to the sum signal of the differential position or intensity sensitive photo-detecting device (12) provides an accurate measurement of the shift in the surface plasmon resonance angle caused by the adsorption of molecules onto the metal films (15) or by conformational changes in the adsorbed molecules. The present invention requires no numerical fitting to determine the resonant angle and the setup is compact and immune to background light, The methods and sensors of this invention can be used in numerous biological, biochemical, and chemical applications such as measuring subtle conformational changes in molecules and electron transfer reactions can be studied.

Description

[0001] Related application reference [0002] This application claims the benefit of US Provisional Patent Application Serial No. 60 / 134,482 filed May 17, 1999. [0003] This invention was made with government support under CHE-9818073 awarded by NSF and GM-08205 awarded by NIH. technical field [0004] The present invention relates generally to methods and measuring instruments used in biological, biochemical and chemical detection, and in particular to the use of surface plasmon resonance (SPR) with ultra-high resolution and ultra-fast response time to detect molecules or monitor molecular Methods, Apparatus and Use of Instruments for Structural and Electron Changes. Background technique [0005] Surface plasmon resonance (SPR) is the plasmonic oscillation of free electrons that exists at the edges of metals. These oscillations are affected by the refractive index of the material adjacent to the metal surface. This phenomenon is used to detect small changes in the refra...

Claims

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Application Information

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IPC IPC(8): G01N21/27G01N21/55G01N27/416
CPCG01N21/553
Inventor 陶农建S·布萨尔德黄文略
Owner 佛罗里达国际大学董事会
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