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Rose extract and its application

A technology of rose extract and extraction method, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems that have not been reported, and achieve the effect of rich plant resources, easy extraction, and anti-aging

Inactive Publication Date: 2005-11-02
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, extracting antioxidant active substances from roses has not been reported so far

Method used

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  • Rose extract and its application
  • Rose extract and its application
  • Rose extract and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Rose flower extract (F 21 ) preparation and purification

[0020] Collect the plant roses produced in Fujian, chop the dried plant flowers, soak them in water, and boil them under reflux for 2 hours. , manufactured by Beijing Sihuan Scientific Instrument Factory).

[0021] Take 50mg of dry powder and dissolve it in an appropriate amount of ammonium acetate buffer (10mM, pH4.5), load it on a CM-32 cation exchange cellulose chromatography column, and then use 10mM pH4.5, 200mM pH4.5, 400mM pH8. 0 ammonium acetate buffer solution to elute sequentially, automatically collect step by step, and measure the antioxidant activity of each tube.

[0022] The separated active substance was applied to a Sephadex G-75 chromatographic column, collected by elution with phosphate buffer (50 mM, pH 7.0), and freeze-dried.

[0023] The composition of the extract is determined

[0024] f 21 Three kinds of active ingredients F were separated through the above-mentioned CM-32 cation exc...

Embodiment 2

[0057] Mix well, pour into 1cm optical diameter cuvette after 10 minutes, adjust to zero with distilled water, and compare color at wavelength of 550nm.

[0058] Definition: One nitrite unit (NU / mg.prot) is the corresponding SOD amount when the SOD inhibition rate reaches 50% per milligram of tissue protein in 1 ml of reaction solution. After calculation, the average value of SOD enzyme activity of 6 mice in the administration group was 739.69NU / mg.prot. The average value of SOD enzyme activity of 6 rats in the control group was 624.89NU / mg.prot, and the average increase was 114.80NU / mg.prot after administration.

Embodiment 3

[0060] The selection, grouping, administration and administration time of the mice were the same as in Example 2. After 30 days of administration, the experimental mice were killed by neck dislocation, and the liver tissue was taken to make 10% liver homogenate. After centrifugation, 1ml of the supernatant was taken, and 10μl of absolute ethanol was added. 100 110μl, ice bath for 5 minutes. Dilute the sample 200 times and add to the reaction system, add 2ml of enzyme solution to the assay tube, mix with 1ml of 10mM PBS, measure the change of absorbance at 240nm for 30 seconds, and calculate the CAT enzyme activity.

[0061] The average CAT enzyme activity of the 6 mice in the treatment group was 491.01K / g.Hb, and the average CAT enzyme activity of the 6 mice in the control group was 361.20K / g.Hb. The average activity of the CAT enzyme in the treatment group was 129.81K higher than that of the control group / g.Hb.

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PUM

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Abstract

An extract of Rosa rugosa Thunb is disclosed, and contains rose extract (30%) which contains polyose (84.74%), protein (8.57%) and gallic acid derivative (6.69%). It plays such roles as suppressing erythrocyte haematolysis by 100% and peroxidization of tissue lipid by more than 90% and increasing the activity of antioxidant enzyme. It can be used to prepare antioxidant for preventing and treating diseases and increasing immunity.

Description

Technical field [0001] The invention relates to the extraction of natural plants, especially the rose extract. The extract has natural antioxidant activity and can be made into an antioxidant for preventing and treating diseases, improving immunity of the body, and as a health care product for prolonging life. Background technique [0002] Judging from the current research results, oxygen free radicals are not only related to aging, but also to most of the common major human diseases, from cardiovascular and cerebrovascular diseases with the highest human mortality rate to the most terrifying cancers and AIDS in humans, all of which are consistent with Free radicals are closely related. By preventing the generation of free radicals and removing existing free radicals, the purpose of disease prevention and treatment is achieved. At present, the artificially synthesized antioxidants have certain toxicity and mutagenicity, and their reuse is greatly restricted. Therefore, rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P39/06
Inventor 刘方牛淑敏吴子斌
Owner NANKAI UNIV
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