Preparation method for biologic photosensitive protein-nanometer semiconductor composite photoelectric electrode
A nano-semiconductor and bio-light technology, which is applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, can solve the problems of weakening mediator oxidation and affecting the photoelectric conversion efficiency of photoelectrodes, and achieves the promotion of charge separation and the preparation method. Complex, Life-Prolonging Effects
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Embodiment 1
[0014] Example 1: Using spinach as a raw material, crude chlorophyll was obtained, and DEAE-Sephaerose CL-6B column chromatography was performed at 4°C. First wash with about 100ml of petroleum ether, then use petroleum ether containing 0.5% n-propanol to elute the plant pigments successively, discard the carotenoids that elute first, collect the chlorophyll a that elutes next, and put them in the N 2 After drying in medium, dissolve in 1 g / L pyridine solution and store in dark state at 4°C. Take 10ml diluted plant chlorophyll pyridine solution (0.1mmol / L), in N 2 Dry in medium, add 0.5ml glacial acetic acid. The resulting plant pheophytin in N 2 After drying in medium, dissolve in 10ml acetone and store in dark state at 4°C. A certain volume of RC solution is taken, and 10% volume of the acetone solution of the above-mentioned plant pheophytin is added, so that the molar ratio of plant pheophytin to RC is greater than 20%. After stirring, keep warm at 43.5±0.5°C for a per...
Embodiment 2
[0015] Embodiment two: adopt porphyrin n-propyl ether alcohol as replacement pigment, add the acetone solution of 10% volume replacement pigment in certain volume of RC solution, make the molar ratio of porphyrin n-propyl ether alcohol and RC greater than 25%. After stirring, keep warm at 43.5±0.2°C for a period of time, then wash off free chlorophyll with about 600ml of 0.09mol / L NaCl dilute TL buffer solution on the DEAE-cellulose chromatography column (DE-52), and use TL buffer solution of 0.15mol / L NaCl elutes the modified RC, then spreads the RC solution to be purified on a 10%-40% sucrose gradient, centrifuges at 4°C for 16h, and takes the first RC from top to bottom. The second layer of pale yellow supernatant is the purified pigment replacement RC. Dilute the pigment-substituted RC with pH 8.0 Tris-HCl buffer solution to obtain a diluted solution of mutated RC with a concentration of 2 mM, and store it in a refrigerator at 4°C. mesoporous WO 3 -TiO 2 The preparation...
Embodiment 3
[0016] Embodiment 3: Using porphyrin dimethyl ether diol as a replacement pigment. The preparation of the Tris-HCl buffer solution of porphyrin dimethyl ether diol replacing RC is the same as in Example 2. mesoporous WO 3 -TiO 2 The preparation of the thin film electrode is the same as that in Example 1. Pigment Replacement RC—Mesoporous WO 3 -TiO 2 The preparation of the composite photoelectrode and the measurement process of photoelectric properties are the same as in Example 2. The pigment replaces RC-mesoporous WO 3 -TiO 2 The short-circuit photocurrent of the composite photoelectrode under the above conditions is about 2.6 microamperes, which is the same as that of mesoporous WO prepared and tested under the same conditions without RC. 3 -TiO 2 3.7 times the electrode short-circuit photocurrent (0.7 microampere). The composite membrane electrode prepared above has good reproducibility. The composite membrane electrode was stored in a refrigerator at 4°C, and sho...
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