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Preparation method of broad-spectrum bioagricultural chemical for preventing disease

A biopesticide, insecticide and disease prevention technology, applied in botany equipment and methods, biocides, animal repellants, etc., can solve the problems of separation, screening, and preparation methods that have not been reported, and achieve high yield, The effect of low residual amount and short production time

Inactive Publication Date: 2006-01-25
成都川农邑邦农业发展有限公司
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AI Technical Summary

Problems solved by technology

So far, the method for separating, screening, and preparing the biopesticide of this Streptomyces genus from soil microorganisms has not been reported.

Method used

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  • Preparation method of broad-spectrum bioagricultural chemical for preventing disease
  • Preparation method of broad-spectrum bioagricultural chemical for preventing disease
  • Preparation method of broad-spectrum bioagricultural chemical for preventing disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Determination of Sichuan Variety of Streptomyces freundii

[0044] Using Gaoshi No. 1 medium for the growth of Streptomyces, a large number of Streptomyces were screened from the soil, and the pests were widely fed with the fermented products in the laboratory and the resistance to fungal diseases was detected on the PDA plate at 28°C. Streptomyces strain numbered S20-6, which is broad-spectrum, insecticidal and disease-resistant, and performs well.

[0045] After culturing the strains on Gao's No. 1 medium and glucose-asparagine agar at 28°C for 7 days, observe the hyphae and surface characteristics according to conventional methods, and culture them on Gao's No. 1 medium, glucose-asparagine agar, Inorganic salt starch agar, oat flour agar, yeast malt extract agar, and potato extract agar were cultured at 28°C for 7 days, and the characteristics of mycelium were observed. According to the Hasegawa T fast thin-layer chromatography method, the amino acid and sugar layer...

Embodiment 2

[0047] Preparation of biopesticides

[0048]A. Production of primary seed bacteria: For large-scale expanded cultivation, the bacterial strains are subjected to seed-level enlarged cultivation. The seed medium is made of 2.5-3.5% corn flour, 0.4-1% wheat bran powder, 10-15% potato, 2.5-2.8% nutrient salt, and 40%-50% water, which is used as the carbon required for the growth of the strain source, nitrogen source, energy and growth factors, the total amount of the medium is 8500-9000 kg, steam sterilization at 120°C for 30 minutes, inoculate the above-mentioned Streptomyces griseignis Sichuan variety into the medium, and add poly Ether and soybean oil are defoamed. Through fermentation operation, the seed strain is obtained.

[0049] B. Fermentation: The seed strain is used as the source of the strain, and the expanded culture is carried out to obtain the culture product. Application: Corn flour, wheat bran flour, potato liquid, inorganic nutrient salts and auxiliary materia...

Embodiment 3

[0053] Laboratory Antagonism and Pesticide Tests

[0054] The potato extract was used for laboratory fermentation to obtain fermentation products. Through laboratory antagonism and insecticidal tests, it is shown that the fermentation product has a strong antagonistic effect on crop fungal pathogens such as rice sheath blight, rice blast, rice smut, Aspergillus flavus, rape sclerotinia, and vegetable fusarium wilt. It also has a killing effect on rice bract worms and vegetable cabbage worms. Fermentation products are obtained by fermenting the PDA medium. Through the control effect experiment in the field, the highest control effect on fungi can reach 91%, and the average control effect can reach more than 80%. The field control effect on rice bract moth reaches 92%, and the control effect on cabbage caterpillar reaches 89%. Cytological studies have shown that effective fermentation products have perforation, teratogenic, constriction, and fracture effects on pathogenic myc...

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Abstract

A process for preparing the broad-spectrum biological agricultural chemical to prevent diseases and insectcide includes such steps as culturing grisostreptomycete freundii S20-6, seed-class amplifying culture, strain-class amplifying culture, fermenting, concentrating, and mixing with additive. Its advantages are short period, high output rate, and high effect to more disease, including rice Hypochnus, rotten neck, vegetable blight, etc.

Description

Technical field: [0001] The invention relates to a preparation method of a biological pesticide, in particular to a preparation method of a biological pesticide capable of killing insects and preventing diseases obtained by inoculating a Streptomyces strain in an improved Streptomyces medium for fermentation. Background technique: [0002] The use of microbial metabolites to prepare agricultural antibiotics has been widely used in the control of crop pests. In 1973, the Shanghai Pesticide Research Institute isolated St. hygroscopicus var. jinggangensis Yan from the soil in the Jinggangshan area of ​​Jiangxi. Up to 80% or more. In 1963, the endotherapin purified from Streptomyces hygroscopicus by the Chinese Academy of Agricultural Sciences had a high curative effect on plant rot, and research showed that it could reach 80-100%. In 1971, the Jilin Academy of Agricultural Sciences isolated a new variant of Streptomyces nonhygroscopicus Gongzhuling from the local soil. Demons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00
Inventor 郑爱萍李平
Owner 成都川农邑邦农业发展有限公司
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