Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Time-resolved fluorescnet detection method and detector for gene chip

A technology of time-resolved fluorescence and gene chips, which is applied in the detection of gene chips and the field of special detection devices to realize this method, can solve the problems of low sensitivity, narrow analysis range, existence of impurities and noise, etc., and achieve long storage period and wide linear range , good stability

Inactive Publication Date: 2002-03-13
张添 +1
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the above-mentioned existing gene chip hybridization detection method is to use common fluorescent dye-labeled target molecules to hybridize with the spotted chip in a hybridization box in liquid phase, then dry and place it on the platform of the detector for detection or directly in the hybridization box After the reaction, the detection is cleaned, so the background noise is difficult to eliminate, so that the existing detection methods have problems such as low sensitivity, low hybridization efficiency, and narrow analysis range, which limits the promotion and application of biochip technology and the improvement of gene chip quality
Although the existing detectors have taken certain measures to eliminate background noise, such as using confocal scanning to reduce the fluorescence impurity noise on the substrate; using photomultiplier tubes instead of CCD to improve the extraction of fluorescence signals, etc., but the impurity noise still exists. Background noise is still not completely eliminated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Time-resolved fluorescnet detection method and detector for gene chip
  • Time-resolved fluorescnet detection method and detector for gene chip
  • Time-resolved fluorescnet detection method and detector for gene chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The steps to realize the gene chip time-resolved fluorescence detection method in this embodiment are: A. Immobilize the DNA probe array on the chip substrate, and mark it with the dye (such as europium, ruthenium ion and other chelates) bound by the rare earth element chelate The target molecule is hybridized in the hybridization box, and then the chip is cleaned, and the cleaned chip is placed on the detection platform; B. A nitrogen molecular laser with a light source wavelength of 337nm is used as the detection light source, and the light pulse width is 10ns after modulation. The light pulse repetition frequency is 15-20Hz, the incident light shines on the hybridization area of ​​the gene chip through the fluorescent lens, the excited dye emits 613nm fluorescence, enters the detection optical path through the lens with a high numerical aperture of 0.8 mm, and the gene chip emits light to the excitation light (337nm) also enters the detection optical path; C. Both the...

Embodiment 2

[0026] The steps of the time-resolved fluorescence detection method for the gene chip in this example differ from Example 1 in three main points: the hybridization reaction is carried out on the detector; beam-splitting optical path is used; and the delay time is short. Its concrete steps are as follows: A. DNA probe array is fixed on the chip substrate of spectral transparency with mechanical spotting, spray spot or in-situ synthesis method, and the material of chip adopts the LB film of polymer (also can be functionalized glass , Quartz, Si, Ge, GaAs, GaP, SiO 2 、Si 3 N 4 , modified Si gels, polymers, or gels such as polytetrafluoro, polyaluminum, polycarbonate, polymers, or their mixtures); the shape of the chip is a disc (also can be a square , round and other shapes); the surface of the chip adopts various microfabrication methods to produce the required surface features such as ribs, V-shaped grooves, and micro-holes; the hybridized area is closely connected with the f...

Embodiment 3

[0034] The difference between the present embodiment and the second embodiment is that the acquisition process is delayed by 800 microseconds, and the DNA probe array is first fixed on the spectrally transparent chip substrate, and then hybridized with the target molecule labeled with the rare earth ion chelate dye. Cartridge for hybridization reaction; after cleaning, place the chip on the support platform of the detection device; perform detection according to the steps after Example 1F. The device used is the same as in Example 1. Experiments have proved that the result is feasible.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the field of biological information testing technology. By using rare earth element ions with unique fluorescent characteristic as marker tracer and through time resolving fluorescent spectroscopic analysis to eliminate the effect of background fluorescence, the sensitivity of chip analysis is raised greatly. The detector of the present invention consists of frame, support platform, platform transmission mechanism, optical detection unit and photoelectronic signal processing unit. The present invention is superior to available technology obviously. The multifunctional instrument combining the temporal fluorescent resolving technology, gene chip detection technology and biological information technology has the advantages of high sensitivity, high stability, wide linearity range and long marker maintaining period.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of a gene chip. At the same time, the invention also relates to a special detection device for realizing the method, which belongs to the technical field of biological information detection. Background technique [0002] With the imminent completion of the Human Genome Project and the implementation of the Post-Genome Project, more and more genome sequences of animals, plants, and microorganisms have been determined, and gene sequence data is growing rapidly at an unprecedented rate. Therefore, it is particularly important to establish a new hybrid sequencing method for efficient and rapid detection and analysis of a large amount of genetic information. As a high-throughput detection method, gene chip has been vigorously developed. Although the prospect of gene chip technology is very bright, it should be seen that there are still many difficult problems on the way forwa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 陆祖宏朱纪军张添孙晓如陈扬
Owner 张添
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com