Microorganisms for treatment or prevention of obesity and diabetes mellitus, and pharmaceutical composition containing the same

A composition, microorganism technology, applied in the direction of drug combination, microorganism-based method, microorganism, etc., to achieve the effect of treating and preventing obesity and diabetes

Inactive Publication Date: 2002-11-20
BIONEER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, no single drug alone can treat or suppress obesity and diabetes at source

Method used

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  • Microorganisms for treatment or prevention of obesity and diabetes mellitus, and pharmaceutical composition containing the same
  • Microorganisms for treatment or prevention of obesity and diabetes mellitus, and pharmaceutical composition containing the same
  • Microorganisms for treatment or prevention of obesity and diabetes mellitus, and pharmaceutical composition containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] Selection of Exopolysaccharide-Producing Microorganisms from Samples

[0127] To isolate the microorganisms capable of producing edible fiber, samples were collected from sugar mill effluents and other locations. From the collected samples, 10 g of the mixture was disrupted and suspended in 90 ml of physiological saline (0.85% NaCl). The suspended sample was diluted to 10 with normal saline -2 , 10 -4 , 10 -6 . These diluted samples were then smeared on MRS agar medium (1% peptone, 1% beef extract, 0.5% yeast powder, 2% glucose, 0.1 % Tween-80, 0.2% Ammonium Citrate, 0.5% Sodium Acetate, 0.01% MgSO 4 , 0.005% MnSO 4 , 0.2% sodium phosphate pH6.5) and BSH agar medium (2% glucose, 0.5% peptone, 0.5% yeast powder, 0.27% Na 2 HPO 4, 0.115% citric acid pH 5.0) (Hestirin and Schramn, J. Gen. Microbiol., 11:123, 1954), cultured at 30°C for 72 hours. About 2000 colonies were isolated and first inoculated into 5ml of MRS liquid medium and BSH liquid medium for static cu...

Embodiment 3

[0141] Extent of intestinal digestive enzymes to degrade exopolysaccharides (edible fiber)

[0142] In order to determine whether the edible fiber produced by the microorganisms is degraded by intestinal digestive enzymes, 1 g of porcine pancreatic enzyme preparation (produced by sigma company) shows 3 times the activity of the United States Pharmacopoeia, which contains amylase, lipase, protease and nuclease, Suspend it in 1 g of dry dietary fiber buffer (pH 7.5). The suspension was cultured at 40° C. for 7 days, and the suspension was collected once a day, and the glucose in it was quantified using DNS (3,5-dinitrosalicylic acid). The measurement results showed that dietary fiber was not broken down at all.

[0143] Therefore, it can be confirmed that the dietary fiber produced by the microorganism of the present invention is not degraded in the intestinal tract.

Embodiment 4

[0145] Bacterial uptake rate of glucose

[0146] Lactobacillus acidophilus (Lactobacillus acidophilus) (KCTC3140) and Lactobacillus hilgardii (L.hilgardii) (KCTC3500) known as microecological regulator bacteria and said Lactobacillus (Lactobacillus) BC-Y009, Acetobacter (Acetobacter) BC-Y002 , the glucose absorption rate of Acetobacter BC-Y058 and Escherichia coli (E.coli) was determined under intestinal conditions. The result is expressed in figure 1 and Table 4.

[0147] Such as figure 1 As illustrated in Table 4, the microorganisms of the present invention are superior to other lactic acid bacteria in terms of glucose absorption rate.

[0148] Initial O.D.600nm

initial glucose concentration

glucose after one hour

O.D. Portugal per unit of time

(mM)

Concentration (mM)

Decrease in glucose concentration

(mM / hr / O.D.)

E. coli

3.0±0.1

110

85±0.5

8.3±0.44

BC-Y009

...

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Abstract

The present invention relates to microorganisms for the treatment or the prevention of obesity or diabetes mellitus, which reduce the amount of monosaccharide or disaccharide which may be absorbed into human body by converting monosaccharides such as glucose, fructose, galactose et al. and disaccharides into polymeric materials which cannot be absorbed by the intestine, and relates to a pharmaceutical composition containing the said microorganisms.

Description

technical field [0001] The present invention relates to microorganisms for the treatment or prevention of obesity and diabetes by converting monosaccharides such as glucose, fructose and galactose, etc. The amount of monosaccharides or disaccharides absorbed into the human body can be reduced, and the invention also relates to pharmaceutical compositions containing said microorganisms. Background technique [0002] As we all know, obesity has been reported as a chronic disease, many factors can lead to obesity, and its origin has not been studied clearly so far. Obesity is known as an inducing factor for a series of diseases, such as hypertension, diabetes, coronary heart disease, gallbladder disease, osteoarthritis, sleep apnea, respiratory disorders, endomerial, prostate cancer, breast cancer and colon cancer wait. [0003] According to the NIH report (The Evidence Report: clinical guideline on the identification, evaluation, and trentment of overweight and obesity inadu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61K35/741A61K35/744A61K35/747A61P3/04A61P3/10C12R1/02C12R1/225
CPCA61K35/74A61K35/742A61K35/744A61K35/745A61K35/747Y10S435/853Y10S435/823A61P3/04A61P3/10C12R2001/02C12N1/205C12R2001/225A61K2300/00
Inventor 朴翰浯方英培丁海重金峰徹金恒来
Owner BIONEER
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