Method of producing feed composite enzyme using orange peel as raw material by solid fermentation

A compound enzyme for feed and solid-state fermentation technology, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problem of low animal absorption rate, and achieve the effect of high enzyme activity, easy promotion, and low price

Inactive Publication Date: 2005-10-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Feed is generally dominated by by-products of agricultural product processing, and the absorption rate of animals is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Transfer the low-temperature-preserved Aspergillus slant strain to a fresh PDA slant, place it in a constant temperature incubator at a temperature of 28°C, and cultivate it for 84 hours. Wash the spores with an appropriate amount of sterile water to make a spore suspension and adjust the spore concentration to 10 7 pieces / ml. In a 500mL Erlenmeyer flask, put 40g of citrus peel solid fermentation medium, including: 15g of citrus peel powder, 5g of soybean meal, 0.2g of urea, 0.4g of ammonium sulfate, 20ml of water, sterilize at 121°C for 20min, and insert after cooling 2mL of spore suspension was placed in a biochemical incubator at 28°C for fermentation and culture. After 72 hours of fermentation, when the solid surface was covered with gray-black spores, add sterile water, wash the spores, and adjust the spore concentration to 10 7 pc / mL, spare. Spray 4L of the above-mentioned cultured spore suspension evenly into a multi-layer curved plate equipped with 100 kg of f...

Embodiment 2

[0034] Transfer the low-temperature-preserved Aspergillus slant strain to a fresh PDA slant, place it in a constant temperature incubator at a temperature of 28°C, and cultivate it for 84 hours. Wash the spores with an appropriate amount of sterile water to make a spore suspension and adjust the spore concentration to 10 7 pieces / ml. In each 500mL conical flask, put 40g of citrus peel solid fermentation medium, including: 16g of citrus peel powder, 4g of soybean meal, 0.3g of urea, 0.3g of ammonium sulfate, 20ml of water, sterilized at 121°C for 20min, after cooling Insert 2mL of spore suspension, put it in a biochemical incubator at 28°C for fermentation and culture, after 84 hours of cultivation, after the surface of the fermented solid is covered with gray-black spores, add sterile water, wash the spores, and adjust the concentration of spores to 107 / mL ,spare.

[0035]Spray 5L of the above-mentioned cultivated spore suspension evenly into a multi-layer curved plate equip...

Embodiment 3

[0038] Transfer the low-temperature-preserved Aspergillus Usami slant strain to a fresh PDA slant, place it in a constant temperature incubator at a temperature of 28°C, and cultivate it for 72 hours. Wash the spores with an appropriate amount of sterile water to make a spore suspension and adjust the spore concentration to 10 7 pieces / ml.

[0039] In a 500mL Erlenmeyer flask, put 45g of citrus peel solid fermentation medium, including: 16g of citrus peel powder, 4g of soybean meal, 0.3g of urea, 0.3g of ammonium sulfate, 25ml of water, sterilized at 121°C for 20min, and put in after cooling 2mL of spore suspension was placed in a biochemical incubator at 28°C for fermentation and cultivation. After 72 hours of fermentation, when the surface of the fermented solid was covered with gray-black spores, add sterile water to wash the spores, adjust the spore concentration to 107 / mL, and set aside.

[0040] Spray 5L of the above-mentioned cultivated spore suspension evenly into a m...

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Abstract

A process for preparing the composite enzyme for the feed includes such steps as mixing tangerine peel with soybean dregs and wheat bran, adding less inorganic salt and water, stirring, sterilizing, cooling, inoculating a kind of Aspergillus, solid fermenting and drying.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing compound enzymes for feed by solid-state fermentation using citrus peels as raw materials. Background technique [0002] With the development of citrus processing industry, a large amount of processing by-products will be produced, such as citrus peels and pressing residues, which account for about 25% to 40% of the whole fruit weight. Benefits and reduce pollution, protect the environment are of great significance. [0003] In recent decades, the energy problem has been one of the main social problems in the world. People are constantly looking for new energy sources, and the rational use of renewable resources is an important way to solve energy problems. The main way to solve the energy shortage in the feed industry is to improve the utilization rate of feed. Feed is generally dominated by by-products of agricultural product processing, and the absorption ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K20/189A23K50/75A23K50/80
CPCY02A40/818Y02P60/87
Inventor 郑晓冬单丽君叶兴乾
Owner ZHEJIANG UNIV
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