Barbadosnut salt induced transcription factor and its coding gene and uses

A transcription factor, salt-induced technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of plant stress resistance, plant stress resistance has not been comprehensively improved, single functional gene and other problems, to achieve high practical Application value, effect of improving stress resistance and broad application prospects

Inactive Publication Date: 2006-07-26
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of plant stress resistance traits, it is very difficult to improve plant stress resistance using traditional breeding methods. With the development of molecular biology, improving plant stress resistance through genetic processes has opened up a new way of plant stress resistance breeding. , but the isolation of high-efficiency stress-resistant genes has become the main factor limiting plant stress-resistant genetic engineering
In the past, single functional genes were mainly cloned and applied, such as betaine synthase gene and proline synthase gene, etc. Although some effects have been achieved, the stress resistance of plants has not been fully improved

Method used

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  • Barbadosnut salt induced transcription factor and its coding gene and uses
  • Barbadosnut salt induced transcription factor and its coding gene and uses
  • Barbadosnut salt induced transcription factor and its coding gene and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the acquisition of the complete cDNA sequence of the salt-induced transcription factor gene JcERF of Jatropha curcas

[0048] The acquisition of the full-length cDNA sequence of the Jatropha curcas salt-induced transcription factor gene JcERF comprises the following steps:

[0049] 1. Cloning of the 3' end sequence of the salt-induced transcription factor gene JcERF of Jatropha curcas

[0050] 1. Plant material processing and total RNA extraction

[0051] First take Jatropha curcas seedlings as material, extract total RNA after being treated with 300mM NaCl solution for 12 hours, and carry out 1% agarose gel electrophoresis detection to it, and the detection result is as follows: figure 1 As shown, the extracted total RNA has two obvious electrophoresis bands, which are 28s RNA and 18s RNA from top to bottom, indicating that the total RNA with high purity and completeness has been obtained.

[0052] 2. Cloning of the 3' end sequence of the salt-induced t...

Embodiment 2

[0069] Example 2, Bioinformatics analysis of JcERF and its encoded protein

[0070] 1. Sequence analysis of JcERF gene and prediction of structure and function of its encoded protein

[0071] Utilize DNAMAN software to carry out bioinformatics analysis to the full-length cDNA sequence of JcERF that embodiment 1 obtains, and its structural representation is as follows Figure 5 As shown (the single line indicates the 3'-UTR; the box indicates the open reading frame, which contains two functional motifs, the black box indicates the AP2 domain, and the hatched line on the right indicates the acidic activation region), the total length of the sequence is 759bp, from 5 The 1st-759th base at the 'end is ORF, which encodes a protein consisting of 253 amino acid residues. It is estimated that its molecular weight is 27.474kDa, and its isoelectric point pI value is 9.09. Using the SMART tool to predict the function of the deduced amino acid residue sequence, the result is that the pro...

Embodiment 3

[0076] Embodiment 3, the structural analysis of JcERF genomic DNA

[0077] Extract the genomic DNA of the Jatropha curcas seedling that is processed through 300mM NaCl solution in the embodiment 1 and take this as template, under the guidance of primer JcERFW-1 and and JcERFW-2, carry out the structure of PCR amplification analysis JcERF gene, reaction finishes Afterwards, the PCR products were detected by 1% agarose gel electrophoresis, and the detection results were as follows: Figure 9 As shown (swimming lane M is the molecular weight standard Marker III, and swimming lane 1 is the PCR amplification product), as a result, a specific band with a length of about 800 bp was amplified by PCR. Then use the cDNA of JcERF as a template, carry out PCR amplification with the above-mentioned same primers, after the reaction finishes, carry out 1% agarose gel electrophoresis detection to the PCR product, the detection result is as follows Figure 9 As shown (swimming lane M is the m...

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Abstract

This invention discloses a Jatropha curcas slat inducing transcription factor and its coding gene and application. The related enzyme is one the following amino acid remnant radical sequences: 1) SEQ ID íÝ: 1 in the sequence list; 2) the protein that can control the plant resistant reverse and replace, minus or add the acid remnant radical sequences of the SEQ I D íÝ: 1 in the sequence list through one to ten amino acid remnant radicals. Using this transferring gene technique and transplanting the gene in the plant host can obtain the transfer gene plant that has the improved aridity resist and high salt resist ability.

Description

technical field [0001] The present invention relates to a stress-related transcription factor and its coding gene and application in plants, in particular to a Jatropha curcas salt-induced transcription factor and its coding gene derived from the EREBP / AP2 family and its ability to improve stress resistance in cultivation application in plants. Background technique [0002] Plants in nature are often threatened by harsh environments such as drought, salinity, and low temperature, which inhibit their growth and development, and even cause plant death. Soil salinization is a major environmental stress factor affecting plant growth. According to statistics, saline-alkali land accounts for 7.5% of the earth's land area. At present, about one-third of the world's irrigable soil is no longer suitable for crop growth due to its high salt content. There are more than 1.5 billion mu of salinized land in China, mainly distributed in coastal, arid and semi-arid areas such as Shandong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
Inventor 唐明娟沈世华刘杰
Owner INST OF BOTANY CHINESE ACAD OF SCI
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