Novel extraction method of bromelain

A technology of bromelain and a new method, applied in directions such as hydrolase, can solve the problems of rough product, need to be improved in purity, large energy consumption, etc., and achieve the effects of improving purity and yield, shortening drying time, and improving use efficiency

Inactive Publication Date: 2007-02-07
谢丹青 +1
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical methods are mainly used for extraction in my country. The chemical methods are outdated, the products are rough, the content of impurities is high, and the enzyme activity is not high.
Patent CN96114240.5 provides a physical extraction method of bromelain. The raw material of pineapple plant is crushed and squeezed after being frozen and pretreated (the temperature is -5°C--20°C, and the time is 20-30 hours) (the pH of the juice should be adjusted to 4.0-4.7), after the juice is filtered and separated, use membrane technology for ultrafiltration and reverse osmosis to obtain purified and c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example one

[0023] The new method for physical extraction of bromelain in this embodiment includes the following steps: raw material → washing → coarse crushing → fine crushing → pretreatment → juice extraction → centrifugation → freezing → filtration → ultrafiltration 1 → ultrafiltration 2 → reverse osmosis → vacuum Drying → finished product. Specifically: using pineapple fruit, leaves, skins, and stems as raw materials, first flush the sediment on the raw materials with clean water, crush the raw materials into 5mm particles with a coarse crusher, and then crush them to 0.1mm particles with a fine crusher . Add 1% sodium metabisulfite as an antioxidant and 1% sodium benzoate as a bacteriostatic agent to the crushed fine particles, and then use a screw juicer to obtain the juice. The juice is then separated by a disc centrifuge, the supernatant is taken, and the residue Discarded. Then add 0.2% EDTA, 1% cysteine ​​and a small amount of filter aid to the supernatant, stir...

Example Embodiment

[0024] Example two

[0025] The new method for physical extraction of bromelain in this embodiment includes the following steps: raw material → washing → coarse crushing → fine crushing → pretreatment → juice extraction → centrifugation → freezing → filtration → ultrafiltration 1 → ultrafiltration 2 → vacuum drying → finished product . Specifically: using pineapple leaves, skins, and stems as raw materials, first wash the sediment on the raw materials with clean water, crush the raw materials into 10mm particles with a coarse crusher, and then crush them into 3mm particles with a fine crusher. Then add 2% sodium metabisulfite as an antioxidant and 5% sodium benzoate as a bacteriostatic agent to the fine particles, and then squeeze the juice with a hydraulic juicer to obtain the juice. The juice is separated by a three-foot centrifuge and the supernatant is taken. Discard the slag. Then add 0.5% EDTA, 0.5% cysteine ​​and a small amount of filter aid to the supernatant, stir well fo...

Example Embodiment

[0026] Example three

[0027] The new method for physical extraction of bromelain in this embodiment includes the following steps: raw material → washing → coarse crushing → fine crushing → pretreatment → juice extraction → centrifugation → freezing → filtration → ultrafiltration 1 → reverse osmosis → vacuum drying → finished product. Specifically: using pineapple leaves, skins, and stems as raw materials, first flush the sediment on the raw materials with clean water, crush the raw materials into 8mm particles with a coarse crusher, and then crush them into 1mm particles with a fine crusher. Then add 1.5% sodium metabisulfite as an antioxidant and 3% sodium benzoate as a bacteriostatic agent to the fine particles, then squeeze the juice with a rotary knife juicer, and then separate the juice with a horizontal continuous centrifuge, and take the supernatant Discard the slag. Then add 0.5% EDTA, 0.8% cysteine ​​and a small amount of filter aid to the supernatant, stir well for 40 m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

the invention discloses a new physical extracting method of pineapple proteinase, which comprises the following steps: cleaning raw material; grinding roughly; grinding finely; predisposing; squeezing; centrifuging; freezing; filtering through two-grade; proceeding two-grade hyperfiltration; hyperfiltrating; drying in the vacuum to obtain the product.

Description

technical field [0001] The invention relates to a method for extracting bromelain, in particular to a new method for extracting bromelain from pineapple plants using a physical method. Background technique [0002] Bromelain is a kind of proteolytic enzyme, a pure natural plant protease produced by biotechnology from the fruit, fruit stalk, peel, fruit leaf, stem leaf and stem of the pineapple plant. Bromelain is light gray to light yellow powder rich in pineapple aroma. Bromelain has a strong effect on decomposing fibrin and blood clots, and can effectively improve human immunity, so it has a significant medical effect. Bromelain can carry out various biochemical reactions such as protein hydrolysis, and bromelain can be widely used in food, biology, medicine, cosmetics and other industries. At present in my country, chemical methods are mainly used for extraction. The chemical methods are backward in technology, rough in product, high in impurity content, and low in enzy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/50
Inventor 刘锋
Owner 谢丹青
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap