Narrow bore layer open tube capillary column and uses thereof

a capillary column and narrow bore technology, applied in the direction of isotope separation, diaphragm, electrolysis, etc., can solve the problems of difficult preparation of monolithic columns, low retention and sample loading capacity, and difficult lc-ms analysis of very small quantity samples, etc., to achieve high column-to-column reproducibility, high resolving power, and high resolving power
US20090203146A1Inactive Publication Date: 2009-08-13NORTHEASTERN UNIV
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
NORTHEASTERN UNIV
Publication Date
2009-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

A polymer-based PLOT column prepared by in situ copolymerization of a functional monomer, which usually contains the retentive chemistries, and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Styrenic based monomers such as styrene and divinylbenzene or meth / acrylic based monomers such as butyl or stearyl methacrylate and ethylene glycol dimethacrylate, are preferred. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 μm. Thus, they are suitable for commercial use in ultratrace LC / MS proteomic analysis. Columns according to the invention are characterized by high resolving power, high column-to-column reproducibility and relatively high loading capacity. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems provide high sensitivity for analysis of complex proteomic samples, even down to the low attomole to sub-attomole level.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit under 35 U.S.C. § 119(e) of U.S. Provisional Patent Application No. 60 / 815,314, filed on Jun. 21, 2006, the disclosure of which is incorporated by reference herein.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0002] Part of the work leading to this invention was carried out with United States Government support provided under a grant from the National Institutes of Health, Grant No. GM-15847. Therefore, the U.S. Government has certain rights in this invention.BACKGROUND OF THE INVENTION

[0003] Electrospray ionization-mass spectrometry (ESI-MS) has become a routine tool in proteomic studies, primarily due to its high sensitivity, broad dynamic range, and versatility for online coupling with capillary high performance liquid chromatography (HPLC)1-3. High-resolution separation prior to MS detection allows complex mixtures to be characterized by extending both the dynamic range and detection...

Claims

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