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Biological active coating components, coatings, and coated surfaces

a technology of bioactive coatings and components, applied in the field of bioactive coating components, coatings, and coated surfaces, can solve problems such as surface bioactive, and achieve the effects of minimizing logistical requirements, easy application, and enhancing the life time of biomolecule composition

Inactive Publication Date: 2010-09-30
REACTIVE SURFACES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0024]In light of these and additional disclosures herein, it is now possible to produce paints and other coatings that detoxify chemical compounds for extended periods. Remarkably, a preferred enzyme composition of the present invention remained stable in the coating for an extended period of time (e.g., months) at ambient conditions. It is contemplated that the extended period of activity may further comprise time periods in excess of a year. In particular, stabilized embodiments of the enzyme composition are designed to enhance the life time of the biomolecule composition in a coating and on a surface. In a preferred embodiment, the invention comprises at least one polymer-based compound that provides prophylactic protection and continuous detoxification of an organophosphorus nerve agent.
[0025]In certain embodiments, it is contemplated that the compositions and methods of the present invention may be used to produce self-decontaminating surfaces that remain active for extended periods. In a specific aspect, it is contemplated that such a self-decontaminating surface will not need additional decontamination compositions or methods to effect decontamination, thereby minimizing logistical requirement. In a particular aspect, it is contemplated that the compositions and methods of the present invention may be easily applied in advance of, during or after exposure to a chemical compound. In another preferred aspect, the compositions and methods of the present invention will be used in conjunction with existing other decontamination compositions and methods. In a preferred facet, the compositions and methods of the present invention are applied in advance of, during or after a field-based military operation to protect troops against a chemical warfare agent.
[0031]A further disclosure of the present invention is the preparation of an active biomolecule with a limited number of processing and / or purification steps from other cellular molecules of the organism in which it was produced. Typically, the organism is a microorganism. The combination of an active biomolecule and such cellular molecules is known herein as a “biomolecule composition”. It is contemplated that the inclusion of such cellular molecules may provide a protective or stabilizing effect for the activity of the active biomolecule. An example would be the protective encapsulation of an active biomolecule by a cell wall and / or cell membrane of the organism. Such additional cellular molecules contribute to the particulate nature of a biomolecule composition, which may grant utility for such material as a particulate component of a coating or paint, such as a pigment, an dispersant, a rheological agent, or a combination thereof.

Problems solved by technology

In addition, it still retains activity after the paint is applied to a surface, and renders the surface bioactive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Assay for Active Phosphoric Triester Hydrolase Expression in Cells

[0789]Routine analysis of parathion hydrolysis in whole cells is accomplished by suspending cultures in 10 milli-Molar (“mM”) Tris hydrocholoride at pH 8.0 comprising 1.0 mM sodium EDTA (“TE buffer”). Cell-free extracts are assayed using sonicated extracts in 0.5 milliLiters (“ml”) of TE buffer. The suspended cells or cell extracts are incubated with 10 microLiters (“μl”) of substrate, specifically 100 μg of parathion in 10% methanol, and p-nitrophenol production is monitored at a wavelength of 400 nm. To induce the opd gene under lac control, 1.0 μmol of isopropyl-β-D-thiogalactopyranoside (Sigma) per ml is added to the culture media.

example 2

Preparation of Enzyme Powder

[0790]In a typical preparation, a single colony of bacteria that expresses the opd gene is selected and cultured in a rich media. After growth to saturation, the cells are concentrated by centrifugation at 7000 rotations per minute (“rpm”) for 10 minutes for example. The cell pellet is then resuspended in a volatile organic solvent such as acetone one or two times in order to dessicate the cells and to remove a substantial portion of the water contained in the cell pellet. The pellet may then be ground or milled to a powder form. The powder may be frozen or stored at ambient conditions for future use, or may be added immediately to a surface coating formulation. Additionally, the powder may be freeze dried, combined with a cryoprotectant (e.g., cryopreservative), or a combination thereof.

example 3

Two-Pack OPH Paint Coating: OPH Powder and Latex Paint

[0791]In an example of use of the powder prepared as described in Example 2, 3 mg of the milled powder was added to 3 ml of 50% glycerol. The suspension was then added to 100 ml of Olympic® premium interior flat latex paint (Olympic®, One PPG Place, Pittsburg, Pa. 15272 USA). This paint with biomolecular composition was then used to demonstrate the activity of the paint biomolecular composition in hydrolysis of a pesticide or a nerve agent analog.

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PUM

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Abstract

Disclosed herein are novel coatings and paints comprising a biomolecule composition, wherein the biomolecule composition comprises a phosphoric triester hydrolase. Also disclosed herein are methods of detoxification of a surface contaminated with an organophosphorus compound by contacting the surface with such a coating or paint. Also disclosed herein are novel coating and paint components derived from microorganisms.

Description

[0001]This application is a divisional application of U.S. Ser. No. 10 / 655,345 filed Sep. 4, 2003, claims the benefit of Provisional Patent Application Entitled “Bioactive Protein Paint Additive, Paint, and Painted Various,” Ser. No. 60 / 409,102, filed Sep. 9, 2002, which are incorporated herein in their entirety by reference.BACKGROUND OF THE INVENTION[0002]A. Field of the Invention[0003]The present invention relates generally to the field of biological molecules as components of coatings conferring an activity or other advantage to the coating proteinaceous molecule related to the biological molecule. More specifically, the present invention relates to proteins as such components of coatings. In one specific regard, the present invention relates to protein compositions capable of organophosphorus detoxification, and methods of reducing organophosphorus compounds on surfaces. More specifically, the present invention relates to coatings such as paints that degrade organophosphorus co...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A62D3/02A01N63/00A61F2/00A61K38/46A61K38/48A61K39/00A61K47/48A62D101/02A62D101/45C09D5/00C09D7/48C09J11/02C12M1/34C12N9/00C12N9/14C12N9/88C12N9/90C12N11/08C12S9/00
CPCA62D3/02A62D2101/02A62D2101/26C09D5/00C12N11/16C09D7/1233C09J11/02C12N9/16C12N11/08C09D5/34C09D7/48A61K47/50C09D7/65C12N9/00C12N9/14C12N9/88C12N9/90A62D5/00
Inventor MCDANIEL, C. STEVEN
Owner REACTIVE SURFACES
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