Fructose-1,6-diphosphate calcium preparation and uses
A technology of diphosphoric acid and fructose diphosphate, applied in the field of fructose-1, can solve the problems of poor absorption, difficult absorption, large dosage, etc.
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Embodiment 1
[0050] Configure 58L of FDP solution (concentration 86g / L), adjust pH to 5.0. Add 2L CaCl 2 The solution, the molar concentration of 7.2mol / L, was stirred at 250rpm for 30min, concentrated in vacuo, slowly added 2 times the volume of the solution to obtain alcohol, stirred for 30min, vacuum filtered, washed with a small amount of 80% alcohol water, and then washed with anhydrous The alcohol was dehydrated twice, vacuum filtered, and spray-dried to obtain 5.1 kg of fructose-1,6-diphosphate monocalcium salt with a purity of 99.0% and a yield of 95% of the theoretical value.
Embodiment 2
[0052] Prepare 50L of FDP solution (concentration is 34.5g / L), adjust pH to 2.0. Add 5L Ca(NO 3 ) 2 (concentration is 5.04mol / L), under the condition of 250rpm, stirring reaction for 4h, vacuum concentration, adding ethanol of twice the solution volume, and stirring for 15min, after fructose-1,6-diphosphate monocalcium salt crystallizes, vacuum filtration , washed with a small amount of 80% alcohol water, then dehydrated twice with anhydrous alcohol, vacuum filtered, crystallized in 3 times alcohol, freeze-dried fructose-1,6-diphosphate monocalcium salt 1.76kg, purity 99.0 %, the yield is 96% of the theoretical value.
Embodiment 3
[0054] Prepare 5L of FDP solution (concentration is 92g / L), adjust pH to 6.0 and add 500ml CaCl 2 The solution (concentration is 2.7mol / L), stirred and reacted for 4h, added 2.5 volumes of acetone, and stirred for 15min, vacuum filtered after crystallization, washed with a small amount of 80% alcohol water, then dehydrated with anhydrous alcohol for 2 times, vacuumed After suction filtration, the spray-dried fructose-1,6-diphosphate monocalcium salt was 501.1 g, the purity was 99.0%, and the yield was 95% of the theoretical value.
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