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Method for improving chemical stability of biological collagen to antibiotic medicine

A technology of chemical stability and collagen, applied in the direction of drug combination, digestive system, inactive components of polymer compounds, etc., to achieve good biodegradability, improve technology content, and expand market share.

Inactive Publication Date: 2009-12-16
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This characteristic is that the contact mode of collagen and human body is different from other polymers

Method used

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  • Method for improving chemical stability of biological collagen to antibiotic medicine
  • Method for improving chemical stability of biological collagen to antibiotic medicine
  • Method for improving chemical stability of biological collagen to antibiotic medicine

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Example 1: Using NH 4 Cl-pepsin-NaHCO 3 Modulated O / W system

[0033] Step 1) Modulation of soluble collagen:

[0034] Cow split hide is made into gray hide, weighed 10g, and broken. Add liming agent Ca(OH) with a concentration of 3% by mass 2 , water 100ml, liming, after reacting at room temperature for 24 hours, fully washed with water, and then deliming agent NH with a concentration of 6% by mass 4 Cl, 50ml of water, deashed, reacted at room temperature for 6h; fully washed with water; minced; hydrolyzed with pepsin at a mass percentage concentration of 3‰, 4°C for 72h; centrifuged at a speed of 15000rpm; Salt out with 4% NaCl by mass, let it stand for 24 hours; centrifuge twice at 15,000 rpm; use 0.5mol / L acetic acid 200ml for acid dissolution to obtain soluble collagen, and 0.9g collagen for 10g gray skin.

[0035] The principle of enzymatic hydrolysis to extract collagen is as follows: figure 1 As shown: (a) is the original state of procollagen, with a regul...

example 2

[0042] Example 2: Using NH 4 O / W system modulated by Cl-pepsin-NaOH

[0043] Step 1) Modulation of soluble collagen:

[0044] Cow split hide is made into gray hide, weighed 10g, and broken. Add liming agent Ca(OH) with a concentration of 2% by mass 2 , water 100ml, liming, after reacting at room temperature for 48 hours, fully washed with water, and then using 4% deliming agent NH 4 Cl, 50ml of water, deashed, reacted at room temperature for 10h; fully washed with water; minced; hydrolyzed with pepsin at a mass percentage concentration of 2‰, 4°C for 72h; centrifuged at a speed of 15000rpm; adjusted to pH 7 with NaOH, using Salt out with 4% NaCl by mass percentage, let stand for 48 hours; centrifuge twice at 15,000 rpm; use 200 ml of 0.5 mol / L acetic acid for acid dissolution to obtain soluble collagen.

[0045] Step 2) Collagen modification:

[0046] Adjust the soluble collagen solution to pH 7 with NaOH, add succinic anhydride to make the solution pH = 4, react at room ...

example 3

[0049] Example 3: Using (NH 4 ) 2 SO 4 - Pepsin - NaHCO 3 Modulated O / W system

[0050] Step 1) Modulation of soluble collagen:

[0051] Cow split hide is made into gray hide, weighed 10g, and broken. Add liming agent Ca(OH) with a concentration of 4% by mass 2 , water 100ml, liming, after reacting at room temperature for 24 hours, fully washed with water, and then deliming agent (NH 4 ) 2 SO 4 , water 50ml, carry out deliming, after reacting at room temperature for 5 hours, fully wash with water; grind; hydrolyze with pepsin with a mass percentage concentration of 5‰, 4°C for 36 hours; centrifuge at a speed of 20000rpm; adjust the pH value to 7 with NaOH Salt out with 4% NaCl by mass percentage, let stand for 36 hours; centrifuge twice at 20,000 rpm; use 200 ml of 0.5 mol / L acetic acid for acid dissolution to obtain soluble collagen. Compared with Example 1, the same soluble collagen is obtained, the preparation process is shortened by 25h, and the industrial cost is...

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Abstract

The present invention provides a method for biological collagen to improve chemical stability of antibiotic medicine, which belongs to medicament field. Hydrochloride Minocycline antibiotics is stabile in low PH value, but its structure contained '=O' chemical bond, so easy react shift-base with '-NH2' group. Said reaction easy occurs when collagen containing amphoteric protein with '-NH2', '-COOH' group as carrier. The method provided by the invention includes steps: collagen is hydrolytic made into solubility collagen by low temperature enzyme method; collagen is amido chemical modified with anhydride in solution, '-NH2' group of collagen is beclouded, collagen structure becomes W phase by generating R-OOH from H2N-R-COOH; hydrochloride minocycline is mixed with oil, and medicine coated with oil increases chemical reaction difficulty with collagen and becomes O phase; thickening agent is added into modified collagen W phase, and is made into low PH value O / W homogeneous solution with oil mixtures hydrochloride minocycline. The method enhances chemical stability of biological collagen to hydrochloride minocycline antibiotics by using collagen amido-base modified, and is hopeful used in biological medical material.

Description

technical field [0001] The invention uses bovine collagen and modifies it to improve the chemical stability of the antibiotic minocycline hydrochloride, which is suitable for a drug slow-release system in stomatology and belongs to the field of pharmacy. Background technique [0002] The drug sustained release system is a controlled release drug delivery system (Controled Releases Drug Delivery System), referred to as CRD. CRD has its advantages over traditional dosage forms. [0003] Periodontitis is a common oral disease with an incidence rate of 70-80%. Topical administration of periodontal medicine is an important item in periodontal treatment. At present, there are many kinds of local effective sustained-release agents for periodontitis. Synthetic polymeric carrier materials are receiving increasing attention. Such as semi-synthetic polymers, commonly used are cellulose derivatives, such as methyl cellulose ether. Synthetic polymer materials, commonly used are poly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K9/107A61K31/65C12P21/02A61P1/02
Inventor 张文熊長南康正刘晶冰武芳马明霞
Owner BEIJING UNIV OF TECH