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Collagen modified poly(3-hydroxybutyrate-3-hydroxyvalerate) and its preparing method and application

A technology of hydroxybutyrate and hydroxyvalerate, applied in the field of collagen modified polymer and its preparation, can solve the problem of unfavorable cell-specific adhesion and specific gene activation, degradable polymer materials do not contain biological information, and lack cell recognition Signal and other issues, to achieve the effect of improving cell adhesion rate, good biocompatibility, and simple process

Active Publication Date: 2007-08-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, degradable polymer materials do not contain biological information, lack cell recognition signals, and are not conducive to cell-specific adhesion and activation of specific genes.

Method used

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  • Collagen modified poly(3-hydroxybutyrate-3-hydroxyvalerate) and its preparing method and application
  • Collagen modified poly(3-hydroxybutyrate-3-hydroxyvalerate) and its preparing method and application
  • Collagen modified poly(3-hydroxybutyrate-3-hydroxyvalerate) and its preparing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The process of preparing the collagen-modified PHBV material includes the following steps: (1) dissolving 0.06g PHBV in 1ml chloroform solution, and pouring the solution to prepare a PHBV film; (2) soaking the PHBV film prepared in step (1) in a concentration of 0.5% The initiator benzophenone acetone solution 4hr; (3) immerse the PHBV film soaked in the initiator in the aqueous solution of 5% acrylamide, and irradiate with ultraviolet light for 0.5hr; (4) extract with acetone for 48hr, soak in distilled water And dry at 60 DEG C; (5) add 10ml concentration and be the mixed alkaline solution of 5% sodium hydroxide and 6% sodium hypochlorite, wash with water after soaking for 20 minutes; (6) Add 5ml formaldehyde and wash with water after soaking at room temperature; (7) ) adding 10 ml of bovine collagen type I solution with a concentration of 3 mg / ml, standing at room temperature for 6 hours, and then rinsing with water; (8) freeze-drying at -50° C. for 12 hours to obtain...

Embodiment 2

[0048] The process of preparing the collagen-modified PHBV material includes the following steps: (1) dissolving 0.06g PHBV in 0.3ml dichloromethane solution, and pouring the solution to prepare a PHBV film; (2) soaking the PHBV film prepared in step (1) in a concentration of (3) immerse the PHBV membrane soaked in the initiator in an aqueous solution of 30% acrylamide, and irradiate with ultraviolet light for 4hr; (4) extract with acetone for 48hr, soak in distilled water Wash and dry at 60°C; (5) add 30ml concentration of 20% sodium hydroxide and 24% sodium hypochlorite mixed alkali solution, soak for 60 minutes and wash with water; (6) add 30ml formaldehyde for room temperature soak and wash with water; ( 7) Add 30 ml of bovine collagen type I solution with a concentration of 6 mg / ml and place at room temperature for 24 hours, then soak in water; (8) Freeze-dry at -20°C for 24 hours to obtain the modified material of the present invention.

[0049] 6 and 7 are scanning elec...

Embodiment 3

[0055] The process of preparing the collagen-modified PHBV material includes the following steps: (1) dissolving 0.06g PHBV in 0.6ml propylene carbonate solution, and pouring the solution to prepare a PHBV film; (2) soaking the PHBV film prepared in step (1) in The concentration is 1% initiator thioxanthone acetone solution for 8hr; (3) immersing the PHBV membrane soaked in the initiator in the aqueous solution of 10% acrylamide, and irradiating with ultraviolet light for 1hr; (4) extracting with acetone for 48hr, Rinse with distilled water and dry at 60°C; (5) add 15ml of a mixed alkali solution of 10% sodium hydroxide and 12% sodium hypobromite, soak for 30 minutes and wash with water; (6) add 10ml of pentadiene (7) Add 20ml of bovine collagen type I solution with a concentration of 3mg / ml and leave it at room temperature for 12 hours, then wash with water; (8) Freeze-dry at -50°C for 24 hours to obtain the modified material of the present invention.

[0056] The layered str...

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Abstract

The invention discloses a modified collagen with 5-30% collagen, 50-90% poly-3-hydrobutanoate-3-hydrovalerate and 5-20% partial amidifying polyacrylamide and making method and application, which comprises the following steps: adopting PHBV as basic material to make PHBV film; irradiating through ultraviolet; grafting polyacrylamide; making amidified PHBV to do Hofmann decomposing reaction; producing amidifying PHBV; bonding PHBV and collagen with excellent compatibility and dynamics.

Description

technical field [0001] The invention relates to a modified biological material and its preparation technology, in particular to a collagen-modified poly(3-hydroxybutyrate-3-hydroxyvalerate) with good biocompatibility and mechanical properties and its Preparation. Background technique [0002] Poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) is a natural polyester polymer synthesized by microorganisms. Due to its good biocompatibility, degradability and piezoelectricity, it has broad application prospects in the fields of drug controlled release, suture, artificial skin, cartilage and bone tissue engineering. However, PHBV is poorly hydrophilic, and cells adhere weakly to its surface. For this reason, the introduction of functional groups on the surface of PHBV, thereby improving the hydrophilicity and biological activity of the surface of PHBV, and then improving the interaction between cells and materials has always been a hot spot in the functionalization of biomedical m...

Claims

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Application Information

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IPC IPC(8): C08G63/91C08G81/00C08J7/18C08K5/07A61K47/34A61L27/06A61L27/24A61L24/10A61K47/42
Inventor 王迎军柯渔任力
Owner SOUTH CHINA UNIV OF TECH
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