Method for determining glucose and 1,5-anhydroglucitol in identicial colorimetric cell

A technology of anhydroglucitol and glucose, which is applied in the field of life science-clinical chemistry, can solve the problem of high measurement cost and achieve the effect of reducing detection cost

Inactive Publication Date: 2007-11-14
冯景
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  • Application Information

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Problems solved by technology

These two substances are routine indicators for the detection of diabetes in foreign countries, and the cost of separate determination is very high. For example, the interference of serum glucose must be removed for the determination of 1,5-AG by enzymatic method (and there is a waste of not measuring glucose)

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  • Method for determining glucose and 1,5-anhydroglucitol in identicial colorimetric cell
  • Method for determining glucose and 1,5-anhydroglucitol in identicial colorimetric cell
  • Method for determining glucose and 1,5-anhydroglucitol in identicial colorimetric cell

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Embodiment Construction

[0006] Materials and Methods

[0007] 1. Reagents

[0008] (1) Reagent I (RI) Tris hydrochloric acid buffer solution (Tris / HCl) contains glucose-6-phosphate dehydrogenase (G-6-PD) and horseradish peroxidase (HRP). (2) Reagent II (RII) N-2-hydroxyethylpiperazine N'-2-ethanesulfonic acid solution (HEPES) contains furanose oxidase (PROD), ascorbate oxidase (AO), 3-hydroxy - 2,4,6-triiodobenzoic acid (HTIB,). (3) Standard solution 50mmol / L Glu standard solution and 1mmol / L 1,5-AG standard solution.

[0009] 2. Instrument RA1000 (Technicon, USA), Hitachi 7080 automatic biochemical analyzer (Hitachi, Japan).

[0010] 3. Method (1) Principle of the method Firstly, the serum is treated with RI, and the Glu in the serum is completely converted into 6-phosphate that does not react with PROD through the coupling system of GK and glucose-6-phosphate dehydrogenase (G-6-PD) Gluconolactone (6-PGA) to remove the interference of endogenous Glu and measure the change in absorbance to calcul...

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Abstract

This invention discloses a method to measure the same comparing color pool of glucose and 1,5-glucose alcohol dehydration. First, the buffer solution composed by 3-hydroxymethyl-methane and hydrochloric acid is used to deal with serum. Through the GK and glucose -6 -- phosphate dehydrogenase coupling system glucose can be completely converted in serum into the 6 - phosphorylation gluconic acid lactone which not responds to PROD, to remove the interference of endogenous glucose, and determine the absorption of light to detect glucose content. Then the concentration of 200 mmol / L N-2-hydroxyethyl piperazine N'-2 - ethane sulfonic acid solution is added, and the tested 1,5-dehydrated alcohol is oxide into 1,5 - dehydration fructose and H2O2. The new zoology oxygen that H2O2 releases will react with HRP, 4-AAP HTIB response system and after Trinders role color reaction (from colorless to red), colorimetric determinate of 1,5 - dehydration glucose alcohol content is detected. This invention measure in the same comparing color pool of glucose and 1,5 - alcohol dehydration glucose, is sensitive, accurate, stable, specific characteristics and can reduce the cost of testing, and provide a new method for the detection and treatment of diabetes monitoring.

Description

technical field [0001] The invention belongs to the field of life science-clinical chemistry and relates to the determination of glucose and 1,5-anhydroglucitol in the same colorimetric cell. Background technique [0002] The sugar alcohols in serum are mainly glucose (Glu) and 1,5-anhydroglucitol (1,5-AG). 1,5-AG is the C1-deoxygenated form of glucofuranose, which is mainly derived from food. It exists in human cerebrospinal fluid and serum, forming a stable storage pool in the body. Its content in serum is second only to polysaccharide alcohols in Glu. With the increase of blood glucose concentration in diabetes, the ability of renal tubules to reabsorb 1,5-AG decreases, resulting in a significant decrease in the content of 1,5-AG in serum. Studies have shown that serum 1,5-AG detection as a randomized test for screening diabetes is more sensitive than other evaluation tests. However, although there are many methods for the determination of 1,5-AG, such as chromatograph...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/00G01N33/66C12Q1/54
Inventor 冯景
Owner 冯景
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