Method for preparing non-virus cation type gene vector

Abstract

The invention relates to a novel non-virus cationic gene vector and the process for preparation. The process for preparation comprises the following steps: firstly, utilizing an oxygen anion polymerization method to synthesize polycation type electrolyte and segmented copolymer which contains polycation type electrolyte, and then leading polycation type electrolyte and DNA to mutually compound in salting liquid whose PH is 7.4 and buffer solution, achieving the packing of polycation type electrolyte to DNA, and the surface of DNA / polycation compound which is formed is with positive charge, adding segmented copolymer MePEO-b-PMAA which contains polycation type electrolyte in a system, leading PMAA segment with negative charge to be absorbed on the surface of the DNA / polycation compound through electrostatic force action and hydrogen bond effect, achieving the packing to compounds. The novel non-virus cationic gene vector has the advantages of mild polymerization reaction condition, controllable molecular weight, narrow molecular weight distribution, simple process for preparing gene vector, high stability and bioavailability, low toxicity and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical polymer materials, in particular to a preparation method of a novel non-viral cationic gene carrier. Background technique [0002] Gene therapy is a new treatment method that has important potential therapeutic effects on various gene-related diseases that threaten human life. Gene therapy has made many amazing achievements and encountered many difficulties in the development path of more than 40 years. In order for gene therapy to truly enter clinical application, there are still some fundamental problems that have not been resolved, the most important of which is the construction of a safe, efficient and controllable gene carrier. [0003] At present, there are mainly three different gene delivery systems in the field of gene therapy: (1) physical transfection technology systems, such as electroporation and particle bombardment technology, etc. relatively low trans...

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Problems solved by technology

[0003] At present, there are mainly three different gene delivery systems in the field of gene therapy: (1) physical transfection technology systems, such as electroporation and particle bombardment technology, etc. relatively low transfection efficiency

(2) Viral vectors, including retroviruses, adenoviruses, and adeno-associated viruses, have shown high transduction efficiency in vitro; however, most viral vectors need to retain the outer shell and infection mechanism of wild viruses , there are some hidden dangers in terms of safety, such as the immune response to the carrier shell, the carcinogenicity of random gene integration, and potential endogenous virus recombination; at the same time, the viral vector lacks a targeting mechanism in the body, and its controllability is relatively poor. For example, in tumor gene therapy, the viral vector tumor gene therapy program is mainly aimed at some superficial and accessible tumors, such as skin cancer, head and neck tumors, etc., by intratumoral or peritumoral injection

This embedding method is simple and solves the compatibility problem of organisms, but at the same time, the in vitro cell transfection shows that its transfection efficiency is reduced

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