Colophony based functional polymer immobilization diastase and preparation method thereof

Abstract

The invention discloses a rosinyl functional polymer immobilized amylase and a preparation method thereof. The amylase is a maleated rosin glycol acrylic ester-acrylic acid copolymer immobilized amylase prepared from a maleated rosin glycol acrylic ester-acrylic acid copolymer and an amylase. The activity is between 20 and 30mg.g<-1>.5min<-1>, the residual activity after being repeated use for thirteen times is not lower than 6 mg.g<-1>.5min<-1>, and the relatively retained activity is not lower than 20 percent. The invention also discloses the preparation method for the immobilized amylase. The immobilized amylase can be used for catalyzing the starch to be hydrolyzed to generate dextrin and reducing sugar, and has wide application in the food, light industry and medicine fields.

Description

technical field

[0001] The invention relates to a rosin-based functional macromolecule immobilized amylase and a preparation method thereof, more specifically the invention relates to a maleic rosin ethylene glycol acrylate-acrylic acid copolymer immobilized amylase and a preparation method thereof. Background technique

[0002] As a biocatalyst, enzymes have the advantages of high catalytic efficiency, strong specificity, mild reaction conditions, biodegradability and no environmental pollution; however, enzymes are very sensitive to environmental influences, and various chemical, physical and biological factors (including Temperature, pressure, organic solvents, ionic strength, pH, etc.) can all cause enzymes to lose their biological activity. Therefore, the research on immobilized enzymes has been paid attention to. Immobilized enzymes have catalytic properties, have the advantages of being recyclable and reusable, and the production process can be continuous and automate...

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