Preparation method for one-size nano-particle fluorescence microsphere

A technology of nanoparticles and fluorescent microspheres, applied in the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., to achieve the effect of improving the degree of aggregation, the coupling between particles is basically consistent, and the luminous intensity is high
CN101342472BInactive Publication Date: 2011-02-09SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2011-02-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a preparation method of an uniform-size nanoparticle fluorescent microsphere. The density of fluorescent nanoparticle colloidal aqueous solution of the microsphere is adjusted to the micro-mol level so as to obtain the colloidal solution evenly scattered in the aqueous solution; the evenly scattered colloidal solution in the aqueous solution is injected into oil-phase solvent through capillary at the speed of 0.5 to 1.25 percent Vs / min so as to form droplets and Vs is the volume of the oil-phase solvent; the oil-phase solvent adopts bi- or multi-mixture with thesolubility between 2 to 10 percent and the density equal to the density of water; containers and reflux pipes adopt the hydrophobic material; the water in droplets is absorbed by the oil phase and the nanoparticles form a dense sphere; an absorbent continuously absorbs the water dissolved in the oil phase, so that the water content in the oil phase is far lower than the saturation; the nanoparticle polymer with the size from dozens of nano to hundreds of nano, the spherical height higher than 5 percent and the size error less than 10 percent is obtained through regulating the density of the quantum pot colloidal solution and the velocity of the reflux pipe.
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Description

technical field

[0001] The invention relates to controlling the size of nanoparticle aggregates by controlling the formation of microemulsions to achieve the preparation of fluorescent microspheres with uniform size, and belongs to the technical field of preparation of nanoscale nanoparticle aggregates. Background technique

[0002] The unique fluorescent properties of nanoparticles can be used as biomarkers. The existing method is to coat the surface of nanoparticles with conjugated molecules with biospecific groups through chemical modification to achieve the labeling of different biological substances. However, due to a single semiconductor The fluorescence intensity of quantum dots is weak, and it is difficult to realize the identification of individual particles, and the surface modification of individual particles cannot effectively eliminate the non-specific binding caused by physical adsorption on the surface of small particles (<10 nanometers). Pack multiple nan...

Claims

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