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Preparation method of nano-hydroxyapatite/chitosan scaffold with oriented particle arrangement

A nano-hydroxyapatite, directional arrangement technology, applied in medical science, prosthesis, bone implants, etc., can solve the problem that the nano-hydroxyapatite and chitosan cannot be effectively combined and cannot form an effective bond. , limiting functional applications and other issues, to achieve the effects of good binding force and compatibility, controllable crystallinity, and controllable crystallinity

Inactive Publication Date: 2012-02-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although the above method improves the activity and flexibility of a single chitosan material to a certain extent, because hydroxyapatite is compounded with chitosan and other matrices in powder form, hydroxyapatite is easy to agglomerate, in fact It is difficult to disperse evenly and cannot form an effective bond with the chitosan matrix. The hydroxyapatite particles can only be loosely distributed in the chitosan framework, which cannot effectively realize the interaction between nano-hydroxyapatite and chitosan. It is also difficult to really solve the main problems of chitosan materials, thus limiting its function and application in the field of biomedicine

Method used

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  • Preparation method of nano-hydroxyapatite/chitosan scaffold with oriented particle arrangement
  • Preparation method of nano-hydroxyapatite/chitosan scaffold with oriented particle arrangement

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Experimental program
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Effect test

Embodiment 1

[0044] A method for preparing a nano-hydroxyapatite / chitosan scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0045] Step 1: Preparation of nano-hydroxyapatite sol

[0046](1) Use polyvinylpyrrolidone (PVP K15) as template agent, dispersant and modifier at the same time;

[0047] (2) Use deionized water to prepare a solution containing 0.01mol / L of calcium nitrate and 0.006mol / L of diammonium hydrogen phosphate, and then add 0.1g of PVP K15 to the above calcium nitrate solution to fully dissolve;

[0048] (3) adopting a high-speed disperser at 100 rev / min, under the condition that the pH value of the ammonia water control system is 10.6, the materials in the above (1) and (2) are fully mixed to form nano-hydroxyapatite (HA) sol, The molar ratio of calcium to phosphorus in the nano-hydroxyapatite sol system reaches 1.6:1, and the polyvinylpyrrolidone content is 0.01% by mass percentage;

[0049] Step 2: Aging of nano-...

Embodiment 2

[0059] A method for preparing a nano-hydroxyapatite / chitosan scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0060] Step 1: Preparation of nano-hydroxyapatite sol

[0061] (1) Use polyvinylpyrrolidone (PVP K30) as template agent, dispersant and modifier at the same time;

[0062] (2) Use the PVP K30 aqueous solution of mass percent 0.5% to prepare the solution containing calcium nitrate 0.1mol / L, disodium hydrogen phosphate 0.06mol / L;

[0063] (3) adopting a high-speed disperser of 1000 rpm, under the condition that the pH value of the sodium hydroxide control system is 11, the materials of the above-mentioned (1), (2) are fully mixed to form nano-hydroxyapatite sol, Make the molar ratio of calcium and phosphorus in the nano-hydroxyapatite sol system reach 1.65:1, and the content of polyvinylpyrrolidone by mass percentage is 0.5%;

[0064] Step 2: Aging of nano-hydroxyapatite sol

[0065] Move the above-mentioned h...

Embodiment 3

[0074] A method for preparing a nano-hydroxyapatite / chitosan scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0075] Step 1: Preparation of nano-hydroxyapatite sol

[0076] (1) Use polyvinylpyrrolidone (PVP K60) as template agent, dispersant and modifier at the same time;

[0077] (2) use the PVP K60 aqueous solution of 5% by mass percentage to prepare the solution containing calcium nitrate 0.5mol / L, dipotassium hydrogen phosphate 0.3mol / L;

[0078] (3) adopt the high-speed disperser of 2000 revs / min, under the condition that potassium hydroxide control system pH value is 11.5, the material of above-mentioned (1), (2) is fully mixed, forms nano-hydroxyapatite sol, makes The molar ratio of calcium to phosphorus in the nano-hydroxyapatite sol system reaches 1.67:1, and the polyvinylpyrrolidone content is 5% by mass percentage;

[0079] Step 2: Aging of nano-hydroxyapatite sol

[0080] Move the above-mentioned hydroxy...

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Abstract

The invention discloses a preparation method of a nano-hydroxyapatite / chitosan scaffold with oriented arrangement of particles. The method adopts a high-speed disperser to fully mix in the lye control system to form a sol system with a calcium-phosphorus molar ratio of 1.6-1.7:1 and a polyvinylpyrrolidone content of 0.01-50% by mass percentage; after aging and washing, The nano-hydroxyapatite sol: chitosan solute mass ratio is 0.1-9:1, mixed and dispersed at a high speed, poured into a mold and then freeze-dried to obtain a nano-hydroxyapatite / chitosan scaffold. The hydroxyapatite particles in the scaffold are well dispersed in the chitosan matrix and form an effective and stable bond with the chitosan, and present directional arrangements, thereby realizing the structure and function of nano-hydroxyapatite and The effective compounding of chitosan also effectively solves the problems existing in the chitosan material itself. The process of this method is simple, the operation is simple and convenient, and it is easy to popularize and apply.

Description

technical field [0001] The invention relates to the technical field of artificial bone repair materials, in particular to a method for preparing a nano-hydroxyapatite / chitosan scaffold with oriented arrangement of particles. Background technique [0002] Bone defect repair is a research hotspot in the field of clinical medicine and biomaterials at home and abroad. With the development of material science, biomedical technology and the increase of global bone defect cases, bone tissue engineering scaffold materials have become an important branch of bone defect repair materials. [0003] Chitosan (CS for short) is a natural biodegradable polysaccharide, and its degradation product is glucosamine, which is non-toxic and harmless to the human body and tissues, and can promote the adhesion and proliferation of various tissue cells. The drug activity, anti-tumor activity, and anti-inflammatory effect of chitosan can accelerate wound healing, but chitosan lacks bone-bonding biolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/50A61F2/28
Inventor 宁成云尹兆益王迎军郑华德马强任力
Owner SOUTH CHINA UNIV OF TECH