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Biodegradable hydrogel with temperature sensitivity and production method and use thereof

A hydrogel and biological technology, which is applied in the fields of polymer chemistry and biology, can solve the problems of non-degradability, etc., and achieve the effect of simple preparation method and good biocompatibility

Inactive Publication Date: 2009-02-25
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although such hydrogels have good temperature sensitivity, they cannot be completely degraded in vivo

Method used

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  • Biodegradable hydrogel with temperature sensitivity and production method and use thereof
  • Biodegradable hydrogel with temperature sensitivity and production method and use thereof
  • Biodegradable hydrogel with temperature sensitivity and production method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: (1) Preparation of hydroxyethyl methacrylate-polycaprolactone—g-α-(1→6)-D-glucan: a: the hydroxyethyl methacrylate of equimolar ratio (HEMA) and ε-caprolactone (CL) in a nitrogen atmosphere, using stannous octoate as a catalyst, reacted at 110 degrees Celsius for 3 hours, wherein the molar ratio was hydroxyethyl methacrylate: ε-caprolactone: stannous octoate =1:1:0.01. The reacted mixture was dissolved in tetrahydrofuran, and then precipitated in cold water. The precipitate was dissolved in ethyl acetate, dried overnight with magnesium sulfate, and then concentrated under reduced pressure to obtain hydroxyethyl methacrylate-polycaprolactone (PCL-HEMA) . b: Dissolve the obtained product hydroxyethyl methacrylate-polycaprolactone and carbonyldiimidazole (CDI) in tetrahydrofuran at a molar ratio of 1:1, react in a nitrogen atmosphere for 24 hours, and obtain methacrylic acid by rotary evaporation Hydroxyethyl ester - polycaprolactone - imidazole (HEMA-PCL-C...

Embodiment 2

[0022] Embodiment 2: hydrogel controlled release bovine serum albumin (BSA) in vitro

[0023] 20 mg of bovine serum albumin (number average molecular weight: 67,000) was dissolved in 1000 mg of DN2 hydrogel in a phosphate buffer solution, wherein the content of the hydrogel was 8%. Then, the phosphate buffer solution of this hydrogel was fully mixed with bovine serum albumin, placed in a 50 ml centrifuge tube overnight in a water bath at 37° C., so that the hydrogel solution became a viscous hydrogel. 20 ml of phosphate buffer solution warmed to 37° C. was slowly added to the DN2 hydrogel coated with bovine serum albumin. At the set time point, 4 milliliters of phosphate buffer solution was taken out from the 20 milliliter drug delivery system each time, and fresh phosphate buffer solution was added to keep the volume constant. The concentration of BSA therein is detected with an ultraviolet spectrometer, and the ultraviolet absorption wavelength is 280 nanometers, thereby ca...

Embodiment 3

[0024] Example 3: The hydrogel carries bone marrow mesenchymal stem cells and cardiomyocytes and injects them into rats, and compares the differentiation and function of bone marrow mesenchymal stem cells and the differentiation of cardiomyocytes in different environments.

[0025] Seven days after the rats were purchased, they were randomly divided into four groups: 1. myocardial infarction group (8 rats), 2. hydrogel injection group (8 rats), 3. cell injection group (8 rats), 4. injection of Cell and hydrogel groups (11 mice). All injections were made through a 29-gauge needle into the inner portion of the myocardium, and the volume of each injection was 200 microliters. The first group injected 200 microliters of DMEM / F12 medium into the inner layer of the myocardium; the second group injected 200 microliters of hydrogel; the third group injected 200 microliters of suspended 1*10 7 DMEM / F12 medium of bone marrow mesenchymal stem cells; the fourth group was injected with su...

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PUM

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Abstract

The invention relates to a hydrogel which is temperature-sensitive and biodegradable simultaneously, the hydrogel is prepared by the following method: hydroxyethyl methacrylate-polycaprolactone is obtained by the reaction of hydroxyethyl methacrylate and epsilon-caprolactone; the hydroxyethyl methacrylate-polycaprolactone reacts with carbonyldiimidazole, hydroxyethyl methacrylate-polycaprolactone-imidazole is obtained by rotary evaporation; the hydroxyethyl methacrylate-polycaprolactone-imidazole and Alpha-(1->6)-D-glucan are dissolved in dimethyl sulfoxide, p-dimethylaminopyridine is added, mixture which is obtained by the reaction at room temperature is settled in isopropyl alcohol, hydroxyethyl methacrylate-polycaprolactone-g-Alpha-(1->6)-D-glucan is obtained by vacuum drying; N-isopropylacrylamide and the hydroxyethyl methacrylate-polycaprolactone-g-Alpha-(1->6)-D-glucan are dissolved in phosphorous acid buffer solution with pH of 7.4, then ammonium persulfate and N,N,N',N'-tetramethylethylenediamine are added, the hydrogel is obtained by the reaction at room temperature; dialysis is carried out on the obtained hydrogel by phosphorous acid buffer solution, thereby obtaining the temperature-sensitive and biodegradable hydrogel.

Description

technical field [0001] The invention relates to a temperature-sensitive biodegradable hydrogel and its preparation method and application. It belongs to the field of polymer chemistry and also belongs to the field of biology. Background technique [0002] In the 1980s, Tanaka reported that poly N-isopropylacrylamide hydrogel has temperature-sensitive characteristics (J.Chem.Phys.1987, 81:1392), which swells at low temperature and shrinks at high temperature. There is a temperature transition region - the lower critical phase transition temperature (LCST). When the hydrogel is below this temperature, the gel swells, and when the temperature exceeds this temperature, the volume shrinks rapidly. Although such hydrogels have good temperature sensitivity, they cannot be completely degraded in vivo. Contents of the invention [0003] The purpose of the present invention is to provide a hydrogel with both temperature-sensitive characteristics and biodegradable characteristics, ...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14A61K47/30C08F285/00C08G81/00C08G63/08C08G63/91C08B37/02
Inventor 张先正吴德群卓仁禧王涛蒋学俊
Owner WUHAN UNIV
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