Bacterial strain for microbial transformation phytosterin as yield per unit androstane diene diketone

Abstract

The invention discloses phytosterol of microbial transformation, which is a strain which only produces androstene dione, pertaining to the fermentation technology field in biological engineering. The screened strains are classified and named as starch degrading bacillus (Bacillus a myloiquefaciens.) ST 06-95 and preserved in the China Center for Type Culture Collection with a preservation number of CCTCC No. M208135. Samples are collected from the nature separated for primary screening and put in slant preservation; wild strains ST 06 are taken as starting strains and screened by ultraviolet mutagenesis to obtain strains ST06-95 which mainly produce androstenone; and the growth of strains enters a logarithmic growth phase at the temperature of 30 DEG C, the pH value of 7.0 and a rotation speed of 220r / min and after culture lasting for 60h. Thallus in the growth phase are inoculated into a fermentation culture medium according to inoculation amount of 12 percent (V / V) and fermented for 6 days to 7 days at the temperature of 30 DEG C, the pH value of 7.0 and a rotation speed of 220r / min. When quantitative detection is carried out on the fermented products, the product content of ADD exceeds 1500mg / l, and ADD accounts for 98 percent of the total amount of AD and ADD. The microbial transformation provides a basis for the industrialization of the microbial transformation of steroidal medicaments.

Description

technical field

[0001] The invention relates to the production of androstadienedione, a key intermediate of steroid medicine, by transforming phytosterols with microorganisms, and belongs to the technical field of fermentation in bioengineering. Background technique

[0002] In 1949, Hench et al. found that cortisone had outstanding curative effect on rheumatoid arthritis, and confirmed that both cortisone and hydrocortisone belonged to adrenal cortex hormones, making steroid corticosteroids a drug with high curative effect and high economic value. Because the content of hormones in animals is extremely low, it cannot be prepared in large quantities by directly extracting from animal raw materials. At present, semi-synthetic methods are mostly used, that is, raw materials with steroidal nucleus cyclopentane polyhydrophenanthrene are obtained from animals and plants, and then prepared by enzymatic transformation and chemical transformation. Sterols, the starting raw material...

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