Enzyme immunosensor for detecting Shigella species and its preparation method and application

A Shigella and enzyme immunization technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of complex detection process, long detection time, human and environmental hazards, etc., and achieve simplified preparation steps, short response time, highly specific effect
CN101532980AInactive Publication Date: 2009-09-16ZHEJIANG GONGSHANG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG GONGSHANG UNIVERSITY
Publication Date
2009-09-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a sensitive, rapid, accurate, high-specificity and economical enzyme immunosensor for detecting Shigella species, comprising screen printing electrodes, a sensitive membrane containing enzyme marked Shigella species antibody- carbon nano-tube- shitosan mixture is coated on the working electrode of the screen printing electrodes. In the invention, the chemical amplification function of the multi-wall carbon nano-tube and enzyme is combined with the specificity of the immunosensor, theenzyme immunosensor has the specificity of immunoreactivity and the sensitivity of the electrochemistry analysis and can accurately detect the lower-content substance. The invention also discloses a preparation method and application of the enzyme immunosensor, the Shigella species can be directly detected, with the sensitive, rapid, high-specificity advantages, enzyme immunosensor is cheap and suitable for detecting the Shigella species in the foundation and in situ.
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Description

technical field

[0001] The invention relates to the technical field of rapid detection of food-borne pathogenic bacteria, in particular to an enzyme immunosensor for detecting Shigella based on an antigen-antibody immune reaction and a preparation method and application thereof. Background technique

[0002] Among the hundreds of millions of food-borne disease patients in the world every year, 70% are caused by eating food and drinking water contaminated by various pathogenic microorganisms. Shigella (Shigella) is one of the main pathogens that cause acute infectious diarrhea. As few as 10 bacteria can cause infection, and the infected human body will have symptoms such as nausea, vomiting, abdominal pain, diarrhea (severe pus and blood in the stool), and even endanger the human body. life. Shigella infection kills more than one million people every year, mostly children in developing countries.

[0003] The rapid detection of Shigella is one of the most critical measures ...

Claims

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