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Method for preparing 2, 3-butanediol using xylose mother liquor

A technology of xylose mother liquor and butanediol, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as no reports, and achieve the effects of rapid regeneration cycle, cost reduction, and low cost.

Inactive Publication Date: 2009-10-07
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fermentative production of 2,3-butanediol mainly focuses on utilizing glucose as a substrate (SyuMJ. Biological production of 2, 3-butanediol. Appl Microbiol Biotechnol, 2001, 55: 10-18; 3-butanediol by Klebsiella pneumoniae using glucose and ammoniumphosphate. Chin J Chem Eng, 2006, 14: 132-136), a patent for fermentative production of 2,3-butanediol using industrial by-product raw materials containing pentose and hexose sugars as substrates not yet reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of seed solution of Klebsiella pneumoniae (Klebsiella pneumoniae) CICC 10011 strain

[0036] Klebsiella pneumoniae (Klebsiella pneumoniae) CICC 10011 (purchased from the China Industrial Microbiology Culture Collection Center) was inoculated on the slant medium, and cultured at 35°C for 14 hours. Pick 2 loops from the inoculation loop and place them in a 500mL Erlenmeyer flask containing 100mL of liquid seed medium, place them on a shaker with a rotation speed of 150rpm, and incubate at 37°C for 10 hours to obtain Klebsiella pneumoniae (Klebsiella pneumoniae) CICC 10011 The seed liquid of the strain.

[0037] The above slant medium is: glucose 20g / L, peptone 3g / L, yeast extract 4g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, sodium chloride 3g / L, magnesium sulfate 0.1g / L L, zinc sulfate 0.1g / L, agar powder 22g / L, pH adjusted to 7.0, prepared with distilled water, sterilized at 115°C for 20 minutes.

[0038] The above seed me...

Embodiment 2

[0040] Preparation of seed solution of Klebsiella pneumoniae (Klebsiella pneumoniae) ATCC 8724 strain

[0041] Klebsiella pneumoniae (Klebsiella pneumoniae) ATCC 8724 (purchased from the American Standard Biological Collection) was inoculated on the slant medium, and cultured statically for 12 hours at 37°C. Pick 1 ring and put it in a 500mL Erlenmeyer flask equipped with 80mL liquid seed medium, place it on a shaker with a rotation speed of 100rpm, and cultivate it at 35°C for 14 hours to obtain Klebsiella pneumoniae (Klebsiella pneumoniae) ATCC 8724 bacterial strain seed liquid.

[0042] The above slant medium is: glucose 10g / L, peptone 5g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 3g / L, potassium dihydrogen phosphate 3g / L, sodium chloride 1g / L, magnesium sulfate 0.3g / L L, zinc sulfate 0.3g / L, agar powder 20g / L, pH adjusted to 6.0, prepared with distilled water, sterilized at 115°C for 20 minutes.

[0043] The above seed medium is: glucose 40g / L, dipotassium hyd...

Embodiment 3

[0045] Production of 2,3-Butanediol by Fermentation of Klebsiella pneumoniae CICC 10011 in Erlenmeyer Flask

[0046] With the seed solution prepared in Example 1, under aseptic conditions, an inoculum size of 5% by volume is inserted into a 500mL Erlenmeyer flask with a liquid content of 100mL of liquid fermentation medium, placed on a shaking table with a rotation speed of 120rpm, at 30°C Cultivate for 30 hours, wherein the fermented liquid is taken every 3 hours to measure the residual sugar and the concentration of 2,3-butanediol in the fermented liquid. After the fermentation, the concentration of 2,3-butanediol is 43.7g / l, the conversion rate is 87.4% of the theoretical conversion rate.

[0047] The above fermentation medium is: the initial total sugar concentration is 100g / L, triammonium citrate 3g / L, diammonium hydrogen phosphate 15g / L, sodium acetate 2g / L, potassium chloride 1g / L, magnesium sulfate 0.05g / L , zinc sulfate 0.10g / L, manganese sulfate 0.05g / L, ferrous su...

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Abstract

The invention discloses a method for preparing 2,3-butanediol using xylose mother liquor, which utilizes the xylose mother liquor as a fermentation substrate, uses klebsiella pneumoniae as a fermentation strain and produces and prepares the 2,3-butanediol by batch fermentation and feed batch fermentation. By the method, the final concentration of the 2,3-butanediol reaches 78.9g / L, total sugar conversion rate reaches more than 85 percent, and the maximum productivity of the 2,3-butanediol is 1.3g / (L / h). In the light of the day-by-day exhaustion of petroleum resources and the rise of the price of petrochemical products, the invention utilizes renewable agricultural byproduct corncob for deep processing to produce the xylose mother liquor as the byproduct of xylitol and produces high-value platform compound 2, 3-butanediol by microbial fermentation, thus realizing the post-development and high-value utilization of the xylose mother liquor as the industrial byproduct of the xylitol.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for preparing 2,3-butanediol by using industrial by-product xylose mother liquor. Background technique [0002] 2,3-butanediol (2,3-butanediol) is a potential high value-added platform compound. In recent years, due to the instability of oil prices and the rising prices of petrochemical products, the price and production of 2,3-butanediol have attracted more and more attention internationally. 2,3-butanediol is a colorless and odorless chiral compound with a molecular weight of 90.12KDa, a boiling point of 180-184°C and a low freezing point. D-2,3-butanediol (-60°C) can be used as an anti refrigerant. 2,3-Butanediol has a high octane number and can be used to produce high-grade aviation kerosene. Dehydration can be converted into industrial solvent methyl ethyl ketone, methyl ethyl ketone can be used as a fuel additive, and can be used as a so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 马翠卿王爱龙王钰李理想姜天翼唐鸿志许平
Owner SHANDONG UNIV
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